Phorbol-12
Geraniol suppresses proinflammatory mediators in phorbol 12-myristate 13-acetate with A23187-induced HMC-1 cells
... Scientific). Phorbol 12-myristate 13-acetate (PMA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazo- lium bromide (MTT), calcium ionophore (A23187), DMSO, and phosphate-buffered saline (PBS) were procured ...
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Nanomechanical measurement of adhesion and migration of leukemia cells with phorbol 12-myristate 13-acetate treatment
... of phorbol 12-myristate 13-acetate (PMA) treatment, the conventional batch-cell transwell-migration assay may not indicate the intrinsic effect of the treatment on migration, since the treatment may also ...
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Inhibition of Osteopontin Synthesis in THP-1 Cells Stimulated with Phorbol 12-Myristate 13-Acetate by Brefelamide Derivatives
... in phorbol 12-myristate 13-acetate (PMA)-stimulated THP-1 cells was confirmed using enzyme- linked immunosorbent assay (ELISA), a multicolor immune-fluorescent microscope and western blot ...
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Protein kinase C activation mediates interferon-β-induced neuronal excitability changes in neocortical pyramidal neurons
... 4β- phorbol 12-myristate 13-acetate (4β-PMA) or Bryostatin1 and PKC inhibitors GF109203X (also known as BisI or Gö6850) or calphostin C (all Tocris Bioscience, Bristol, UK) were dissolved in ...
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Soluble CD40L is associated with increased oxidative burst and neutrophil extracellular trap release in Behçet’s disease
... Hydrogen peroxide and superoxide anion quantification Neutrophils and PBMCs were separated by density gra- dient separation using dextran and Ficoll-Hypaque 1077, respectively. Cells were stimulated with (1) 30 ng/ml ...
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IL-32θ inhibits monocytic differentiation of leukemia cells by attenuating expression of transcription factor PU.1
... PU.1 is a key transcription factor regulating the myeloid differentiation. PU.1- induced monocytic differentiation into macrophage is also important for blood cancer development. Therefore, we chose THP-1 monocytic ...
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Priming of polymorphonuclear neutrophils by atrial natriuretic peptide in vitro
... anion. Phorbol 12-myristate 13-acetate, opsonized zymosan, or FMLP could all be used as triggering stimuli to demonstrate the priming of PMN activation by ...
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Reduced glutathione as a physiological co activator in the activation of peptidylarginine deiminase
... Fig. 4 Release of catalytically active peptidylarginine deiminase ( PAD ) from PMA-stimulated granulocytes. a Microtitre plates coated with human fibrinogen were incubated for 3 h at RT with blood leucocytes resuspended ...
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Possible mechanism of phorbol diester induced maturation of human promyelocytic leukemia cells
... 12,13-dibutyrate. Phorbol diester analogues inhibited [3H]phorbol 12,13-dibutyrate binding in a stereospecific manner consistent with their biologic ...soluble phorbol diester receptors prepared by ...
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Induction of interleukin-6 after stimulation of human B-cell CD21 by Epstein-Barr virus glycoproteins gp350 and gp220.
... Two of the hallmarks of in vitro EBV B-cell infection are continued B-cell growth and increased Ig synthesis. These characteristics are believed to result from the expression of a limited number of virally encoded genes ...
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Pharmacological targeting of <em>β</em>-adrenergic receptor functions abrogates NF-κB signaling and MMP-9 secretion in medulloblastoma cells
... receptors was confirmed in DAOY cells by semiquantitative RT-PCR. We next found that propranolol dose-dependently inhibited induction of the key extracellular matrix-degrading and blood–brain barrier disrupting enzyme ...
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Caffeic acid phenethyl ester modulates Helicobacter pylori induced nuclear factor kappa B and activator protein 1 expression in gastric epithelial cells
... pylori; NF-kB; AP-1; COX-2; gastric epithelial cells AP-1, activator protein-1; CAPE, caffeic acid phenethyl ester; NAC, N-acetylcysteine; NF-kB, nuclear factorkappa B; PMA, phorbol 12-m[r] ...
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Properties and roles of the three subclasses of histamine receptors in brain
... Effect of a phorbol ester 4^-phorbol 12,13-dibutyrate; PDB on the stimulation of cyclic AMP accumulation in slices from guinea-pig hippocampus elicited by activation of histamine Hr and [r] ...
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Original Article PKCδ reveals a tumor promoter function by promoting cell proliferation and migration in somatotropinomas
... Abstract: Protein kinase C δ (PKCδ), a subtype of PKC family, has been recognized as a tumor promoter or suppres- sor depending on different tissue specificities in various tumor types. However, the effects of PKCδ on ...
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Effects of protein kinase C activation on sodium, potassium, chloride, and total CO2 transport in the rabbit cortical collecting tubule
... C, phorbol 12-myristate,13-acetate (PMA) and L-alpha-1,2-dioctanoylglycerol (L-alpha-1,2-DOG), and two inactive analogues, 4 alpha-phorbol and 4-O-methyl phorbol ...
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Low production of reactive oxygen species in granulocytes is associated with organ damage in systemic lupus erythematosus
... Figure 1 Polymorphonuclear leukocytes (PMN) from patients with systemic lupus erythematosus (SLE) produced fewer reactive oxygen species (ROS) than PMN from healthy blood donors. The capacities of PMN from healthy blood ...
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RAZ, an Epstein-Barr virus transdominant repressor that modulates the viral reactivation mechanism.
... Viral reactivation in vitro is produced by treatment of latently infected cell lines with agents such as phorbol esters 12, sodium butyrate 40, or anti-immunoglobulin 11, 55, all of whic[r] ...
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Mouse mammary tumor virus-related sequences in mouse lymphocytes are inducible by 12-O-tetradecanoyl phorbol-13-acetate.
... One set of filters was hybridized to a 32P-labeled cDNA probe prepared with polyA+ membrane or cytoplasmic mRNA of induced cells and the other, to a 32P-labeled cDNA probe prepared with [r] ...
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12 O Tetradecanoyl Phorbol 13 Acetate Release of Glycosyltransferases from Human Blood Cells
... with 12-O-tetradecanoyl-phorbol-13-acetate (TPA), the release of these three glycosyltransferases increased two- to six-fold, and cell suspension glycosyltransferase activities decreased ...
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Expression of the protein product of the mouse mammary tumor virus long terminal repeat gene in phorbol ester-treated mouse T-cell-leukemia cells.
... 441 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Exposure of C57BL/6 mouse EL-4 T-cell leukemia cells to phorbol ester 12-O-tetradecanoylphorbol-13acetate TPA induce[r] ...
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