osteoarthritic cartilage
Repression of anti proliferative factor Tob1in osteoarthritic cartilage
... from osteoarthritic cartilage (10 sam- ples) and pooled normal cartilage was amplified and labeled with Cy3-UTP and Cy5-UTP respectively (Amer- sham Pharmacia) using the MessageAmp aRNA kit ...from ...
11
Mathematical modelling of cytokines, MMPs and fibronectin fragments in osteoarthritic cartilage
... human cartilage, and could be extended to investigate whether this changes in osteoarthritic ...increased cartilage degradation have been reported (Manicourt et ...
40
The role of aggrecan in normal and osteoarthritic cartilage
... articular cartilage with its ability to withstand compressive ...to cartilage function and are enhanced in osteoarthritic cartilage, resulting in aggrecan depletion and predisposing to ...
11
Extrusion of pyrophosphate into extracellular media by osteoarthritic cartilage incubates
... articular cartilage in chondrocalcinosis, and the high level of pyrophosphate (PPi) ion relative to serum in synovial fluid in patients with either chondrocalcinosis or advanced osteoarthritis led to an ...
9
Expression of 92 kD type IV collagenase/gelatinase (gelatinase B) in osteoarthritic cartilage and its induction in normal human articular cartilage by interleukin 1
... human osteoarthritic cartilage, but not in normal adult articular ...normal cartilage also exhibited mRNA for 72-kD type IV collagenase/gelatinase (gelatinase A), tissue collagenase, and ...
8
Increased expression of lipocalin type prostaglandin D2synthase in osteoarthritic cartilage
... Chondrocytes were released from cartilage by sequential enzymatic digestion as previously described [29]. Briefly, this consisted of 2 mg/mL pronase for 1 hour followed by 1 mg/mL collagenase for 6 hours (type IV; ...
12
Treatment of posttraumatic and focal osteoarthritic cartilage defects of the knee with autologous polymer based three dimensional chondrocyte grafts: 2 year clinical results
... In this case series we demonstrated the benefit and reliability of the gel-polymer-based chondrocyte graft BioSeed-C for the treatment of posttraumatic and degenerative large full-thick- ness cartilage lesions of ...
11
Expression of Cannabinoid Receptors in Human Osteoarthritic Cartilage: Implications for Future Therapies
... of cartilage, although this failed to reach significance and immunopositivity was pre- dominantly ...OA cartilage and PPARc expression has been shown to be nuclear in rat ...of cartilage lesions 60,61 ...
13
Evidence for metalloproteinase and metalloproteinase inhibitor imbalance in human osteoarthritic cartilage
... approximately 150% in OA; TIMP was elevated approximately 50%. A positive correlation (r = 0.50) was found between acid and neutral proteinase activities in OA, but not in controls. Both proteinases were elevated two-to ...
9
Peroxisome proliferator activated receptor γ1 expression is diminished in human osteoarthritic cartilage and is downregulated by interleukin 1β in articular chondrocytes
... OA cartilage is of considerable interest, given the protective functions of PPAR γ in ...as cartilage degradation ...OA cartilage may, at least in part, be involved in increased expression of ...
11
High resolution optical coherence tomographic imaging of osteoarthritic cartilage during open knee surgery
... A representative OCT image and the corresponding histol- ogy of normal knee articular cartilage are shown in Fig. 2. The OCT image (Fig. 2a) reveals that the cartilage was thick and uniform with a rather ...
6
Expression of cannabinoid receptors in human osteoarthritic cartilage: implications for future therapies
... Other workers have demonstrated expression of CB1 and CB2 by bovine articular chondrocytes, 20 synovia of patients with OA and RA, 30 and human chondrocytes and fibroblast-like synoviocytes. 19,39 Here, we demon- strate ...
14
Expression of minor cartilage collagens and small leucine rich proteoglycans may be relatively reduced in osteoarthritic cartilage
... OA cartilage, we conducted ultrastructural observation of collagen fibrils in OA and control cartilage by ...OA cartilage, collagen fibrils were placed randomly in direc- tions around chondrocytes in ...
10
Hyaluronic Acid-Based Optical Probe for the Diagnosis of Human Osteoarthritic Cartilage
... human osteoarthritic tissue before OCT ...human osteoarthritic tissue taken after being incubated with FA-HA probes for 30 ...human osteoarthritic tissue were determined statistically with a Pearson ...
13
GREM1, FRZB and DKK1 mRNA levels correlate with osteoarthritis and are regulated by osteoarthritis associated factors
... articular cartilage ’ s natural brakes of hypertrophic ...articular cartilage from healthy preadolescents and healthy adults as well as in preserved and degrading osteoarthritic cartilage from ...
11
Collagenase and collagenase inhibitors in osteoarthritic and normal cartilage
... demonstrate the presence of the enzyme; but the same culture fluid, incubated with trypsin, showed significant degradation of collagen, suggesting that trypsin destroyed the inhibitor. 7-Day culture fluids were then ...
9
Articular cartilage and changes in Arthritis: Cell biology of osteoarthritis
... and osteoarthritic cartilage specimens, using the markers for chondrocyte differentiation, colla- gen type II and aggrecan (activated functional chondro- cytes), collagen types I and III (dedifferentiated ...
7
The oncofetal gene survivin is re expressed in osteoarthritis and is required for chondrocyte proliferation in vitro
... arthritic cartilage (A, C, E) and adult non/moderate arthritic cartilage (paraffin-embedded tissue sections) (B, D, ...(G) Cartilage from 10 patients was analyzed for the mRNA expression of survivin ...
8
Collagen synthesis in normal and osteoarthritic human cartilage
... into osteoarthritic cartilage was increased fourfold as compared to normal cartilage and varied with advancing histological-histochemical ...the cartilage matrix, as an index of the turnover ...
9
Cartilage matrix glycoprotein is present in serum in experimental canine osteoarthritis
... 550,000-D cartilage matrix glycoprotein (CMGP), which is found in normal hyaline cartilage, fibrocartilage, and the vitreous of the eye, and consists of subunits with apparent molecular weights of 130,000 ...
9