Parainfluenza virus-5
Full Conversion of the Hemagglutinin-Neuraminidase Specificity of the Parainfluenza Virus 5 Fusion Protein by Replacement of 21 Amino Acids in Its Head Region with Those of the Simian Virus 41 Fusion Protein
... most parainfluenza viruses, a virus type-specific interaction between the hemagglutinin-neuraminidase (HN) and fusion (F) pro- teins is a prerequisite for mediating virus-cell fusion and cell-cell ...
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Regulation of Viral RNA Synthesis by the V Protein of Parainfluenza Virus 5
... of parainfluenza virus 5 (PIV5), a proto- typical paramyxovirus, encodes a V protein that inhibits viral RNA ...Nipah virus (NiV), human parainfluenza virus 3 (HPIV3), measles ...
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Sumoylation of the P Protein at K254 Plays an Important Role in Growth of Parainfluenza Virus 5
... of parainfluenza virus 5 (PIV5) is an essential cofactor of the viral RNA-dependent RNA ...a virus that grew to a lower titer and had lower levels of viral RNA synthesis and protein expression ...
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Efficacy of Parainfluenza Virus 5 Mutants Expressing Hemagglutinin from H5N1 Influenza A Virus in Mice
... Construction of plasmids containing full-length PIV5 genomes and HA. To generate a plasmid containing H5-HA insertion between the HN and L genes in the PIV5 ⌬ SH genome (PIV5 ⌬ SH-H5), the plasmid pPIV5 ⌬ SH containing ...
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A Role for Caveolin 1 in Assembly and Budding of the Paramyxovirus Parainfluenza Virus 5
... ecules (e.g., growth factor receptors, src-like kinases, G proteins, and H-Ras) (45, 73). Cav-1 regulates caveola-depen- dent functions, and interaction of cytoplasmic proteins with Cav-1 was shown to be sufficient for ...
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Structure of the Paramyxovirus Parainfluenza Virus 5 Nucleoprotein in Complex with an Amino-Terminal Peptide of the Phosphoprotein
... at 18°C. The purification of each of the chimeric proteins was done from 1 liter of bacterial culture. The pelleted bacterial cells were resuspended in lysis buffer (50 mM Tris buffer, pH 7.6, 1 M NaCl, 0.4 g/ml ...
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A Novel Rabies Vaccine Based on a Recombinant Parainfluenza Virus 5 Expressing Rabies Virus Glycoprotein
... fluenza virus after immunization with PIV5 expressing HA of in- fluenza A virus, demonstrating that a PIV5-based vaccine is effec- tive in dogs with prior exposure ...
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AKT1-Dependent Activation of NF-κB by the L Protein of Parainfluenza Virus 5
... of 5⫻ transfer buffer (60 g of Tris [Trizma base], 285 g of glycine), 800 ml of methyl alcohol, and ...in 5% milk–phosphate-buffered saline containing ...in 5% milk-PBST (Cell Signaling Technology), ...
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Deep sequencing analysis of defective genomes of parainfluenza virus 5 and their role in interferon induction
... restricting virus replication and spread prior to activation of the adaptive immune ...to virus infection, and this leads to the establish- ment of an antiviral state in the infected cell and neighboring ...
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Role of Ubiquitin in Parainfluenza Virus 5 Particle Formation
... Identification of M protein lysine residues targeted for ubiq- uitin attachment. To define primary sites of ubiquitin attachment to the PIV5 M protein, 293T cells were transfected to produce M-HS together with HA-UB. ...
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Analysis of the pH Requirement for Membrane Fusion of Different Isolates of the Paramyxovirus Parainfluenza Virus 5
... influenza virus HA (A/ Udorn/72) have been described previously ...SER virus-in- ...influenza virus at an MOI of 10 ...influenza virus HA. At 16 h p.t., BSR T7/5 cells expressing the T7 ...
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Deep Sequencing Analysis of Defective Genomes of Parainfluenza Virus 5 and Their Role in Interferon Induction
... mumps virus, and influenza A virus), only a small minority of infected cells ever expressed GFP, indicating “heterocellular” activation of the IFN-  promoter ...mumps virus or PIV5-V ⌬ C rich in DIs ...
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The Paramyxovirus Fusion Protein C-Terminal Region: Mutagenesis Indicates an Indivisible Protein Unit
... of parainfluenza virus 5 F (PIV5 F) and either the MPER, the TM domain, or the cytoplasmic tail of the F proteins of the paramyxoviruses measles virus, mumps virus, Newcastle disease ...
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Manipulation of both virus- and cell-specific factors is required for robust transient replication of a hepatitis C virus genotype 3a sub-genomic replicon
... S52 5¢UTR-NS5A with JFH-1- derived NS5B and 3 ¢ UTR (5-5A recombinant), A1672S is one of the three key mutations required for efficient replica- tion, the others being F1464L in NS3 and D2979G in NS5B (the ...
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Prevalence of Viral Severe Acute Respiratory Infections in Patients in the Intensive Care Unit and Those under Mechanical Ventilation in Moi Teaching and Referral Hospital, Uasin Gishu County, Kenya
... Influenza virus causes seasonal epidemics, especially during the winter months with an average annual mortality rate of only 1.4 to 16.7 deaths per 100 000 persons. Increased mortality occur in adults >65 years ...
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The p127 Subunit (DDB1) of the UV-DNA Damage Repair Binding Protein Is Essential for the Targeted Degradation of STAT1 by the V Protein of the Paramyxovirus Simian Virus 5
... controlling virus infections can be judged by the fact that viruses unable to at least partially circumvent the IFN response are attenuated in vivo, and transgenic mice unable to respond to IFN are highly ...
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Analysis of a Viral Agent Isolated from Multiple Sclerosis Brain Tissue: Characterization as a Parainfluenzavirus Type 1
... Using egg-grown 6/94 virus and specific reference sera prepared in guinea pigs against Sendai virus, HA-2, parainfluenza types 2, 3, 4a and 4b, SV5, canine parainfluenza, Newcastle Disea[r] ...
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The regulation of type I interferon production by paramyxoviruses
... ed virus is a poor inducer of type I IFN, and that a good induction profi le only emerges when signifi cant lev- els of defective interfering (DI) particles accumulate within the virus stock preparations ...
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The P gene of human parainfluenza virus type 1 encodes P and C proteins but not a cysteine-rich V protein.
... The P genes of simian virus 5 27, measles virus 2, Sendai virus SEN 29, human parainfluenza virus type 2 PIV2 21, 26, and mumps virus 8 were found to produce more than one mRNA species w[r] ...
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Genetic variability of measles virus during propagation in cultured cells
... measles virus may spread among vaccinated as well as unvaccinated individuals without causing major symptoms (Chen et ...vaccinia virus and adenovirus presenting different structural proteins o f measles ...
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