Phenotypic based methods for carbapenem-resistant organisms
Comparison of phenotypic methods for the detection of carbapenem non-susceptible Enterobacteriaceae
... workflow based on phenotypic analysis in the routine detection and characterization of carbapenem resistant Enterobacteria- ceae ...different phenotypic assays: 1) MHT, 2) MBL Etest, 3) ...
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Phenotypic and genotypic characterization of carbapenem-resistant Acinetobacter baumannii isolates from Egypt
... four carbapenem-resistant isolates were screened for carbapenemase production by a number of phenotypic methods (MHT, CIM, CDT, CarbAci- neto NP test and boronic acid disc test) (Table ...the ...
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Phenotypic and molecular characterisations of carbapenem-resistant Acinetobacter baumannii strains isolated in Madagascar
... options. Carbapenem-resistant ...in carbapenem resistance in the Acinetobacter species [7, ...baumannii carbapenem re- sistance [9, ...ISs. Based on the variable domains of this porin, ...
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Phenotypic and genotypic characteristics of carbapenem-resistant Enterobacteriaceae in a tertiary-level reference hospital in Turkey
... Methods: A total of 181 CRE strains were included in the study. Antimicrobial susceptibility rates were tested using Vitek 2 system. Modified Hodge test (MHT) was performed using meropenem and ertapenem discs. ...
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Comparison of Minocycline Susceptibility Testing Methods for Carbapenem Resistant Acinetobacter baumannii
... testing methods having moderately high minor error rates, but the disk diffusion method using MH agar from BD gave the least discordant ...the methods due to the MICs distributing mostly in the ...
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Imputation Methods and Modeling of Environmental Reservoirs of Nosocomial Carbapenem-Resistant Bacteria
... bacteria and further identification of specific organism found. This type of sampling has an estimated 90 percent sensitivity for identifying CRE organisms[4]. Figure 4.1: Bar plot of total samples by unit, Sample ...
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Direct Detection of Carbapenem-Resistant Organisms from Environmental Samples Using the GeneXpert Molecular Diagnostic System
... culture-based methods (3 to 4 days), the cost of the Carba-R assay cartridges is more ...culture-based methods can. The culture method may have an advantage for phenotypic detection of ...
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Phenotypic and molecular characterization of the carbapenem resistant Klebsiella pneumoniae and Escherichia coli causing blood stream infections
... Microarray are DNA hybridization techniques that permit simultaneous detection of many sequences.Ulyashova et al. established a microarray technique which could identify GIM, KPC,OXA, IMP, VIM, SPM and SIM ...
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TO STUDY PHENOTYPIC AND MOLECULAR CHARACTERISTICS OF CARBAPENEM RESISTANT KLEBSIELLA PNEUMONIAE ISOLATED FROM TERTIARY CARE HOSPITAL
... identified based on their morphological, physiological and biochemical characters using microscopic observation, standard biochemical methods and cultural ...
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Environmental Contamination by Carbapenem Resistant Enterobacteriaceae
... of carbapenem resistance in Enterobacteriaceae has posed great concern to public ...of carbapenem-resistant Enterobac- teriaceae (CRE) are currently ...sampling methods, CHROMAgar KPC contact ...
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Active Surveillance of Multidrug-Resistant Organisms with Rapid Detection Methods for Infection Control
... tant organisms (MDROs) such as methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococcus (VRE), and multidrug-resistant Pseudo- monas aeruginosa and Acinetobacter ...
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Biosafety research into Diabrotica resistant Bt-maize: indicator organisms and monitoring methods
... density based on the results from different field sampling ...Non-target organisms suitable for Environmental Risk Assessment including problem formulation and the monitoring of adverse effects of the ...
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Phenotypic and Molecular Characteristics of Carbapenem Resistant Enterobacteriaceae in a Health Care System in Los Angeles, California, from 2011 to 2013
... time of flight, and broth microdilution tests were repeated to confirm the CRE phenotype. Real-time PCR for bla KPC , bla SME , bla IMP , bla NDM-1 , bla VIM , and bla OXA-48 was performed. Clonality was assessed by ...
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Prevalence and Characterization of carbapenem resistant organisms causing urinary tract infections among hospitalized patients and outcomes of these infections in a tertiary care center
... carbapenemase producer along with the identification as CR E.coli, CR Klebsiella species or CRE other than E.coli and Klebsiella species at 24 hours. Comparison of MHT, CarbaNP and SUPERCARBA CHROMagar results: Among the ...
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Principles and Applications of Methods for DNA Based Typing of Microbial Organisms
... sequences of HIV clade B proteases of 167 HIV isolates taken from 102 patients in order to identify mutations associated with resistance to protease inhibitors. More recently, Troesch et al. (77) have described the use ...
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The Problem of Carbapenemase Producing Carbapenem Resistant Enterobacteriaceae Detection
... These methods have the benefit of determining the exact mechanism conferring car- bapenem resistance, which can be especially helpful during out- break investigations and while performing epidemiological re- ...
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Drug Repurposing to Fight Colistin and Carbapenem-Resistant Bacteria
... strains resistant to carbapenem and/or ...multi-drug resistant bacteria (MDR), is ...major methods are described: phenotypic, computational and ...for carbapenem and ...
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Evaluation of carbapenem resistance using phenotypic and genotypic techniques in Enterobacteriaceae isolates
... control methods, create a basis for epidemiological ...other phenotypic methods, antibiotic susceptibility profiles are not ideal because they can be affected by growth medium, phase of reproduction ...
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Evaluation of PCR Based Testing for Surveillance of KPC Producing Carbapenem Resistant Members of the Enterobacteriaceae Family
... Our study has several limitations. First, the lack of a gold standard allowed only an internal comparison of the methods studied, and true sensitivity may therefore be lower than re- ported. Second, we interpreted ...
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Phenotypic Detection of Carbapenemase Producing Organisms from Clinical Isolates
... of carbapenem- resistant Gram-negative clinical isolates ...single phenotypic test that meets all specifications of the “perfect” test, there are a number of options that are user-friendly, accurate, ...
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