PCR Primers for infection detection
PCR primers Universal primers for PCR am-
... When PCR products of mixed PVY isolates served as templates for transcription, RNA synthesis was also in- ...strain-specific primers in the separate amplification of each target sequence in the ...
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Specific Primers for Rapid Detection of Microsporum audouinii by PCR in Clinical Samples
... These initial molecular approaches chiefly showed that der- matophytes, in general, were fundamentally closely related or- ganisms. Nonetheless, characterization by microscopic examination and in vitro culture of tinea ...
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Evaluation of PCR Primers for Early Diagnosis of Cytomegalovirus Infection following Liver Transplantation
... by PCR had high levels of sensitivity and specificity for the detection of CMV infection and a high degree of sensitivity but a lower degree of specificity for the detection of symptomatic CMV ...
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Development of PCR Assays with Nested Primers Specific for Differential Detection of Three Human Anelloviruses and Early Acquisition of Dual or Triple Infection during Infancy
... cies-specific primers derived from a highly conserved area lo- cated just downstream of the TATA box of the TTV, TTMDV, and TTMV genomes, we developed a new PCR assay method to separately detect the genomic ...
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Optimization of PCR-ELISA in Detection of Human Cytomegalovirus Infection
... Methods: PCR–ELISA was performed by using specific primers and probe for detection of the HCMV glycoprotein B ...DIG-labeled PCR products. The probe-PCR product hybrid is immobilized on ...
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Comparison of PCR methods for detection of Leishmania siamensis infection
... of primers amplifying the tim gene has been designed which could amplify Leishmania at the concen- tration as low as ...the PCR method amplifying the cytb gene also showed a low sensi- tivity at ...two ...
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PCR in Diagnosis of Infection: Detection of Bacteria in Cerebrospinal Fluids
... several PCR protocols to enhance sensitivity, especially when dealing with small numbers of bac- teria as the ...Nested PCR is one of these protocols for detection of only a few bacteria in clinical ...
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Use of PGMY Primers in L1 Consensus PCR Improves Detection of Human Papillomavirus DNA in Genital Samples
... for detection and typing with PCR of human papillomavirus (HPV) DNA in genital ...PGMY primers for the detection and typing of HPV by comparing the results obtained with PGMY09/PGMY11 and ...
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PCR mediated detection of sex and PaLCuV infection in papaya - A review
... of primers poses some ...multiplex PCR applications include pathogen identification, linkage analysis, gender screening, forensic studies, genetic disease diagnosis and quantitative analysis of nucleic acid ...
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Developing the role of PCR for detection and characterisation of helicobacters in human infection
... selecting primers with similar annealing temperatures (based on length, and G + C content), and the possibility o f primer dimer formation should be minimised also by avoiding sequence homologies (Elnifro et a l ...
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PCR-Based Diagnosis of Neoscytalidium dimidiatum Infection Using Internal Transcribed Spacer 1 Region of Ribosomal DNA Primers
... feet infection who attended Dermatology clinic Siriraj Hospital during October 2015 to November 2015 were used for PCR ...dimidiatum-specific PCR primers, the PCR product was amplified ...
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Panfungal PCR Assay for Detection of Fungal Infection in Human Blood Specimens
... panfungal PCR assay which detects the small-subunit rRNA gene sequence of the two major fungal organism groups was used to test whole-blood specimens obtained from a series of blood or bone marrow transplant ...
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Comparison of Multiplex PCR-ELISA and conventional Multiplex PCR for detection of HIV-1/HCV co-infection
... Probe and Primer design. For performance, target RNA sequences were selected within the regions of the respective virus genomes showing maximum sequence conservation for the ability of the test to de- tect all known ...
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Detection of sexually transmitted infection and human papillomavirus in negative cytology by multiplex-PCR
... sets. PCR reactions (20 μL) were performed with 2 μL tem- plate DNA, 1 μM of each primer, and 2x Master mix (Seegene, ...Korea). PCR amplification was carried out with an Applied Biosystem 9700 thermal ...
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Highly specific and efficient primers for in house multiplex PCR detection of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma hominis and Ureaplasma urealyticum
... Traditionally, testing for C. trachomatis and N. gonor- rhoeae is performed on cervical swabs that are sent for culture, phenotypic or immunological detection [7]. These tests are often not performed immediately, ...
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Detection of Mycobacterium ulcerans by real-time PCR with improved primers
... results, PCR sensitivity was more signifi- cantly improved by new primers for three Japanese iso- ...conventional primers than other ...of detection sensitivity, while for most Japanese ...
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Detection of Mycobacterium leprae infection by PCR
... 1994 PCR amplification of the 531-bp fragment of the Mycobacterium leprae pra gene in fresh biopsy and slit skin smear samples was evaluated for its usefulness in the detection of leprosy bacilli in ...
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PCR-based detection of Plasmodium falciparum in saliva using mitochondrial cox3 and varATS primers
... gene-based PCR frequently amplified non-specific bands ...cox3 primers target a gene specific to Plasmo- dium , thereby eliminating the risk of non-specific amp- lification of human ...VarATS PCR ...
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Development of a Universal Intimin Antiserum and PCR Primers
... the PCR primers synthesized to generate Int 388–667 gave a specific PCR product with as the template eae-positive strains belonging to different EPEC and EHEC serogroups isolated from a variety of ...
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COMPUTATION AND IN SILICO VALIDATION OF CANDIDATE PRIMERS FOR PCR FREE ON SITE DETECTION OF ENTEROTOXIGENIC ESCHERICHIA COLI
... on-site detection of ETEC is important for the management of these ...potential detection tool to diagnose the causative ...candidate primers of isothermal amplification to detect ETEC. The ...
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