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Peptide and protein analysis

Protein identification: the origins of peptide mass fingerprinting

Protein identification: the origins of peptide mass fingerprinting

... Peptide mass fingerprinting (PMF) grew from a need for a faster, more efficient method to identify frequently observed proteins in electrophoresis gels. We describe the genesis of the idea in 1989, and show the ...

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Mass spectrometry analysis of protein/peptide S-palmitoylation

Mass spectrometry analysis of protein/peptide S-palmitoylation

... energy for the auto-acylation process could be significantly lower than that for enzymatic transacylation, and suggested that enzymatic palmitoylation may not play an important role in vivo. This hypothesis was ...

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Analysis of protein RNA and protein peptide interactions in Equine Infectious Anemia Virus (EIAV) infection

Analysis of protein RNA and protein peptide interactions in Equine Infectious Anemia Virus (EIAV) infection

... for protein structure prediction, including ab initio and fold recognition methods capable of incorporating predicted protein- protein contacts as ...

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Analysis of whey protein hydrolysates: peptide profile and ACE inhibitory activity

Analysis of whey protein hydrolysates: peptide profile and ACE inhibitory activity

... whey protein concentrate with a nutritionally adequate peptide profile and the ability to inhibit angiotensin-converting enzyme (ACE) ...the peptide profile and the ACE-IA. The best peptide ...

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Peptide vocabulary analysis reveals ultra conservation and homonymity in protein sequences

Peptide vocabulary analysis reveals ultra conservation and homonymity in protein sequences

... In summary, within NRL3D_63, longer words are mostly indicative of conservation. Some of them are islands of ultra-conservation within distinctly divergent proteins. However, annotation or Pfam domain mapping indicates ...

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Solid Phase Peptide Synthesis: Analysis and Identification of Protein Kinase Substrates

Solid Phase Peptide Synthesis: Analysis and Identification of Protein Kinase Substrates

... This research study examines the synthesis of tyrosine containing peptides based on the protein paxillin. Paxillin is a proposed substrate of the Focal Adhesion Kinase (FAK) protein tyrosine kinase. FAK is ...

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Protein-protein and peptide-protein docking and refinement using ATTRACT in CAPRI

Protein-protein and peptide-protein docking and refinement using ATTRACT in CAPRI

... from peptide-protein interactions to docking of large proteins to the ...of peptide-protein docking (pepATTRACT) [37] and of refinement (iATTRACT) [38] within the last ...flexibility ...

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Analysis of the relationship between cleavability of a paramyxovirus fusion protein and length of the connecting peptide.

Analysis of the relationship between cleavability of a paramyxovirus fusion protein and length of the connecting peptide.

... The results presented here suggest that in the paramyxovirus F protein the number of basic amino acid residues in the connecting peptide is important for cleavage of the precursor protei[r] ...

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Mutational Analysis of the Fusion Peptide of Moloney Murine Leukemia Virus Transmembrane Protein p15E

Mutational Analysis of the Fusion Peptide of Moloney Murine Leukemia Virus Transmembrane Protein p15E

... envelope protein, p15E, contains a hydrophobic stretch of amino acids at its N terminus followed by a region rich in glycine and threonine ...envelope protein was ...envelope protein to cause ...

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Rotavirus neutralizing protein VP7: antigenic determinants investigated by sequence analysis and peptide synthesis.

Rotavirus neutralizing protein VP7: antigenic determinants investigated by sequence analysis and peptide synthesis.

... Although the variable hydrophilic regions of VP7 were obvious candidates to account for the antigenic differences between serotypes, peptide antisera which clearly bound homologous pepti[r] ...

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Sequence Analysis and Modeling of Antimicrobial Peptide from Goat Milk Protein Hydrolyzed by Bromelain

Sequence Analysis and Modeling of Antimicrobial Peptide from Goat Milk Protein Hydrolyzed by Bromelain

... milk protein by bromelain generates fractions <10 kDa which act as ...Sequence analysis and modeling of the peptides sequences are important to predict their potential ...modeling. Analysis of the ...

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BALB/c myeloma retroviruses: peptide mapping and immunological analysis of the pp12 structural protein.

BALB/c myeloma retroviruses: peptide mapping and immunological analysis of the pp12 structural protein.

... the ppi2 proteins of the two myeloma viruses only shared partial antigenic identity with the ppl2 of this amphotropic virus. Thus, the im-[r] ...

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The Structural Basis of Peptide-Protein Binding Strategies

The Structural Basis of Peptide-Protein Binding Strategies

... flexible peptide to a rigid, well-defined bound structure? A structure-based analysis of peptide-protein interactions unravels that most peptides do not induce conformational changes on their ...

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Aquasomes -The Best Carriers for Protein and Peptide Delivery

Aquasomes -The Best Carriers for Protein and Peptide Delivery

... FT-IR spectroscopy can be used for structural analysis. Using KBr sample disk method, the core as well as the coated core can be analyzed by recording their IR spectra in the range of 4000–400 cm–1; the ...

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Integrative Analysis Frameworks for Improved Peptide and Protein Identifications from Tandem Mass Spectrometry Data.

Integrative Analysis Frameworks for Improved Peptide and Protein Identifications from Tandem Mass Spectrometry Data.

... eschewed protein-level FDR altogether, preferring to utilize varying levels of peptide-level FDR filtering to control error ...such peptide-level filtering provides an inaccurate measure of ...

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Evaluating Peptide Mass Fingerprinting-based Protein Identification

Evaluating Peptide Mass Fingerprinting-based Protein Identification

... for protein identification is that: at what point does one use MS/MS analysis for protein identification? A com- monly used approach is to use PMF as an initial step, and then use MS/MS to ...

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The success of protein and peptide therapeutics is

The success of protein and peptide therapeutics is

... Surmodics’ low-shear microencapsulation process technology and formulation compositions can maintain protein integrity. Stability and release kinetics can be improved with proprietary microencapsulation technology ...

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Amino acid, Peptide, Protein

Amino acid, Peptide, Protein

... o Lower blood glucose level.. Mice with balsam pear peptide show reduced blood glucose level.. BALSAM PEAR PEPTIDE B) Hypolipidemic activity. o Decrease cholesterol and fatty acid synth[r] ...

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Designing helical peptide inhibitors of protein–protein interactions

Designing helical peptide inhibitors of protein–protein interactions

... short peptide to bind to a specific target-protein interaction surface poses a difficult ...helical peptide inhibitors of protein-protein ...landmark protein targets of high ...

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Nanoparticles in relation to peptide and protein aggregation

Nanoparticles in relation to peptide and protein aggregation

... and peptide and protein aggregation The size of nanoparticles is one of the key parameters that influence the interaction between nanoparticles and ...proteins. Protein structure, function, and their ...

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