Papillomavirus E2 protein
Interaction of Bovine Papillomavirus E2 Protein with Brd4 Stabilizes Its Association with Chromatin
... bovine papillomavirus E2 protein maintains and segregates the viral extrachromosomal genomes by tethering them to cellular mitotic ...chromosomes. E2 interacts with a cellular bromodomain ...
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Evolutionary variation of papillomavirus E2 protein and E2 binding sites
... PV E2 proteins have evolved different affinities and different preferences for E2BSs, including spacer nucleotides, which control E2BS ...the E2 protein function has been performed on domains from a ...
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Phosphorylation of the Bovine Papillomavirus E2 Protein on Tyrosine Regulates Its Transcription and Replication Functions
... equal protein levels were measured, as determined by a bicinchoninic acid (BCA) assay (Pierce Thermo ...anti-BPV-1 E2), M2 (mouse anti-FLAG; Sigma), 502-2 (rabbit anti-BPV-1 E1), HA-7 (mouse anti-HA; ...
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Human Papillomavirus E2 Protein: Linking Replication, Transcription, and RNA Processing
... that E2, in addition to having DNA-related roles, can bind RNA and has properties and functions similar to those of SRSF ...-type E2 proteins is rich in serine and arginine residues and can be ...
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The Mitotic Chromosome Binding Activity of the Papillomavirus E2 Protein Correlates with Interaction with the Cellular Chromosomal Protein, Brd4
... BPV1 E2 transactivation domain and identifi- cation of targets for ...BPV1 E2 protein has 34% identity with that of the HPV16 E2 ...BPV E2 model was scanned for patches of surface ...
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Identification and Analysis of Papillomavirus E2 Protein Binding Sites in the Human Genome
... N294A E2 proteins and high-risk HPV18 E2 ...HPV18 E2 proteins but not by HPV11 N294A E2, which is defec- tive in sequence-specific DNA ...of E2 did not alter any cellular transcript ...
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Proteasomal Degradation of the Papillomavirus E2 Protein Is Inhibited by Overexpression of Bromodomain-Containing Protein 4
... of E2 using a dominant-negative Brd4 construct (Brd4-N) and, in separate experiments, by downregulation of Brd4 with a ...inhibits E2 trans- activation despite the fact that it lacks the E2-binding ...
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Phosphorylation of a Conserved Tyrosine in the Papillomavirus E2 Protein Regulates Brd4 Binding and Viral Replication
... pSG5-HA-HPV-31 E2 (58), codon-optimized pCDNA3-FLAG-HPV-31 E2, pCDNA-FGFR3 K650E ...pSG5-HA-HPV-31 E2 and the pDCDNA3-FLAG-HPV-31 E2 constructs using the Quick-Change XL Lightning kit (Agilent ...
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NRIP, a Novel Calmodulin Binding Protein, Activates Calcineurin To Dephosphorylate Human Papillomavirus E2 Protein
... increased E2 protein levels in a dose-dependent manner but did not alter the expression level of E2 transcripts regardless of NRIP plasmid transfection dos- age ...of E2 proteins caused by ...
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A Novel Silencer Element in the Bovine Papillomavirus Type 4 Promoter Represses the Transcriptional Response to Papillomavirus E2 Protein
... Plasmid constructs and expression vectors. Construction of the PV2 6E2 and tk 6E2 reporter constructs has been described previously (39). The lcr/tk hybrid promoter was generated by splicing-by-overlap-extension PCR. The ...
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The SMC5/6 Complex Interacts with the Papillomavirus E2 Protein and Influences Maintenance of Viral Episomal DNA
... replication centers. Replication protein A (RPA) is among the numerous components of the DDR that have been previously shown to be recruited to viral replication foci (32–35). It is required for the repair of ...
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Suppression of cellular proliferation by the papillomavirus E2 protein.
... of E2 expression. To study the downstream effects of E2 expression in HPV-positive cells, we used a conditional BPV-1 E2 mutant that has normal replica- tion and transactivation functions at 32 8 C ...
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Association of Bovine Papillomavirus E2 Protein with Nuclear Structures In Vivo
... of E2 transcription-deficient mutant R37A (2) in this ...wild-type E2 (wtE2) or E2 mutants R37A and E39A and were subjected to digestion with micrococcal nuclease and subsequent extraction with ...
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The Papillomavirus E2 Protein Binds to and Synergizes with C/EBP Factors Involved in Keratinocyte Differentiation
... HPV8 E2, C/EBP factors, and the C/EBP-specific DNA binding ...of E2 and C/EBP factors in vitro and in ...whether E2 was recruited by C/EBP factors bound to DNA, GST-HPV8 E2 or GST immo- ...
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Effect of Bovine Papillomavirus E2 Protein-Specific Monoclonal Antibodies on Papillomavirus DNA Replication
... laboratories. E2 proteins con- taining large internal in-frame deletions of the hinge region (from amino acids 195 to 309, 213 to 309, and 220 to 309) were not able to support DNA replication (or had a decreased ...
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Dimerization of the Papillomavirus E2 Protein Is Required for Efficient Mitotic Chromosome Association and Brd4 Binding
... of E2 interaction surfaces and thereby increase Brd4 ...an E2-Brd4 het- erotetramer, a recent crystallographic structure of the HPV-16 E2 transactivation domain in a complex with a C-terminal ...
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E2 of cottontail rabbit papillomavirus is a nuclear phosphoprotein translated from an mRNA encoding multiple open reading frames.
... We have identified and characterized the cottontail rabbit papillomavirus E2 protein expressed from the late simian virus 40 promoter in COS-7 cells.. E2 was shown to be a highly phospho[r] ...
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Characterization of the Functional Activities of the Bovine Papillomavirus Type 1 E2 Protein Single-Chain Heterodimers
... Three E2 proteins encoded by the BPV1 genome are able to form homo- and heterodimers through their common DBD (10, 19), resulting in the formation of the mixture of E2 homo- and heterodimers, which are all ...
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Adeno-Associated Virus Type 2 Rep Protein Inhibits Human Papillomavirus Type 16 E2 Recruitment of the Transcriptional Coactivator p300
... another protein which inter- acts with the same domain of 16E2, the cellular AMF-1 factor (9), could not be displaced from 16E2 on beads by the same concentrations of ...
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Dynamic Localization of the Human Papillomavirus Type 11 Origin Binding Protein E2 through Mitosis While in Association with the Spindle Apparatus
... cellular protein with ...BPV-1 E2 protein, Brd4 colocalizes with E2 and forms distinct foci on the mitotic chromosomes (4, ...HPV-11 E2 protein relative to Brd4 through mitosis, ...
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