PCC 6803
The alternative sigma factor SigF is a key player in the control of secretion mechanisms in Synechocystis sp. PCC 6803.
... sp. PCC 6803 physiology, with a major impact on growth and secretion mechanisms, such as the production of extracellular polysaccharides, vesiculation and protein ...
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Increased biomass production and glycogen accumulation in apcE gene deleted Synechocystis sp. PCC 6803
... glucose-tolerant strain of Synechocystis sp. PCC 6803 (21.25 ± 0.55 h and 24.88 ± 0.81 h for GT and ΔapcE mutant, respectively), between 3 to 6 days of culture. These doubling times are similar to that ...
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Identification and Characterization of Enzymes Involved in Post-translational Modifications of Phycobiliproteins in the Cyanobacterium Synechocystis sp. PCC 6803
... All PCR amplification reactions were performed with 40 pmol of each primer, 10 ng of Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002 chromosomal DNA, and 25 µl of PCR Master Mix. All PCR ...
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The Allosteric Role of the AAA(+) Domain of ChlD Protein from the Magnesium Chelatase of Synechocystis Species PCC 6803
... We have produced a series of mutants in the AAA ⫹ domain of ChlD from Synechocystis sp. PCC 6803. These are K49A in the Walker A motif, E152Q in the Walker B motif, R208A removing the arginine finger and ...
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Proteomic analysis reveals resistance mechanism against biofuel hexane in Synechocystis sp. PCC 6803
... sp. PCC 6803, we recently found that five putative transporters involved in transporting of different sub- strates ...sp. PCC 6803, the slr0040, slr0041, slr0043, and slr0044 genes, forming an ...
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Metabolomic basis of laboratory evolution of butanol tolerance in photosynthetic Synechocystis sp. PCC 6803
... sp. PCC 6803 (hereafter Synechocystis) to butanol was found to be at least 10 times lower than other microbes ever being investigated, including Escherichia coli, Zymo- monas mobilis, ...
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Adaptive laboratory evolution of cadmium tolerance in Synechocystis sp. PCC 6803
... rently, the tolerance to cadmium is very low in cyanobacteria. To further engineer cyanobacteria for the environmen- tal application, it is thus necessary to determine the mechanism that they respond to high ...
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Integrated OMICS guided engineering of biofuel butanol-tolerance in photosynthetic Synechocystissp. PCC 6803
... sp. PCC 6803 (hereafter Synechocystis) to bu- tanol using an iTRAQ - LC-MS/MS based proteomics, the results identified 303 proteins differentially regulated by butanol ...
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Expanding the toolbox for Synechocystis sp PCC 6803 : validation of replicative vectors and characterization of a novel set of promoters
... sp. PCC 6803 (hereafter Synechocystis) was the first photosynthetic organism to have its genome sequenced (2) and rapidly became the model strain for ...
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Cloning and expression of two genes (NHAS2 AND NHAS4) isolated from SYNECHOCYSTIS SP PCC 6803 in yeast
... Synechocystis PCC 6803 was PCR amplified with gene-specific primers for the two different Na + /H + antiporter genes (nhaS2 and nhaS4) (Fig ...sp. PCC 6803 by PCR using KpnI site-containing ...
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Synthesis of Chlorophyll-Binding Proteins in a Fully Segregated Δycf54 Strain of the Cyanobacterium Synechocystis PCC 6803
... The Ycf54 protein (12.1 kDa) has been shown recently to interact with the AcsF homolog Sll1214 (hereafter CycI; Peter et al., 2009) in the cyanobacterium Synechocystis PCC 6803 (hereafter Synechocystis; ...
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Insights into isoprene production using the cyanobacterium Synechocystis sp. PCC 6803
... Here, we report on our attempts to establish isoprene synthesis in the model cyanobacterium Synechocystis sp. PCC 6803 (hereafter Synechocystis). In contrast to pre- vious attempts, we used plasmid-based ...
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Enhancement of photosynthetic isobutanol production in engineered cells of Synechocystis PCC 6803
... Synechocystis PCC 6803 and Synechococ- cus elongatus PCC ...Synechocystis PCC 6803, a heterologous expression of α-ketoisovalerate decarboxylase (Kivd) from Lactococcus lactis resulted ...
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Diurnal Regulation of Cellular Processes in the Cyanobacterium Synechocystis sp Strain PCC 6803: Insights from Transcriptomic, Fluxomic, and Physiological Analyses
... Excluding the genes with hypothetical or unknown functions, we focused on how the annotated genes behaved in two consecu- tive L/D cycles. As shown in Table 1, the network whose expres- sion peaks in the light contains a ...
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Butanol tolerance regulated by a two-component response regulator Slr1037 in photosynthetic Synechocystis sp. PCC 6803
... The MS data were processed using Proteome Discoverer software (Version 1.2.0.208) (Thermo Scientific) to gen- erate the peak list. The default parameters of Proteome Discoverer software (Version 1.2.0.208) were used. The ...
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Identification of alcohol stress tolerance genes of Synechocystis sp. PCC 6803 using adaptive laboratory evolution
... constructed using Synechocystis sp. PCC 6803 glucose- tolerant strain as the parental strain by replacing each corresponding gene with a kanamycin-resistance gene. To construct strains in which mutations ...
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Responses of Synechocystis sp. PCC 6803 to heterologous biosynthetic pathways
... Results: In this work, we focused on the production of two different compounds, the cyanogenic glucoside dhurrin and the diterpenoid 13R-manoyl oxide in Synechocystis PCC 6803. We used genome-scale ...
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Ethylene production with engineered Synechocystis sp PCC 6803 strains
... sp PCC 6803 and shows stable ethylene production through the integra- tion of a codon-optimized version of the efe gene under control of the Ptrc promoter and the core Shine–Dalgarno sequence (5′-AGGAGG-3′) ...
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The effect of enhanced acetate influx on Synechocystis sp. PCC 6803 metabolism
... Results: The results show that enhanced acetate intake can efficiently promote the growth of the cyanobacte‑ rial host. The effect is apparent specifically under low‑light conditions when the photosynthetic activity is ...
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A single vector-based strategy for marker-less gene replacement in Synechocystis sp. PCC 6803
... Cyanobacteria, also known as blue-green algae, are Gram-negative, photosynthetic prokaryotes. Moreover, they are the evolutionary ancestors of plastids [1] and the only prokaryotes that exhibit a classical circadian ...
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