Papain-like protease
Structural and functional characterization of MERS coronavirus papain-like protease
... Backgrounds: A new highly pathogenic human coronavirus (CoV), Middle East respiratory syndrome coronavirus (MERS-CoV), has emerged in Jeddah and Saudi Arabia and quickly spread to some European countries since September ...
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Hypovirus Papain-Like Protease p29 Suppresses RNA Silencing in the Natural Fungal Host and in a Heterologous Plant System
... exhibit papain-like protease activity and share striking similarities in the sequences surrounding the catalytic cysteine and histidine residues and the spacing of these essential residues relative ...
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Structurally Guided Removal of DeISGylase Biochemical Activity from Papain-Like Protease Originating from Middle East Respiratory Syndrome Coronavirus
... a papain-like protease (PLpro) that possesses the ability to antago- nize interferon immune pathways through the removal of ubiquitin and interferon- stimulated gene product 15 (ISG15) from target ...
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The Papain-Like Protease of Severe Acute Respiratory Syndrome Coronavirus Has Deubiquitinating Activity
... proteases, papain-like protease (PLpro) and 3C-like protease ...transfer-based protease assays. We performed bioinformatics analysis on 16 papain-like ...
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Murine Coronavirus Ubiquitin-Like Domain Is Important for Papain-Like Protease Stability and Viral Pathogenesis
... the papain-like protease (PLP) domains of coronaviruses (CoVs) revealed an adjacent Ubl domain in severe acute respiratory syndrome CoV, Middle East respiratory syndrome CoV, and the murine CoV, ...
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Identification of Severe Acute Respiratory Syndrome Coronavirus Replicase Products and Characterization of Papain-Like Protease Activity
... Gene 1 of the coronavirus associated with severe acute respiratory syndrome (SARS) encodes replicase poly- proteins that are predicted to be processed into 16 nonstructural proteins (nsps 1 to 16) by two viral proteases, ...
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The Papain-Like Protease from the Severe Acute Respiratory Syndrome Coronavirus Is a Deubiquitinating Enzyme
... The identification of a coronavirus as the infectious agent of severe acute respiratory syndrome (SARS) and the rapid se- quencing of its genome have led to a renewed interest in coronaviruses (34). SARS, which emerged ...
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Hypovirus Papain-Like Protease p48 Is Required for Initiation but Not for Maintenance of Virus RNA Propagation in the Chestnut Blight Fungus Cryphonectria parasitica
... a papain-like cysteine protease domain located within the p29 coding ...N-terminal papain-like protease, p48, and conserved poly- merase and helicase domains (modified from ...
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Catalytic Function and Substrate Specificity of the Papain-Like Protease Domain of nsp3 from the Middle East Respiratory Syndrome Coronavirus
... antagonist papain-like protease (PLpro) ...cysteine protease that hydrolyzes peptide and isopeptide bonds in viral and cellular sub- strates, an essential function for coronavirus ...
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Papain-Like Protease 1 from Transmissible Gastroenteritis Virus: Crystal Structure and Enzymatic Activity toward Viral and Cellular Substrates
... or papain-like ...The papain-like protease 1 (PL1 pro ) domain is present in nonstructural protein 3 (nsp3) of alphacoronaviruses and subgroup 2a ...site. Like its PL2 pro ...
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The SARS coronavirus papain like protease can inhibit IRF3 at a post activation step that requires deubiquitination activity
... PLpro is one of the two virally encoded proteases that are necessary for SARS-CoV to cleave the coronavirus ORF1A polyprotein into separate peptides that function in replication of SARS-CoV genomic RNA [26]. PLpro has ...
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Severe Acute Respiratory Syndrome Coronavirus Papain-Like Protease Ubiquitin-Like Domain and Catalytic Domain Regulate Antagonism of IRF3 and NF-κB Signaling
... Role of the catalytic domain in IFN antagonism. The cata- lytic region of PLP plays a role in IFN antagonism as well. Mutagenesis of the catalytic triad in the active site differentially affected IFN antagonism (Fig. 9). ...
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Papain-like protease p29 as a symptom determinant encoded by a hypovirulence-associated virus of the chestnut blight fungus.
... EP155 spheroplasts were transformed with pCPXHYI derivatives containing either the fulllength L-dsRNA cDNA clone plasmid pXH9, yielding the transformant designated HAV or the L-dsRNA cDN[r] ...
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Deubiquitinating and Interferon Antagonism Activities of Coronavirus Papain-Like Proteases
... proteases, papain-like (PLPs/PLpro) and picornavirus 3C- like (3CLpro), to generate mature nonstructural proteins (nsp’s) that assemble with host cell membranes to form double membrane vesicles ...
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A Chimeric Virus-Mouse Model System for Evaluating the Function and Inhibition of Papain-Like Proteases of Emerging Coronaviruses
... viral protease activities and the efficacy of protease inhibitors is a high ...the papain-like protease (PLpro) of severe acute respiratory syn- drome coronavirus (SARS-CoV), a ...
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zjv11825.pdf
... viral papain-like protease domain, and synthetic medicinal chemistry and structure-activity relation- ship studies have produced compounds that inhibit replication of SARS-CoV in cell culture (13, ...
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In silico Repositioning for Dual Inhibitor Discovery of SARS-CoV-2 (COVID- 19) 3C-like Protease and Papain-like Peptidase
... Coronavirus virus is an enveloped positive-sense single-stranded RNA that eventually cause systemic changes and damage in many vital organs (8). Similar to all viruses, two-thirds of the viral RNA is translated into two ...
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Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 18 May 2020 doi:10.20944/preprints202005.0295.v1
... tract) was better than the temperature of respiratory tract. These results displayed that EGCg has very good anti-BCV activity, it interacts with BCV particles and interferes with the adsorption of BCV to MDBK cells, ...
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Virtual screening and molecular docking of Anti-Antileishmanial for selected pharmacophore for visceral Leishmaniasis
... Cysteine protease DNA of Leishmania donovani was successfully amplified by ...cysteine protease has been done and docked with suitable inhibitors by using various servers and computational ...
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Chemical Analysis of Carica papaya L Crude Latex
... which papain was most active ...of papain are found to be pH = 5 ...and papain from papaya, have proteolytic activity [42]. Papain as a cysteine hydrolase is stable and active under a wide ...
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