PBS, phosphate buffered saline
Development and characterization of magnetic iron oxide nanoparticles with a cisplatin-bearing polymer coating for targeted drug delivery
... Notes: The figures show the mean values of triplicates with standard deviations. Statistical significance in comparison with the untreated control was investigated using the student’s t-test in excel (Microsoft ...
18
Prolonged Scar-in-a-Jar: an in vitro screening tool for anti-fibrotic therapies using biomarkers of extracellular matrix synthesis
... rapamycin; PBS: Phosphate-buffered saline; PDGF: Platelet-derived growth factor; PI3K: Phosphatidylinositol 3- kinase; PRO-C1: ELISA assessing type I collagen formation; PRO-C3: ELISA ...
14
Expression and secretion of fungal endoglucanase II and chimeric cellobiohydrolase I in the oleaginous yeast Lipomyces starkeyi
... CBP: consolidated bioprocessing; E: amplification efficiency; EG II: endo- glucanase II; gal1 : galactokinase gene; GH: glycoside hydrolase; PBS: phosphate-buffered saline; pyk : pyru[r] ...
16
Circulating Magnetic Microbubbles for Localized Real-Time Control of Drug Delivery by Ultrasonography-Guided Magnetic Targeting and Ultrasound
... Figure 1. Fabrication of magnetic microbubbles. (A) a. Scheme of the two-step assembly of magnetic microbubbles – step I: preparation of protamine-functionalized microbubbles; step II: coupling of ...
17
High-dose dexamethasone induced LPS-stimulated rat alveolar macrophages apoptosis
... ice-cold phosphate- buffered saline (PBS; Thermo Fisher Scientific, Waltham, MA, USA) solution by cannulating the trachea with a short- ened 14-gauge needle attached to a 5-mL syringe, which ...
8
Folate-modified Annonaceous acetogenins nanosuspensions and their improved antitumor efficacy
... To determine the dissolution kinetics, 4 mL of FA-ACGs- NSps containing 4.0 mg of ACGs were placed in Float-A- Lyzer dialysis cassettes (molecular weight cutoff 12–14 kDa; Spectrum Labs) and immersed in 2 L of 0.1 mol/L ...
15
Chitosan-coated poly(lactic-co-glycolic) acid nanoparticles as an efficient delivery system for Newcastle disease virus DNA vaccine
... with phosphate-buffered saline (PBS), blocked with 5% fat-free milk overnight, and then incubated with an NDV-positive serum at a 1:500 dilution for 1 ...with PBS with Tween ® ...
11
Respiratory syncytial virus infection in anti-mu-treated mice.
... in 0.05 ml of phosphate-buffered saline PBS intraperitoneally delivered through a 30-gauge needle and was maintained with 50 ,ug intraperitoneally every other day until a schedule Downlo[r] ...
7
Transplantation of cryopreserved human umbilical cord blood-derived endothelial progenitor cells induces recovery of carotid artery injury in nude rats
... with phosphate-buffered saline (PBS, Sigma) and suspended at 5 × 10 7 cells/mL with a final concentration of 10% dimethyl sulfoxide (DMSO) (Sigma) and 20% fetal bovine serum (FBS) ...
12
Cigarette smoke exposure facilitates allergic sensitization in mice
... with phosphate buffered saline (PBS) or OVA (Grade III; Sigma Chemical ...in PBS) were performed using an ultrasonic aerosol gener- ator for 7 min, 4 times a day (Sirius Nova, Heyer ...
9
Annexin A2 binds to vimentin and contributes to porcine reproductive and respiratory syndrome virus multiplication
... PAMs were harvested from 4-week old PRRSV-negative piglets in sterilized phosphate-buffered saline (PBS) by alveolar lavage and then maintained in RPMI-1640 medium. The animal experiments in ...
12
Comparative analysis of syndromic and PCR-based diagnostic assay reveals misdiagnosis/ overtreatment for trichomoniasis based on subjective judgment in symptomatic patients
... in Phosphate Buffered Saline (PBS, 1 mL) for 10 min at 4 °C, mixed by vortexing thoroughly to disperse the sample and ...of PBS followed by centrifugation at 11 000 × g at 4 °C for 5 ...
10
Clinical Translation and First In-Human Use of [44Sc]Sc-PSMA-617 for PET Imaging of Metastasized Castrate-Resistant Prostate Cancer
... p.i.; phosphate buffered saline: PBS; personal computer: PC; positron emission tomography: PET; prostate specific antigen: PSA; prostate specific membrane antigen: PSMA; prostate specific ...
11
Attenuation of nicotine evoked Ca2+ influx by antibody to the nicotinic acetylcholine receptor α3 subunits in human embryonic kidney cells
... in phosphate-buffered saline (PBS), blocked in PBS containing 1% BSA and ...in PBS con- taining 0.3% Triton X-100 (PBS-T), incubated with sec- ondary antibodies (Table 1) ...
6
Rheological and Tribological Properties of Complex Biopolymer Solutions.
... These components, hyaluronic acid (HA) and the plasma proteins, albumin and γ - globulins are combined in a phosphate buffered saline solution (PBS) and subjected to steady shear rheol[r] ...
240
Lipolysaccharide Induced Neuroinflammation Is Associated with Alzheimer Like Amyloidogenic Axonal Pathology and Dendritic Degeneration in Rats
... of phosphate-buffered saline, PBS), the pattern of BACE1 immunoreactivity is com- parable between the ipsilateral and contralateral cortex and hippocampal formation, which is generally ...
17
Nuclear transport of human immunodeficiency virus type 1, visna virus, and equine infectious anemia virus Rev proteins: identification of a family of transferable nuclear export signals.
... After being harvested, cells were resuspended in phosphate-buffered saline PBS supplemented with 250 mM phenylmethylsulfonyl fluoride, 2 mg of aprotinin per ml, and 2 mg of leupeptin per[r] ...
10
Analysis of Immunoprecipitated Surface Glycoproteins in Measles Virions and in Membranes of Infected Cells
... Vero cells in roller bottles were washed with Dulbecco phosphate-buffered saline, pH 7.2 PBS, and inoculated with measles virus LEC strain in minimal essential medium without serum at 29[r] ...
8
Stability and infectivity of novel pandemic influenza A (H1N1) virus in blood derived matrices under different storage conditions
... in PBS, plasma, or buffy ...in PBS, buffy coats, or plasma maintained for up to 3 days storage (at room temperature) or up to 35 days storage (at 4 C) was relatively stable, and showed no significant ...
6
Na⁺ regulation in the intraerythrocytic malaria parasite
... glucose-free saline (in which the glucose was replace isosmotically with NMDG+; light trace) or standard saline with 2 mM NaF (an inhibitor of several glycolytic enzymes; dark ...
226