PCR amplification, primers and sequencing
Molecular diagnosis of bacterial endocarditis by broad range PCR amplification and direct sequencing
... Most of the above-mentioned studies included the cloning of amplified DNA in a suitable sequencing vector. We have pre- viously simplified this promising molecular method for diag- nostic purposes to include ...
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Blocking primers to enhance PCR amplification of rare sequences in mixed samples – a case study on prey DNA in Antarctic krill stomachs
... a PCR mixture. Even though more sophisticated sequencing techniques such as pyrose- quencing [46] and polony sequencing [47] now open new possibilities in assessing sequence diversity of mixed tem- ...
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Blocking primers to enhance PCR amplification of rare sequences in mixed samples – a case study on prey DNA in Antarctic krill stomachs
... a PCR mixture. Even though more sophisticated sequencing techniques such as pyrose- quencing [46] and polony sequencing [47] now open new possibilities in assessing sequence diversity of mixed tem- ...
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A general method for nested RT PCR amplification and sequencing the complete HCV genotype 1 open reading frame
... nested PCR We optimized nested PCR conditions for each amplicon ...designed primers for nested ...anti-sense primers for each amplicon as shown in Figure ...anti-sense primers must ...
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COLD-PCR Amplification of Bisulfite-Converted DNA Allows the Enrichment and Sequencing of Rare Un-Methylated Genomic Regions
... utilizes primers that bind sequence positions that do not contain CpG sites, ...methylation-specific primers, such as MS- PCR or methyl-Light [7,8], as the methylation status of the primer binding ...
Application of PCR amplicon sequencing using a single primer pair in PCR amplification to assess variations in Helicobacter pylori CagA EPIYA tyrosine phosphorylation motifs
... Primers for CagE were designed from H. pylori strain 26695 [GenBank:AE000511] (CagE-M13-sense primer 5’-TGT AAA ACG ACG GCC AGT GGG GGA ATA GGT TGT TTG GT-3’ and CagE-antisense primer 5’- GGA TCA CCC CAT CAT CTA ...
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Polyadenylated Sequencing Primers Enable Complete Readability of PCR Amplicons Analyzed by Dideoxynucleotide Sequencing
... concentrated PCR buffer (with 15 mM magnesium chloride), 200 mM each of deoxynucleotides (Takara, Japan), 300 nM of the appropriate pair of primers (Generi Biotech, Czech Repub- lic), 50 ng of DNA, and one ...
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Detection and Identification of Mycobacteria by Amplification of the Internal Transcribed Spacer Regions with Genus and Species Specific PCR Primers
... the PCR assays used for detecting mycobacteria involve spe- cies-specific primers targeting the 16S rRNA, hsp65, 32-kDa protein genes, or the internal transcribed spacer (ITS) and detect only a single or a ...
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A database of PCR primers for the chloroplast genomes of higher plants
... utilized PCR-amplified chloroplast gene sequences for establishing and verifying ...of primers recommended for this purpose have also expanded in size ...the amplification of the large single copy ...
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PCR & DNA Sequencing. PCR= Polymerase Chain Reaction. PCR applications
... For amplification of sequences from different organisms, or for "evolutionary PCR", one may increase the chances of getting product by designing "degenerate" primers: Degenerate primers= a set ...
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The Effect of Mismatch Primers on the Efficiency of Amplification in Quantitative Polymerase Chain Reactions
... with PCR using 16S rRNA-specific primers, then ran the DNA products on a denaturing ...gene sequencing, such as biases from PCR, discussed ...
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Universal primers for HBV genome DNA amplification across subtypes: a case study for designing more effective viral primers
... genome amplification and clone-sequencing instead of assembling the PCR sequences of several amplified frag- ments of the ...genome amplification. Günther et al. developed a set of ...
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Designing Of Degenerate Primers-Based Polymerase Chain Reaction (PCR) For Amplification Of WD40 Repeat-Containing Proteins Using Local Allignment Search Method
... degenerate PCR. The TD-PCR starts with a low annealing temperature (42°C) for 15 cycles, followed by 20 cycles at a high annealing temperature ...of amplification, the 5'-ends of each primer ...
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Impact of Enzymes and Primers on the PCR Amplification of Some Goatfishes
... of PCR amplification of target genes from genomic DNA (gDNA) of many environmental specimens (Schneider, Enkerli, & Widmer, ...of PCR, but normally inhibit the polymerase from amplifying the ...
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PCR primers Universal primers for PCR am-
... in PCR amplification and transcrip- tion of two PVY isolates in a mixed ...strain-specific primers for the separate amplification of each target sequence in a mixed virus ...
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Supplementary Table 1. Forward and reverse primers used in PCR amplification and cycle
... Bayesian phylogenetic trees estimated with partitioned models of sequence evolution by codons, where first, second and third position were.. estimated separately.[r] ...
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Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries
... enrichment PCR step as the primary source of base-composition bias in frag- ment libraries and developed an optimized PCR proto- col that produces libraries that are far less skewed than standard ...
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Pcr amplification by two different primers for early diagnosis of schistosoma mansoni infected snails
... for PCR is obligatory. In the present study, two types of primers for conventional PCR were used and compare between them and shedding, crushing) in attempt to apply this technique in the field for ...
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Detection of Mycobacterium tuberculosis by PCR amplification with pan Mycobacterium primers and hybridization to an M tuberculosis specific probe
... a PCR assay for the detection of ...quality-controlled amplification and detection reagents to ensure optimal performance and incorporates dUTP and uracil-N-glycosylase to effectively control false-pos- ...
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Amplification/PCR. Amplification Software 303
... real-time PCR, and a bright red formulation, easily visible when recovering reaction products (not recommended for sealing Microseal ® 384-well PCR ...
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