Panc1 cells
Cytoplasmic p21 induced by p65 prevents doxorubicin-induced cell death in pancreatic carcinoma cell line
... on PANC1 cells, correlating with increased induction of cytoplasmic ...the PANC1 cells were more sensitive to DOX treatment, correlated with activation of caspase-3 and clear elevation of ...
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Transcriptional repression of SOCS3 mediated by IL-6/STAT3 signaling via DNMT1 promotes pancreatic cancer growth and metastasis
... cancer cells, we used wound healing and transwell invasion ...h, Panc1 cells transfected with siRNA against SOCS3 occupied approximately 77 % of the wound, while Panc1 cells with ...
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Apoptosis signal-regulating kinase 1 exhibits oncogenic activity in pancreatic cancer
... from PANC1 cells treated with control or ASK1-targeted siRNAs (Figure ...from PANC1 cells treated with ASK1-targeted siRNAs grew more slowly than those from cells treated with the ...
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GSK3β mediates pancreatic cancer cell invasion in vitro via the CXCR4/MMP-2 Pathway
... PANC1 cells were lysed using RIPA buffer. Equal amounts of protein (60 μg) were separated on 10 % SDS-PAGE gels and transferred to Immobilon-P membranes (Millipore, Bedford, MA, USA). The membranes were ...
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<p>TMEFF2 inhibits pancreatic cancer cells proliferation, migration, and invasion by suppressing phosphorylation of the MAPK signaling pathway</p>
... and Panc1 cells were ...and Panc1 cells of the TMEFF2 group exhibited much lower OD450 values, colony number, tumor volume and weight, migration and invasion cell numbers, obviously higher ...
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Fendiline inhibits proliferation and invasion of pancreatic cancer cells by interfering with ADAM10 activation and β-catenin signaling
... cancer cells are known to undergo EMT (Epithelial to mesenchymal transition), which promotes their invasive and metastatic potential [48, ...that Panc1 cells express N-Cadherin [19]; fendiline ...
18
Complementary Induction of Immunogenic Cell Death by Oncolytic Parvovirus H-1PV and Gemcitabine in Pancreatic Cancer
... PDAC cells as determined by immunofluorescence using antibodies targeting the viral nonstructural protein NS1 upon exposure of PDAC cells to H-1PV at an MOI of 10 PFU/cell for 48 h (magnification, ⫻ ...
14
FOXO1-regulated lncRNA LINC01197 inhibits pancreatic adenocarcinoma cell proliferation by restraining Wnt/β-catenin signaling
... FOXO1-negative cells carry cancer stem-like characteristics in PDAC [10] and affect tumor progression, suggesting that FOXO1 func- tions as a tumor suppressor in PDAC; however, the underlying mechanism remains ...
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The long non-coding RNA HOTTIP enhances pancreatic cancer cell proliferation, survival and migration
... in Panc1 pancreatic cancer cells decreased proliferation, induced apoptosis and decreased ...In Panc1 cells transfected with siHOTTIP, there was a decrease in expression of 757 genes and ...
13
Sialylation transmogrifies human breast and pancreatic cancer cells into 3D multicellular tumor spheroids using cyclic RGD- peptide induced self-assembly
... cancer cells and their chemoresistant variants to form spheroids was studied using the RGD- peptide-based platform which causes specific biochemical alterations of cell surface ...MCF-7 cells formed tight ...
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lncRNA differentiation antagonizing nonprotein coding RNA overexpression accelerates progression and indicates poor prognosis in pancreatic ductal adenocarcinoma
... of Panc1 cells with DANCR deficiency were significantly suppressed in the Transwell assay and the Matrigel ...BxPC3 cells with DANCR overexpression was obviously ...of Panc1 cells in ...
11
Metabolic regulation of calcium pumps in pancreatic cancer: role of phosphofructokinase-fructose-bisphosphatase-3 (PFKFB3)
... and PANC1 cells were obtained from the ATCC (Manassas, Virginia, USA), whereas human pan- creatic stellate cells, isolated from resected tumour tissue (HPSCs) were a kind gift from Professor David ...
11
Selective impact of CDK4/6 suppression on patient-derived models of pancreatic cancer
... epithelial cells. The mutual interaction between tumor cells and surrounding environment appears particularly significant in PDA, where stromal cell promote tumor development and invasiveness but, in some ...
14
Sialylation facilitates self-assembly of 3D multicellular prostaspheres by using cyclo-RGDfK(TPP) peptide
... DU145GemR cells formed small MCTS. In contrast, PC3 and PC3GemR cells formed irregular multicellular aggregates at all concentrations of cyclo- RGDfK(TPP) peptide, even after 6 days of ...DU145 cells ...
21
Micro-RNA in pancreatic adenocarcinoma: predictive/prognostic biomarkers or therapeutic targets?
... There are many other miRNA which have been studied in preclinical studies. MiRNA-204 is a putative regulator of myeloid cell leukemia-1 (Mcl-1), and in PDAC there are low levels of this miRNA and an overexpression of ...
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Gemcitabine and Doxorubicin Combination Enhance the Cytotoxic Effect to Pancreatic Cancer Cells BxPC3 and PANC1 through UMP/CMP Kinase 1
... Pancreatic cancer is fatal for patients with a median survival of less than 6 months [1], and has become one of the most devastating and deadly forms of cancers, and has an aggressive course predominantly seen in men at ...
11
<p>Knockdown of HMGB1 Suppresses Hypoxia-Induced Mitochondrial Biogenesis in Pancreatic Cancer Cells</p>
... lines PANC1, CFPAC1, BXPC-3 and AsPC-1 American Type Culture Collection (Manassas, VA, USA); human bron- chial epithelial cell HBE, acute myeloid leukemic cell HL-60 and chronic myelogenous leukemic cell K562 ...
12
Dendritic cells fused with different pancreatic carcinoma cells induce different T-cell responses
... with fused DCs or DCs alone for 14 days were washed, and cytospins from PBMCs were air-dried and fixed for 10 minutes in acetone. Endogenous peroxidase activity was blocked with methanol containing 0.3% hydrogen ...
12
FOLFOX regimen plus dendritic cells–cytokine-induced killer cells immunotherapy for the treatment of colorectal cancer: a meta-analysis
... Data were extracted by two researchers independently (Yan Liu and Zhong Zheng), and discrepancy was resolved upon discussing with a third researcher (Qixin Zhang). Extracted information included first author’s names, ...
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Mast cells positive to tryptase, endothelial cells positive to protease-activated receptor-2, and microvascular density correlate among themselves in hepatocellular carcinoma patients who have undergone surgery
... mast cells positive to the antitryptase ...mast cells. Big arrows indicate single mast cells adjacent to a microvessel and double small arrows indicate red immunostained tryptase forming a ...
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