PH domain
Identification of a PH domain containing protein which is localized to crystalloid bodies of Plasmodium ookinetes
... and PH domain (PY17X_0705100, des- ignated as PyCryPH-p; ...a PH domain is expressed in zygotes/ookinetes and localizes to the surface membrane (PY17X_0420000) ...the PH domain ...
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Design and synthesis of chemical probes for the protein kinase B PH domain
... Improved selectivity for PKB has been achieved in the synthesis of allosteric inhibitors (Figure 1.12). 121,122 These are in fact PKBα and PKBβ isoform selective and highly selective against closely related kinases such ...
203
Multiscale Simulations Suggest a Mechanism for the Association of the Dok7 PH Domain with PIP-Containing Membranes
... (PH) domain and a Phosphotyrosine Binding (PTB) ...Dok7 PH/membrane complex. Our simulations indicate that the PH domain of Dok7 associates with membranes contain- ing ...
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MYO1C Binds to PiP2 With High Affinity Through a Putative Ph Domain
... tail domain binds tightly and specifically to PIP2 in a non-cooperative ...characterized PH domains inhibit myo1c binding to PIP2 in vitro and eradicate correct localization and membrane binding in ...
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Real time fluorescence imaging of PLC gamma translocation and its interaction with the epidermal growth factor receptor
... the PH domain (PHacPLC␥2 and PHalaPLC ␥ 2), demonstrated the same calcium responses as seen with the wild-type PLC␥2 ...isolated PH domain, which has been suggested in previous studies using ...
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OCDD: an obesity and co-morbid disease database
... with PH domain and leucine zipper 1; CASP: Caspases, or cysteine-aspartic proteases or cysteine-dependent aspartate-directed proteases; ER: Endoplasmic reticulum; FFA: Free fatty acids; FA: Fatty acids; ...
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Recombinant CTRP9 administration attenuates neuroinflammation via activating adiponectin receptor 1 after intracerebral hemorrhage in mice
... Western blotting was performed as described previously [20]. Briefly, the brain protein samples were prepared using Ripa Lysis buffer (Bio-Rad, CA), and equal amounts of protein were run on an SDS-PAGE gel. After being ...
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Analysis of Epichloë festucae membrane lipid composition and its role in Nox complex assembly : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Genetics at Massey University, Palmerston North, New Zealand
... The lipid-binding PH domain of Nox protein Cdc24 was found to be necessary for membrane localisation, supporting a role for lipid signalling in fungal Nox complex regulation.. To identif[r] ...
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Analysis of Epichloë festucae membrane lipid composition and its role in Nox complex assembly : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Genetics at Massey University, Palmerston North, New Zealand
... as PH and PX ...homology domain (CH) at the N-terminus, potentially involved in actin binding (IPR036872); a pleckstrin homology domain (PH), involved in binding PPIs (IPR033511); and a Phox ...
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The polybasic region of cytohesin 2 determines paxillin binding specificity to mediate cell migration
... same domain structure, which contains the N-terminal CC do- main, the catalytic Sec7 domain, and the PH domain plus the C-terminal basic amino acid extension ...the PH domain in ...
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The characterisation of Plc1 : a phospholipase C enzyme identified in the fission yeast Schizosaccharomyces pombe
... catalytic domain, as the PH domain is able to bind PtdIns 3,4,5-P3Garcia et al., 1995 as statedin section 1.2.2.3,PLCs are unableto hydrolyse Ptdlns 3A5-P3The PH domain is not required f[r] ...
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Functional analysis of non-hotspot AKT1 mutants found in human breast cancers identifies novel driver mutations: implications for personalized medicine
... novel PH domain mutants similarly conferred constitutive membrane targeting of Akt1, we generated expression constructs fusing the PH domains of wild type and mutant Akt1 proteins with enhanced green ...
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Determine The PH Domaine of PLC-gamma in the Egg of Starfish during Fertilization
... the PH-domain of PLCγ will be fused with GFP and this PH- GFP fusion protein will be used for localization studies in the starfish Patiria ...
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Role of Oxysterol Binding Protein in Hepatitis C Virus infection
... teins are implicated in cholesterol homeostasis, phospholipid metabolism, vesicular transport, and cell signaling (55). OSBP functions as sterol sensor that regulates the transport of ceramide from the ER to the Golgi ...
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Wsp1 Is Downstream of Cin1 and Regulates Vesicle Transport and Actin Cytoskeleton as an Effector of Cdc42 and Rac1 in Cryptococcus neoformans
... A domain interacts with the Arp2/3 ...EH domain and the coiled-coil region, ...(WH2) domain, two SRC homol- ogy 3 (SH3) domains, and a C-terminal DH-PH domain ...
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A novel <em>BCR-ABL1</em> mutation in a patient with Philadelphia chromosome-positive B-cell acute lymphoblastic leukemia
... RNA was extracted from bone marrow white blood cells. Briefly, 5 mL of sample was mixed with 10 mL of red blood cell lysis solution (5 mM MgCL 2 , 10 mM NaCl and 10 mM Tris-HCl; pH 7) and centrifuged at 400 × g ...
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Evolution of the eukaryotic ARP2/3 activators of the WASP family: WASP, WAVE, WASH, and WHAMM, and the proposed new family members WAWH and WAML
... WH2 domain, and because this domain is very short (Entamoebae genes rarely contain introns so that we would have expected longer hits if there would be homology to neighbouring regions, which we would ...
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Molecular structure and regulation of vertebrate Na+/H+ exchangers
... N-terminal domain of the exchanger since NHE-1, after removal of the entire cytoplasmic domain, remains sensitive to amiloride (Wakabayashi et ...transmembrane domain of the ...transmembrane ...
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Site-Directed Antibodies against the Stem Region Reveal Low pH-Induced Conformational Changes of the Semliki Forest Virus Fusion Protein
... exogenous domain III proteins containing the stem region showed significantly increased binding to E1 at low pH and a more potent inhibition of virus-membrane fusion (29), suggest- ing that stem ...
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Acidic pH Mediates Changes in Antigenic and Oligomeric Conformation of Herpes Simplex Virus gB and Is a Determinant of Cell-Specific Entry
... High-MW gB ( ⬎ 180 kDa) corresponding to oligomers was detected in cells trans- fected with gB subjected to 1% SDS treatment alone (Fig. 3a). After exposure to pH 5.0, the gB high-MW oligomers were not detected, ...
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