PCR-Restriction Fragment Length Polymorphism analysis
Identification of 54 Mycobacterial Species by PCR Restriction Fragment Length Polymorphism Analysis of the hsp65Gene
... of restriction fragments as deduced by sequence analysis and allowed identification of mycobacteria whose PRA pat- terns were unequivocally identified by fragments shorter than 60 ...yielded fragment ...
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Identification of Staphylococcus spp by PCR Restriction Fragment Length Polymorphism Analysis of dnaJ Gene
... hand, PCR-RFLP analysis is useful for the taxonomy of staphylococci and proves to be easier, less expen- sive, and less equipment dependent than ...Recently, PCR-RFLP analysis of the internal ...
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Molecular typing of Borrelia burgdorferi sensu lato by PCR restriction fragment length polymorphism analysis
... burgdorferi sensu lato are responsible for distinctive clinical manifestations of Lyme disease. Elucidation of the relationship between species and disease is complicated by the difficulty in culturing B. burgdorferi ...
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Routine use of PCR restriction fragment length polymorphism analysis for identification of mycobacteria growing in liquid media
... a PCR capability was to adopt a PCR-based procedure that would allow us to identify acid-fast isolates growing in BACTEC 12B and 13A ...other PCR-based procedures capable of identifying multiple ...
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Rapid Identification and Typing ofStaphylococcus aureus by PCR Restriction Fragment Length Polymorphism Analysis of the aroA Gene
... the analysis could be performed by a simple method that is able to identify all potentially related strains, after which a second method capable of discriminating among individual isolates could be ...Our ...
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PCR Restriction Fragment Length Polymorphism Analysis for Identification of Bacteroides spp and Characterization of Nitroimidazole Resistance Genes
... HpaII PCR-RFLP profiles consistent with nimB, but the TaqI PCR-RFLP profiles were inconsistent with reported nim genes (Table 2 and ...gene PCR products indicated that these strains contained a novel ...
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Identification of Oral Peptostreptococcus Isolates by PCR Restriction Fragment Length Polymorphism Analysis of 16S rRNA Genes
... this PCR-RFLP method as an alternative and more accurate method than phenotypic meth- ods for species-level identification of clinical isolates tenta- tively identified by culture methods as Peptostreptococcus ...
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Molecular Identification of Capnocytophaga spp via 16S rRNA PCR Restriction Fragment Length Polymorphism Analysis
... rRNA PCR-RFLP (36), and 16S rRNA sequence analysis (6, ...rRNA PCR-RFLP) are labor-intensive, costly, and time-consuming and therefore not suitable for most microbiology laboratories, especially when ...
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Recognition of a Nocardia transvalensis complex by resistance to aminoglycosides, including amikacin, and PCR restriction fragment length polymorphism analysis
... 2. PCR-restriction fragment length polymorphism (RFLP) analysis of a 439-bp segment of the 65-kDa heat shock protein gene with XhoI and HinfI produced identical patterns for 53 ...
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Comparison of PCR Restriction Fragment Length Polymorphism Analysis and PCR Direct Sequencing Methods for Differentiating Helicobacter pylori ureB Gene Variants
... utilizing PCR-restriction fragment length polymorphism (RFLP) in the Helicobacter pylori genes is widely used to differentiate ...specific restriction site can be ...the ...
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Differentiation of Burkholderia Species by PCR Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene and Application to Cystic Fibrosis Isolates
... the PCR-RFLP method has been applied to a large variety of presumed ...by PCR ribotyping allowed a selection of genetically unrelated strains to assess the diversity within this bacterial ...the ...
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Detection of Amantadine Resistant Influenza A Virus Strains in Nursing Homes by PCR Restriction Fragment Length Polymorphism Analysis with Nasopharyngeal Swabs
... The PCR-RFLP genotyping method reported by Klimov et ...a PCR-RFLP analysis to distinguish resistant vi- ruses, focusing on amino acid substitutions at positions 27, 30, and 31, using inexpensive and ...
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Species Identification of Mycobacteria by PCR Restriction Fragment Length Polymorphism of therpoB Gene
... 2000 PCR-restriction fragment length polymorphism analysis (PRA) using the novel region of the rpoB gene was developed for rapid and precise identification of mycobacteria to the ...
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Rapid identification of Campylobacter species by restriction fragment length polymorphism analysis of a PCR amplified fragment of the gene coding for 16S rRNA
... Restriction fragment length polymorphism analysis of a PCR-amplified DNA fragment of the gene coding for 16S rRNA was performed on 148 previously characterized strains of ...
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Sensitive PCR Restriction Fragment Length Polymorphism Assay for Detection and Genotyping of Giardia duodenalis in Human Feces
... heminested PCR-restriction fragment length polymorphism analysis for the detection and genotyping of Giardia duodenalis on the basis of polymorphism in the triose ...
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Evaluation of PCR Restriction Profile Analysis and IS2404 Restriction Fragment Length Polymorphism and Amplified Fragment Length Polymorphism Fingerprinting for Identification and Typing of Mycobacterium ulcerans andM marinum
... comparative analysis including PCR restriction profile analysis (PRPA), IS2404 restriction fragment length polymorphism (RFLP), and amplified fragment ...
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Genetic Identification of the Main Opportunistic Mucorales by PCR Restriction Fragment Length Polymorphism
... A PCR strategy selectively amplifies genomic DNA from molds belonging to the genera Absidia, Mucor, Rhizopus, and Rhizomucor, excluding human DNA and DNA from other filamentous fungi and ...
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Identification of Infectious Agents in Onychomycoses by PCR Terminal Restriction Fragment Length Polymorphism
... decade, PCR assays have been developed for the direct detection of fungi in nail ...a PCR-terminal restriction fragment length polymorphism (TRFLP) assay to directly and ...
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Detection of Cryptosporidium and Identification to the Species Level by Nested PCR and Restriction Fragment Length Polymorphism
... nested PCR and restriction fragment length polymorphism (RFLP) that offers simple identification of Cryptosporidium hominis, Cryptosporidium parvum, and most other human infective ...
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Restriction fragment length polymorphism analysis of clinical isolates of Mycobacterium haemophilum
... The identical RFLP patterns observed among different isolates that were collected over many years (16 years in the case of the type 2 clone) and from the same patient (patient 12) over 3[r] ...
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