PHA, phytohemagglutinin-P
Mesenchymal stem cells from umbilical cord matrix, adipose tissue and bone marrow exhibit different capability to suppress peripheral blood B, natural killer and T cells
... E2; PHA: Phytohemagglutinin; PKC: Protein kinase C; SSC: Side scatter; STAT3: Signal transducer and activator of transcription 3; TCR: T cell receptor; TGF- β : Transforming growth factor- β ; TLR: Toll ...
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SLC39A8 gene encoding a metal ion transporter: discovery and bench to bedside
... Osteoarthritis; PHA: Phytohemagglutinin; PLCB1: Phospholipase-C-beta-1 gene; RI: Recombinant inbred; rvZIP8: Stably transfected MEFs containing ZIP8 cDNA via retroviral infection; Sglt2 > Cre > ...
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Evaluation of biological safety in vitro and immunogenicity in vivo of recombinant Escherichia coli Shiga toxoids as candidate vaccines in cattle
... Neither incubation of PHA-P (phytohemagglutinin-P) stimulated PBMC with rStx WT nor incubation with rStx mut led to a significant in- crease in the percentage of late apoptotic/necrotic [r] ...
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MHC restricted fratricide of human lymphocytes expressing survivin specific transgenic T cell receptors
... ulation with either phytohemagglutinin PHA or a combination of CD3- and CD28-specific antibodies, activated HLA-A2– lymphocytes were not recognized by effector cells expressing Tg-TCR, e[r] ...
10
Cystathionine Synthase Activity in Human Lymphocytes: Induction by Phytohemagglutinin
... of phytohemagglutinin (PHA) to short-term lymphocyte cultures results in a significant induction of enzymatic ...This PHA- stimulated activity has characteristics that resemble those previously ...
5
Cytokine Responses to Stimulation of Whole Blood from Patients with Buruli Ulcer Disease in Ghana
... with phytohemagglutinin (PHA) and tuberculin purified protein derivative (PPD) of whole blood from 39 (23 early- and 16 late-stage) BUD patients and 39 healthy control subjects in ...
5
Homocystinuria: Heterozygote Detection using Phytohemagglutinin Stimulated Lymphocytes
... Using phytohemagglutinin (PHA)-stimulated lymphocytes as a readily available source of this enzyme, its activity has been compared in 48 control subjects, seven homozygotes affected with homocystinuria, and ...
5
Identification of a New Quaternary Neutralizing Epitope on Human Immunodeficiency Virus Type 1 Virus Particles
... MAb 2909 was also tested for neutralization against the parental form of virus SF162 in the GHOST cell neutralization assay 5 and the phytohemagglutinin PHA-stimulated PBMC assay 14; 50%[r] ...
6
Decreased ex vivo production of interferon gamma is associated with severity and poor prognosis in patients with lupus
... Background: Lupus pathogenesis is closely associated with interferon gamma (IFN- γ ), which plays a central role in innate and adaptive immunity. The aim of this study was to evaluate the ex vivo production of IFN- γ ...
9
<p>Impact of Low Interferon-γ and IL-10 Levels on TNF-α and IL-6 Production by PHA-Induced PBMCs in Type 2 Diabetes Mellitus</p>
... by PHA remains controversial; however, some studies have reported that PHA initiates cell signaling via CD2 and CD3 receptor, which leads to Ca 2+ in fl ux through calcium ...9,10 PHA activated- Th1 ...
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Adenosine metabolism in phytohemagglutinin stimulated human lymphocytes
... The association of a human genetic deficiency of adenosine deaminase activity with combined immunodeficiency prompted a study of the effects of adenosine and of inhibition of adenosine deaminase activity on human ...
14
Bacterial Production of Hydroxyalkanoates (PHA)
... Among these new materials stand out poly-hydroxyalkanoates (PHA), plastic biopolymers produced by bacteria [3]. The biotechnological production of such bacterial plastic materials (bioplastics) is one of the ...
8
ISOLATION, PRODUCTION AND EXTRACTION OF BACTERIAL POLYHYDROXYALKANOATE ENZYME
... of PHA are rubbery and flexibility in nature and have low ...1992). PHA polymer of medium length chain is more potential for application due to increase elasticity and decrease crystallinity as compared to ...
10
Replication of Poliovirus in Phytohemagglutinin-stimulated Human Lymphocytes
... 40 [ W0 DISCUSSION In our study of poliovirus replication in leukocytes, the original observations of Gresser and Chany could be confirmed 10, namely, that nonstimulated leukocyte cultur[r] ...
7
strain 12600 ATCC) and Escherichia coli E.coli strain 8739 ATCC) as compared to bare PHA or PHA-rGO in 2 hours of time. The P value (P< 0.05) was obtained by using 2 sample T-test distribution.
... of PHA, PHA/rGO and PHA/GAg nanocomposite were aliquotted with 5mL of culture broth of each test organism ...with PHA disc alone as a negative ...
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<p>Structure–activity relationship of an antimicrobial peptide, Phylloseptin-PHa: balance of hydrophobicity and charge determines the selectivity of bioactivities</p>
... Background: Antimicrobial peptides (AMPs) from the skin secretions of amphibians are now considered as a potential alternative to conventional antibiotics. Phylloseptins are a family of AMPs identified in the skin ...
12
Production of Medium Chain Length Poly (3 Hydroxyalkanoates) from Saponified Palm Kernel Oil by Pseudomonas putida: Kinetics of Batch and Fed Batch Fermentations
... PHA accumulation by bacteria is a response to the imbalance in growth environment, with excess carbon source and simultaneous limitation of nutrients such as nitrogen, phosphorus, oxygen, magnesium etc; this ...
9
The interaction of human macrophages and lymphocytes in the phytohemagglutinin stimulated production of interferon
... response, phytohemagglutinin was required for the duration of the culture, and both cell types in association were ...from phytohemagglutinin-stimulated macrophages or lymphocytes could not substitute for ...
11
Cellular Immune Responses to Extracellular Streptococcal Products in Rheumatic Heart Disease
... to phytohemagglutinin, and to Candida albicans antigen were measured in tonsillar and peripheral blood lymphocytes from patients with rheumatic heart disease (RHD) and suitably matched nonrheumatic (control) ...
8
The in vitro response of bovine peripheral blood lymphocytes to phytohemagglutinin and tuberculin
... On the basis of several lines of evidence, it is likely that the type of ^ vitro response of bovine lymphocytes to tuberculin FPD is distinct from the response to PK^, and represents ant[r] ...
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