peptide library
Peptide Mimotopes Selected from a Random Peptide Library for Diagnosis of Epstein Barr Virus Infection
... Epstein-Barr virus (EBV) is a ubiquitous, worldwide infectious agent that causes infectious mononucleosis, affecting >90% of the world’s population. Currently, enzyme-linked immunosorbent assay, mostly with purified ...
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Identification Of Dengue-Specific B-Cell Epitopes By Phage-Display Random Peptide Library
... random peptide libraries (RPL) displayed on phage has provided a powerful tool for selecting sequences that mimic epitopes from microorganisms that are useful for diagnostic and vaccine development ...
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Induction of anti-EGFR immune response with mimotopes identified from a phage display peptide library by panitumumab
... display peptide library with ...display peptide library could be promising candidate vaccines for active anti- EGFR immunotherapy against ...
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Screening and selection of peptides specific for esophageal cancer cells from a phage display peptide library
... The Ph.D.-12 TM Phage Display Peptide Library and E. coli ER2738 were purchased from New England Biolabs (Beverly, MA, USA). The Eca109 cell line and human esophageal can- cer tissue sections were obtained ...
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Development of a Gallium-Containing One-Bead One-Compound Peptide Library for the Discovery of New Molecular Imaging Probes
... (OBOC) peptide library, containing a gallium-DOTA complex, was developed in an attempt to overcome common issues associated with the later addition of radionuclides to peptide chains of imaging ...a ...
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A mimotope from a solid-phase peptide library induces a measles virus-neutralizing and protective antibody response.
... combinatorial peptide library was used to generate a panel of mimotopes of an epitope recognized by a monoclonal antibody to the F protein of measles virus ...epitope peptide (from MV fusion ...
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Screening and Identification of a Targeting Peptide to Hepatocarcinoma from a Phage Display Peptide Library
... phage peptide library; peptide HCBP1 (FQHPSFI) and nonspecific control peptide (AFSIKQW) were synthesized and labeled with fluorescein isothio- cyanate (FITC) by Shanghai Bioengi- neering Ltd; ...
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Mimotopes of the Vi Antigen of Salmonella enterica Serovar Typhi Identified from Phage Display Peptide Library
... phage-displayed peptide library was used to identify mimotopes (epitope analogues) of this antigen by panning against a ViCPS-specific monoclonal antibody (MAb) ...the peptide sequence TSHHDSHGLHRV, ...
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Screening and identification of a renal carcinoma specific peptide from a phage display peptide library
... synthetic peptide (0, ...synthetic peptide corresponding to an unrelated phage picked randomly from the original phage peptide library) were used as negative ...
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Identification of a conserved JEV serocomplex B cell epitope by screening a phage display peptide library with a mAb generated against West Nile virus capsid protein
... the peptide library with 6D3. We next sequenced the peptide insert of the ten selected phage clones that reacted with the 6D3 ...the peptide insert sequences indicated that six 6D3-reac- tive ...
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Use of a Sec signal peptide library from Bacillus subtilis for the optimization of cutinase secretion in Corynebacterium glutamicum
... This workflow, beginning with preculturing from a single colony after library transformation needs to be validated. Hence, a mixture of C. glutamicum cells har- boring different expression plasmids as encountered ...
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Generation and Characterization of Monoclonal Antibodies against Dengue Virus Type 1 for Epitope Mapping and Serological Detection by Epitope-Based Peptide Antigens
... random peptide library displayed on ...Epitope-based peptide antigens were proved able to detect antibodies in serum samples collected from DEN-1-infected patients but not in those taken from ...
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A high throughput method for identifying personalized tumor- associated antigens
... random peptide library screening, we identified patient-specific sets of peptides recognized by colon cancer patients’ serum IgG and IgM ...random peptide library screening on cancer patient’s ...
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Evolution of Cyclic Peptide Scaffolds to Target Nucleic Acids
... The Progress Report assay completed after Round 3 of the second attempt at selection along with the Round 3 amplified phage titer indicated that the evolved phage pool had significant wildtype contamination. Wildtype M13 ...
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A Peptide Mimic of a Protective Epitope of Respiratory Syncytial Virus Selected from a Combinatorial Library Induces Virus-Neutralizing Antibodies and Reduces Viral Load In Vivo
... solid-phase peptide library with MAb 19 (Table 1), and Swiss-Prot analysis showed that the sequences have no homology with the primary amino acid sequence of RSV F ...a peptide-based ELISA (Table 1), ...
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Development of a Peptide Mediated Capture PCR for Detection of Mycobacterium avium subsp paratuberculosis in Milk
... In order to solve the problem of standardization, we propose the use of high-affinity peptides as an alternative to antibodies in routine diagnostic microbiology. In this study we used a commercially available ...
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Selection of Muscle-Binding Peptides from Context-Specific Peptide-Presenting Phage Libraries for Adenoviral Vector Targeting
... “context-specific” peptide-presenting phage ...random peptide library between the H and I sheets of the fiber protein of adenovirus type 5 on the pIII protein of fd ...This library was used ...
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ACPA fine specificity profiles in early rheumatoid arthritis patients do not correlate with clinical features at baseline or with disease progression
... citrullinated peptide 2 (CCP2) reactivity is shown: anti-CCP2-positive is shown in different shades of brown and orange, and anti-CCP2-negative is shown in ...Lib.: peptide library; MNDA: myeloid ...
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Peptides That Bind Specifically to an Antibody from a Chronic Lymphocytic Leukemia Clone Expressing Unmutated Immunoglobulin Variable Region Genes
... Phage Library Sorting and Analysis Preparation of the pVIII peptide library was reported previously (21). Wells of Costar EIA/RIA high-binding plates (Corning, Big Flats, NY, USA) were coated with ...
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Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library
... the peptide substrates for Hsp40-Ydj1, we took advantage of a commercially available M13 phage peptide display library from New England ...phage peptide display library screen has been ...
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