PCR based Enzyme immunoassay:
Semiquantitative Species Specific Detection of Bartonella henselae and Bartonella quintana by PCR Enzyme Immunoassay
... by PCR and to improve the species differentiation, we developed a semiquantitative, species-specific PCR-based enzyme immunoassay ...Biotin-labeled PCR products were immobilized ...
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Rapid Detection of Chlamydia pneumoniae by PCR Enzyme Immunoassay
... by PCR, an enzyme immunoassay (EIA) for analysis of PCR products was ...Biotin-labeled PCR products generated from the 16S rRNA gene of ...Bound PCR product-probe hybrids were ...
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Detection of Tritrichomonas foetus by PCR and DNA Enzyme Immunoassay Based on rRNA Gene Unit Sequences
... a PCR test optimized for applicability in routine ...is based upon primers TFR3 and TFR4 directed to the rRNA gene units of ...DNA enzyme immunoassay for the detection of amplification ...
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Comparison of Enzyme Immunoassay and Reverse Transcriptase PCR for Identification of Serotype G9 Rotaviruses
... RT- PCR genotyping were also G9 by EIA, thus confirming that serotype G9 rotaviruses were a common cause of rotavirus infections of neonates in India in ...
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Detection of Campylobacter in Stool and Determination of Significance by Culture, Enzyme Immunoassay, and PCR in Developing Countries
... In this work, we compared the results of three diagnostic modal- ities to detect Campylobacter and documented substantial under- detection by selective culture in comparison to the levels of detec- tion by both EIA and ...
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Aspergillus Galactomannan Enzyme Immunoassay and Quantitative PCR for Diagnosis of Invasive Aspergillosis with Bronchoalveolar Lavage Fluid
... One antigen-based assay is the GM double-sandwich EIA. This assay has been recently approved by the U.S. Food and Drug Administration as an adjunctive test for IPA diagnosis when applied to serum. With this ...
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Microtitration plate enzyme immunoassay to detect PCR amplified DNA from Candida species in blood
... described PCR amplification of Candida ...nested PCR techniques (which add to the time required to perform PCR-based tests and to the risk of sample contamination due to additional sample ...
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Comparison of the Clinical Performance of PapilloCheck Human Papillomavirus Detection with That of the GP5+/6+ PCR Enzyme Immunoassay in Population Based Cervical Screening
... Overall, there was good genotyping agreement between the two assays, although the PapilloCheck assay seemed to detect more HPV type 31, 51, 52, and 59 infections, particularly in individuals with multiple infections. ...
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Rapid Differentiation of Aspergillus Species from Other Medically Important Opportunistic Molds and Yeasts by PCR Enzyme Immunoassay
... resolved based on its much-reduced (10-fold lower) A 650 value compared to that for ...the PCR-EIA probe matrix could be used in clinical laboratories as a rapid and simple screening test for the most ...
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Loop Mediated Isothermal Amplification Compared to Real Time PCR and Enzyme Immunoassay for Toxigenic Clostridium difficile Detection
... Department of Clinical Pathology, Beaumont Hospitals, Royal Oak, Michigan, USA a ; Oakland University William Beaumont School of Medicine, Rochester, Michigan, USA b ; Division of Infectious Diseases, Beaumont Hospitals, ...
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Monoclonal Antibody Based Enzyme Immunoassay for Epidemiological Studies of Asymptomatic Dengue Infection
... The diagnostic criteria for recent dengue infection using paired annual serum sample may be different from the criteria used for acute dengue infection using acute and convalescent sera because the rise in IgG level may ...
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Comparison of enzyme immunoassay, PCR, and type specific cDNA probe techniques for identification of group A rotavirus gene 4 types (P types)
... Hybridization and chemiluminescence detection were carried out as described previously (9). RESULTS The sensitivity and specificity of each assay were calculated by reference to a “gold standard” whereby the correct P ...
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Enzyme linked immunoassay for detection of PCR amplified DNA of legionellae in bronchoalveolar fluid
... an immunoassay was developed without loss of ...of PCR products labeled with biotin requires high biotin- binding capacity of the microtiter plates, since 10 m l of the PCR reaction mixture contains ...
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ENZYME IMMUNOASSAY KIT
... GENERAL PRINCIPLE OF ENZYME IMMUNOASSAY The immunoplate in this kit is pre-coated with a secondary antibody, whose nonspecifi c binding sites are blocked. The secondary antibody can bind to the Fc fragment ...
Use of a nested PCR enzyme immunoassay with an internal control to detect Chlamydophila psittaciin turkeys
... nested PCR-EIA detected 31 additional positives, resulting in a total of 105 ...105 PCR-EIA positives were initially negative by the EIA and during retesting, when the internal control was added to the ...
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Reverse transcription PCR detection of LaCrosse virus in mosquitoes and comparison with enzyme immunoassay and virus isolation
... A reverse transcription-PCR (RT-PCR) assay was developed and compared with enzyme immunoassay (EIA) and virus isolation for detecting LaCrosse virus (LAC) in mosquito pools.. All three t[r] ...
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Clinically practical seminested PCR for Burkholderia pseudomallei quantitated by enzyme immunoassay with and without solution hybridization
... seminested PCR protocols combined with enzyme immunoassay (EIA) to detect the conserved ribosomal regulatory region of ...capturing PCR products on EIA ...hybridized PCR products with a ...
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Detection of poliovirus antigen by enzyme immunoassay
... In conclusion, owing to the small amount of poliovirus in feces, the EIA is not suitable for the diagnosis of poliovirus infections directly from clinical specimens, but it can be used t[r] ...
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Use of PCR enzyme immunoassay for identification of influenza A virus matrix RNA in clinical samples negative for cultivable virus
... The evaluation of respiratory samples from human volunteers infected with influenza A virus under controlled conditions allowed us to examine the relative sensitivities of PCR-EIA and vi[r] ...
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Monoclonal antibody based enzyme immunoassay for Giardia lamblia antigen in human stool
... A visually readable monoclonal antibody-based antigen-capture enzyme immunoassay for the detection of Giardia lamblia antigen in human stool specimens was developed and found to be 97% ([r] ...
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