Perforin (−/−) mice
Perforin independent β cell destruction by diabetogenic CD8+ T lymphocytes in transgenic nonobese diabetic mice
... unknown, perforin-linked Idd ...if perforin is the major mechanism of β-cell cyto- toxicity, why do perforin-competent ...NOD.lpr/lpr mice not develop diabetes? The lack of ...
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Theiler’s Virus Infection of Perforin-Deficient Mice
... Based on the study of several viruses such as LCMV, VSV, vaccinia virus, and SFV, it has been hypothesized that noncyto- pathic viruses are controlled mainly by specific lytic mechanisms, whereas cytopathic viruses are ...
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Thymic Tolerance to Only One Viral Protein Reduces Lymphocytic Choriomeningitis Virus-Induced Immunopathology and Increases Survival in Perforin-Deficient Mice
... in perforin-deficient mice after LCMV infection and increases their long-term ...of perforin-deficient thymic expressors and nonexpres- ...all perforin-deficient animals died after LCMV ...
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Loss of the Perforin Cytotoxic Pathway Predisposes Mice to Experimental Cytomegalovirus Retinitis
... the perforin-mediated and receptor- mediated cytotoxic pathway in providing resistance to HCMV retinitis in immunologically normal persons with systemic HCMV infection have not yet been ...the perforin and ...
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Alternative Mechanisms of Respiratory Syncytial Virus Clearance in Perforin Knockout Mice Lead to Enhanced Disease
... in perforin knockout (PKO) mice is slightly delayed but that both PKO and wild-type mice clear virus by day 10, suggesting an alternative mechanism of RSV ...of mice were shown to lyse Fas ...
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Mouse hepatitis virus is cleared from the central nervous systems of mice lacking perforin-mediated cytolysis.
... of perforin-mediated cytotoxicity in acute JHMV CNS infection, the clinical progression of disease and mortality of perforin ( 2 / 2 ) and ( 1 / 1 ) littermates and H-2- compatible C57BL/6 mice ...
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Control of immunodeficiency and lymphoproliferation in mouse AIDS: studies of mice deficient in CD8+ T cells or perforin.
... B10.A mice homozygous for the b 2M mutation and B10.D2 mice homozygous for the perforin mutation were diagnosed as having MAIDS by 5 to 8 weeks after infection by the criteria of lymphoproliferation, ...
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Pathogenesis of Herpes Simplex Virus Type 1-Induced Corneal Inflammation in Perforin-Deficient Mice
... whether perforin-dependent cytotoxicity is an important effector mechanism in the production of ...and perforin-deficient (PKO) mice were infected intracorneally with HSV-1 strain ...PKO mice ...
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Lack of both Fas Ligand and Perforin Protects from Flavivirus-Mediated Encephalitis in Mice
... into mice is strongly host factor dependent (for a review, see reference ...32); mice up to 3 to 4 weeks of age are highly susceptible to a low-dose virus inoculum, whereas in older animals the peripheral ...
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Upregulation of the Genes Encoding Lysosomal Hydrolases, a Perforin-Like Protein, and Peroxidases in the Brains of Mice Affected with an Experimental Prion Disease
... solution for 30 min at room temperature to abolish endogenous peroxidase activity. After treatment with normal rabbit serum, the tissue sections were allowed to react overnight at 4°C with anti-GFAP (1:50 [Dako]) and ...
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Granzymes and Caspase 3 Play Important Roles in Control of Gammaherpesvirus Latency
... ⫺/⫺ mice likely reflects the in vitro observations that while GzmB is required for rapid induction of apoptosis (26, 63), its absence can be compen- sated for by the activity of GzmA ...in perforin ⫺/⫺ ...
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CD8+ T Lymphocytes Mediate Borna Disease Virus-Induced Immunopathology Independently of Perforin
... of mice as determined by flow cytometric analysis (data not ...from perforin 0/0 mice ...⫹/⫹ mice efficiently lysed p40-expressing L929 target cells ...or perforin 0/0 brain lymphocyte ...
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Mice Deficient in Perforin, CD4+ T Cells, or CD28-Mediated Signaling Maintain the Typical Immunodominance Hierarchies of CD8+ T-Cell Responses to Influenza Virus
... John Harty (43). Eight- to 10-week-old female mice were primed by intraperi- toneal (i.p.) injection with 600 hemagglutinating units of influenza virus A/Puerto Rico/8/34 (PR8). For blocking experiments, ...
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CD8+ T Cells Require Perforin To Clear West Nile Virus from Infected Neurons
... of perforin-expressing, cytolytic CD8 ⫹ T cells has a detrimental effect after infection with a virulent lineage I New York WNV ...when perforin-deficient mice were infected with the lineage II ...
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Role of perforin in controlling B cell hyperactivity and humoral autoimmunity
... B6pfp→F1 mice exhibit persistent survival of host B cells and autoantibody ...wt→F1 mice is long lived and is still observed at 9–12 weeks after parental cell transfer (Figure ...GVHD mice (Table 1). ...
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Myonuclear apoptosis in dystrophic mdx muscle occurs by perforin mediated cytotoxicity
... mdx mice, histologically discernible muscle cell death does not ap- pear at the onset of muscle ...mdx mice, and DMD hu- mans display mild, histologically discernible muscle pathology at birth but remain ...
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Molecular study of the perforin gene in familial hematological malignancies
... Therefore, mice lacking peforin are profoundly immunodeficient and have an enhanced sus- ceptibility to viral infection and ...with perforin deficiency is concei- vably due to a defective cytotoxic ...
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Perforin deficiency attenuates collagen induced arthritis
... Fas-deficient mice devel- oped arthritis that was less severe, probably through a reduced IL-1R1/Toll-like receptor-4 signaling that might con- tribute to a decreased expression of other cytokines, chemok- ines ...
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Modulatory effects of perforin gene dosage on pathogen-associated blood-brain barrier (BBB) disruption
... that perforin is a key regulator of BBB disruption is ...the perforin allele has extensive single nucleotide variants (SNVs) ...Clinically, perforin SNVs are viewed unfavor- ably, as loss of ...
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Critical Role for Perforin-, Fas/FasL-, and TNFR1-Mediated Cytotoxic Pathways in Down-Regulation of Antigen-Specific T Cells during Persistent Viral Infection
... B6 mice in comparison to those of FasL (gld) mice. B6 control mice (A and B) or FasL (gld) mutant mice (C and D) were infected with 2 ⫻ 10 6 or 10 2 PFU of LCMV Docile as ...
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