PFGE typing
Original Article Application of pulsed-field gel electrophoresis (PFGE) in Bacillus cereus typing
... used typing methods for ...results. Typing results can be affected by different personnel or pathogens’ physiology ...[6]. PFGE typing rec- ognizes large molecular weight DNA fragments with ...
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Emergence of New CMRSA7/USA400 Methicillin Resistant Staphylococcus aureus spa Types in Alberta, Canada, from 2005 to 2012
... Methicillin-resistant Staphylococcus aureus (MRSA) has become one of the most significant pathogens affecting global public health and health care systems. In Canada and the United States, the spread of MRSA is primarily ...
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Sparrows, flies, and rodents as reservoirs of Campylobacter spp on a dairy farm : a thesis presented in partial fulfilment of the requirements for the degree of Master of Veterinary Science in Veterinary Public Health and Meat Hygiene at Massey University, Palmerston North, New Zealand
... The PFGE typing yielded seven, six, nine, six and three restriction patterns from dairy cows, farm sparrows, urban sparrows, rodents and flies respectively.. PFGE analysis of the C.[r] ...
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Comparison of Restriction Enzymes for Pulsed Field Gel Electrophoresis Typing of Moraxella catarrhalis
... (PFGE) typing methodologies have been reported (9–13), with NotI as the most prevalent enzyme and the best choice for genotyping ...molecular typing study performed on ...
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Comparison of an Automated Repetitive Sequence Based PCR Microbial Typing System with Pulsed Field Gel Electrophoresis for Molecular Typing of Vancomycin Resistant Enterococcus faecium
... molecular typing of ...whereas PFGE showed polyclonal ...whereas PFGE detected six ...with PFGE typing but was less discriminative than PFGE in defining clonal ...to PFGE ...
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Use of Pulsed Field Gel Electrophoresis for Molecular Epidemiologic and Population Genetic Studies ofMycobacterium tuberculosis
... by PFGE typing using each of four ...of PFGE patterns on different images enabled us to cluster identical ...independent PFGE en- TABLE 2. Discriminatory abilities of PFGE and ...
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Analysis of Typing Methods for Epidemiological Surveillance of both Methicillin Resistant and Methicillin Susceptible Staphylococcus aureus Strains
... for typing Staphylococcus aureus, such as multilocus sequence typing (MLST) and spa typing, have increased interlaboratory reproducibility, portability, and speed in obtaining results, but ...
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High Rate of Tetracycline Resistance inStreptococcus pyogenes in Iran: an Epidemiological Study
... PFGE typing of a number of nontypeable strains from Teh- ran, for all of which the tetracycline MIC was 64 g/liter, was attempted since it was possible that these strains could belong to a single clone or ...
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... Characteristics of the patients included in this investiga- tion, were showed in the Table .: diagnosis, days in hos- pital, date of admission and discharge, and the type of iso- lated strain MRSA. Th e strain of the ...
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Multilocus Sequence Typing for Characterization of Clinical and Environmental Salmonella Strains
... improved discriminatory ability of MLST can be useful for the differentiation of strains involved in food-borne outbreaks of salmonellosis, including strains within the same serotype that are strongly clonal and that may ...
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International Multicenter Evaluation of the DiversiLab Bacterial Typing System for Escherichia coli and Klebsiella spp
... by PFGE were selected from the collections of the Hospital Hygiene Department of the University Medical Center Utrecht and a study on the population distribution of  -lactamases conferring resistance to broad- ...
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Comparison of the DiversiLab Repetitive Element PCR System with spa Typing and Pulsed Field Gel Electrophoresis for Clonal Characterization of Methicillin Resistant Staphylococcus aureus
... ever, typing methods have also become an indispensable tool for distinguishing clonal nosocomial clusters from unrelated increase of MRSA ...genetic typing methods for the clonal characterization of ...
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Proof of Principle for Successful Characterization of Methicillin Resistant Coagulase Negative Staphylococci Isolated from Skin by Use of Raman Spectroscopy and Pulsed Field Gel Electrophoresis
... PFGE. Isolates were cultured on Columbia blood agar plates and incubated overnight at 35°C. PFGE was performed as described previously (4). Briefly, a suspension of bacteria was mixed with 1% InCert agarose ...
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SmaI Restriction Site Based Multiplex PCR for Typing of Hospital and Community Acquired Staphylococcus aureus
... Strains and growth conditions. Well-characterized strains of S. aureus and Staphylococcus epidermidis strains were obtained from various sources. Se- quenced S. aureus strains CA-629 (NRS745), MRSA252 (Sanger 252/NRS71), ...
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Serogroup C meningococci in Italy in the era of conjugate menC vaccination
... sequence typing), porA VRs (Variable Region) typing, PFGE (Pulsed Field Gel Electrophoresis), VNTR (Variable Number Tandem Repeats) analyses were performed on all C:2a and C:2b meningococci (n = ...
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Investigation of Kingella kingae Invasive Infection Outbreaks in Day Care Facilities: Assessment of a Rapid Genotyping Tool Targeting the DNA Uptake Sequence
... DNA uptake sequence typing. DUST was performed for all the strains in the Parisian center, as previously described (10). Briefly, DNA PCR amplification was performed with the primer king3DUS (5=-AAGCAGCCTGCA-3=) ...
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Patterns of Resistance Associated with Integrons, the Extended Spectrum β Lactamase SHV 5 Gene, and a Multidrug Efflux Pump of Klebsiella pneumoniae Causing a Nosocomial Outbreak
... Macrorestriction analysis of DNA by PFGE. A representative set of the pre- sumed K. pneumoniae outbreak isolates was characterized by pulsed-field gel electrophoresis (PFGE). Two sets of nine Klebsiella ...
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Clonality and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus at the University Hospital Zurich, Switzerland between 2012 and 2014
... by PFGE since 1992 within the scope of our local isolation ...the PFGE data with an additional typing method (spa-typing), and to determine which of the globally known clones were found at our ...
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Evaluation of Phenotypic and Genotypic Methods for Subtyping Campylobacter jejuni Isolates from Humans, Poultry, and Cattle
... for typing of clonal population, whereas stable and perhaps less discriminatory methods are necessary for typing panmictic populations in order to determine the correct relations between ...All ...
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Genotyping of Methicillin Resistant Staphylococcus aureus Strains from Two Hospitals in Bangalore, South India
... Molecular typing methods have in the last few years paved the way for sophisticated techniques to track the source and transmission route of bacterial pathogens in hospital outbreaks and have also helped in ...
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