Particle Assembly and Maturation
Identification of a Structural Element in HIV 1 Gag Required for Virus Particle Assembly and Maturation
... particles, we substituted alanine at each position in the PPIP motif and characterized the mutant viruses by a combination of virological, biochemical, and structural ap- proaches. Substitutions at two positions, P122 ...
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A Single Maturation Cleavage Site in Adenovirus Impacts Cell Entry and Capsid Assembly
... binding, particle internalization, endosome escape, subcellular trafficking, and nuclear translocation of the viral ...for particle assembly and ...delayed particle production, suggesting that ...
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Genome Assembly and Particle Maturation of the Birnavirus Infectious Pancreatic Necrosis Virus
... promotes particle assembly, immediately nucleating capsid components until the generation of ...the particle and the acquisition of infectivity to yield icosahedral virions ...
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Ty3 Nucleocapsid Controls Localization of Particle Assembly
... Expression of the budding yeast retrotransposon Ty3 results in production of viruslike particles (VLPs) and retrotransposition. The Ty3 major structural protein, Gag3, similar to retrovirus Gag, is processed into capsid, ...
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Rescue of Maturation Off-Pathway Products in the Assembly of Pseudomonas Phage ϕ6
... During particle assembly, a P4 hexamer is incorporated at each, or at a majority, of the 5-fold symmetry positions of the P1 shell ...per particle in the particle populations originating from ...
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Cellular Determinants of Hepatitis C Virus Assembly, Maturation, Degradation, and Secretion
... a maturation process that enables them to acquire the low-density configuration prior to leaving the cell ...the assembly and secretion of VLDL and HCV, in that intracellular high-density precursors are ...
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HIV 1 Assembly, Release and Maturation
... include assembly of structural precursors, budding of the new particle and maturation into fully infectious ...appropriate assembly of structural components, the efficient re- cruitment of ...
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A Particle-Associated Glycoprotein Signal Peptide Essential for Virus Maturation and Infectivity
... viral assembly and budding, such as membrane targeting of the capsid, anal- ogously to the matrix subunit of other ...the particle-associated SP cleavage prod- ucts have additional functions in the FV ...
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Study of herpes simplex virus maturation during a synchronous wave of assembly.
... (HSV) particle requires sequential progression of maturing virions through a series of complex assembly ...capsid assembly, we have developed conditions in which an accumulated population of immature ...
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Double-stranded RNA Bacteriophage phi6 : Self-assembly and Maturation of the Procapsid
... vitro assembly reactions to probe potential PC binding ...initial particle formation, a large excess of P4 in the self-assembly reaction slowed the rate of PC self-assembly, which may be ...
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The Maturation Process of pVP2 Requires Assembly of Infectious Bursal Disease Virus Capsids
... DISCUSSION We have discovered a morphogenesis switch controlling the assembly of VLPs in vivo. When expressed in baculovirus, the IBDA polyprotein pVP2-VP4-VP3 is not able to induce the self-assembly of the ...
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Amino acid substitutions in the poliovirus maturation cleavage site affect assembly and result in accumulation of provirions.
... of assembly into empty-capsid-like structures were detected at early times postcoinfection; if the chase period was extended for an additional 12 h, smaller amounts of the empty capsid-like structures were ...
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Assembly-dependent maturation cleavage in provirions of a small icosahedral insect ribovirus.
... Extracts from nodavirus-infected Drosophila cells contained detergent-labile 140S "young" particles much richer than mature virions in their content of protein alpha, a precursor[r] ...
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Analysis of the assembly and maturation processes of the Infectious Bursal Disease Virus capsid
... as assembly, genome packaging, binding to receptors, and uncoating among ...the assembly of the VP2 trimers that constitute the capsid building ...the assembly of IBDV virus-like ...the ...
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Functional Redundancy in HIV-1 Viral Particle Assembly
... It seemed possible that the three elements tested here might cooperate with each other to produce high local Gag concentra- tions within the cell. To explore this possibility, we also tested combinations of the mutations ...
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A domain of the hepadnavirus capsid protein is specifically required for DNA maturation and virus assembly.
... This "amplification" of cccDNA is necessary for high levels of virus production (29), but is under strict control of the large viral envelope protein (P36 in the duck hepatitis B[r] ...
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Assembly of viral membranes: maturation of the vesicular stomatitis virus glycoprotein in the presence of tunicamycin.
... Assem- bly of vesicular stomatitis virus glycoprotein and matrix protein into Hela cell plasma membranes. Transla- tion and identification of the viral mRNA species iso- lated from subce[r] ...
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Coronavirus Particle Assembly: Primary Structure Requirements of the Membrane Protein
... virion assembly or the relative or absolute levels of viral protein expression, that render the whole virus more tolerant to mutational changes that are dev- astating in the VLP ...
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Design colloidal particle morphology and self- assembly for coating applications
... efficiently they thicken. HEC can effectively increase mid- and low-shear viscosity, but are less efficient in increasing high shear viscosity. Paint thickened solely by HEC may suffer from poor flow leveling and spatter ...
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Dissipative Particle Dynamics Simulation of Self-Assembly in a Bolaamphiphilic Solution
... The self-assembly of flexible bolaamphiphilic molecules in a solution is studied by dissipative particle dy- namics simulations. In particular, we investigate the effect of the interaction difference, Δ a, ...
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