p21‑activated kinase 4
Original Article p21-activated kinase 4 promotes proliferation, migration, and invasion of human gastric cancer cells
... Background/Aim: p21-activated kinase 4 (PAK4) overexpression has been observed in many cancers, including metastatic gastric cancer ...
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Overexpression of P21-activated kinase 4 is associated with poor prognosis in non-small cell lung cancer and promotes migration and invasion
... c-Src/mitogen- activated protein kinase kinase 1 (MEK1)/extracellular signal-regulated kinase (ERK)1/2 and MMP-2 in ovar- ian cancer [16], LIMK1/cofilin in prostate cancer [17], MMP-2 in ...
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Activation of p21-Activated Kinase 2 by Human Immunodeficiency Virus Type 1 Nef Induces Merlin Phosphorylation
... PAK2 kinase activity and trans-phos- phorylation of GST-tagged ...PAK2 activated by SF2 Nef outside the context of the cellular milieu, we also used a re- cently described cell-free system to translate Nef ...
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A Hydrophobic Binding Surface on the Human Immunodeficiency Virus Type 1 Nef Core Is Critical for Association with p21-Activated Kinase 2
... vates Pak2 through a multiprotein complex (4, 26, 33, 47). Pulkkinen et al. demonstrated that Pak2 activation occurs in two mechanistically distinct steps, association and activation, that are nearly concurrent ...
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p21-activated kinase 1 (PAK1) expression correlates with prognosis in solid tumors: A systematic review and meta- analysis
... p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1) is a member of the PAK family of proteins, which are effectors of small Rho GTPases (Cdc42 and Rac1) [1, 2]. PAK1 is involved in a variety of ...
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Reconstitution and Molecular Analysis of an Active Human Immunodeficiency Virus Type 1 Nef/p21-Activated Kinase 2 Complex
... histone 4 (H4) (calf thymus; Roche Diagnos- tics, Mannheim, Germany) was assayed by the addition of substrate (10 g) to anti-Nef immunoprecipitates prior to the in vitro kinase assay ...at 4°C; ...
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Human immunodeficiency virus type 1 Nef associates with a member of the p21-activated kinase family.
... Nef-associated kinase identify it as a member of the PAK ...S6/H4 kinase identify it as belonging to the second PAK (PAK65/PAK2/ g PAK) subfamily ...Nef-associated kinase comi- grates with the 65-kDa ...
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Regulation of Phosphoinositide Levels by the Phospholipid Transfer Protein Sec14p Controls Cdc42p/p21-Activated Kinase-Mediated Cell Cycle Progression at Cytokinesis
... The role of the Saccharomyces cerevisiae phosphatidylcho- line (PC)/phosphatidylinositol (PI) transfer protein Sec14p as an essential regulator of Golgi apparatus-derived vesicular transport is well established (3–5, 10, ...
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Association of Nef with p21-Activated Kinase 2 Is Dispensable for Efficient Human Immunodeficiency Virus Type 1 Replication and Cytopathicity in Ex Vivo-Infected Human Lymphoid Tissue
... The accessory Nef protein of human and simian immuno- deficiency viruses (HIV and SIV, respectively) is expressed at high levels early during the viral life cycle and is critical for efficient viral replication and ...
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Gringel, Alexandra (2009): Regulation podosomaler Adhäsionen in primären humanen Makrophagen durch p21-activated kinase (PAK)-abhängige Signalwege. Dissertation, LMU München: Medizinische Fakultät
... Bezüglich des strukturellen Aufbaus gibt es zwischen PAK1 und PAK4 neben einigen Gemeinsamkeiten auch deutliche Unterschiede (s. auch 1.4.2). Zu nennen sind hier die Abwesenheit der AID und der Acidic Region im PAK4 ...
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Activation of P21-activated protein kinase 2 is an independent prognostic predictor for patients with gastric cancer
... The p21-activated kinases (PAKs) are a family of serine/ threonine protein kinases, which were initially identified as binding partners of the Rho GTPases Cdc42 and Rac1 ...(PAKs 4–6) based on ...
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Group A p21-activated kinases in radial neuronal migration
... 2- 4 days, and the radial distribution of cells expressing the electroporated constructs was ...including kinase dependent and independent effects, on cell morphology and ...motility. Kinase ...
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The p21 activated kinase Mbt is a component of the apical protein complex in central brain neuroblasts and controls cell proliferation
... Fig. 4. Loss of Mbt does not disturb the cytoskeleton. Projections of confocal sections of third instar brains stained for either Actin/Mir/PH3, α- Tubulin (α-Tub)/aPKC/PH3 or Armadillo (Arm)/aPKC/PH3. Neuroblasts ...
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Down-regulation of p21-activated serine/threonine kinase 1 is involved in loss of mesencephalic dopamine neurons
... perfusion with 4 % paraformaldehyde solution through the heart. Total brain was isolated and additionally incu- bated with 4 % paraformaldehyde solution for 24 h. Then brain tissues were incubated with 20 % ...
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Essential oil of Pinus koraiensis inhibits cell proliferation and migration via inhibition of p21 activated kinase 1 pathway in HCT116 colorectal cancer cells
... Pinus koraiensis, generally called the Korean nut pine, is an evergreen tree found in Korea, China, Japan, and eastern Russia. Unlike the common Pinaceae plants, P. koraiensis shows leaves (needles) in fascicles ...
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Nef Associates with p21-Activated Kinase 2 in a p21-GTPase-Dependent Dynamic Activation Complex within Lipid Rafts
... Raft fractionation by membrane flotation. Cells were washed once with PBS and lysed with ice-cold isolation buffer (150 mM NaCl, 5 mM EDTA, 25 mM Tris-HCl [pH 7.4], 1% Triton X-100, 1 mM sodium orthovanadate, 1 mM ...
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PakD, a Putative p21-Activated Protein Kinase in Dictyostelium discoideum, Regulates Actin
... pakD ⴚ cells express the developmentally regulated gene carA, encoding cAR1. The developmental program of D. discoi- deum requires the expression of developmental genes such as carA, which encodes the cell surface ...
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p21-activated kinase 1: PAK’ed with potential
... a p21-binding domain (PBD), a serine/threonine kinase domain, an acidic region and multiple proline-rich regions that serve as binding sites for SH3 domain-containing ...
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p21 activated kinase signaling in breast cancer
... ER activation by Pak1 also induces upregulation of cyclin D 1 in breast cancer cells, as well as in the mammary epithelium [57]. In addition, Pak1 could also upregulate cyclin D 1 expression in an ER-independent, but NF- ...
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Nef mediated enhancement of cellular activation and human immunodeficiency virus type 1 replication in primary T cells is dependent on association with p21 activated kinase 2
... again at 1000 × g for 10 min. The cell pellet was resus- pended in 10 mls of RPMI-1640 (Invitrogen) and pel- leted at 1000 × g for 10 min. Cells were counted and resuspended at 2 × 10 6 /ml in RPMI-1640 with 10% pooled ...
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