phorbol myristate acetate
Induction of phosphorylation of human immunodeficiency virus type 1 Nef and enhancement of CD4 downregulation by phorbol myristate acetate.
... The nef gene of the human and simian immunodeficiency viruses (HIV and SIV) encodes a 27 to 34 kDa myristoylated protein that induces downregulation of CD4 from the cell surface and enhances virus infectivity. As shown ...
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Effects of Phorbol Myristate Acetate on the Metabolism and Ultrastructure of Neutrophils in Chronic Granulomatous Disease
... that phorbol myristate acetate (PMA), the active principle of croton oil, stimulates alterations in normal polymorphonuclear leukocytes (PMN) that resemble closely the changes that develop in the ...
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Receptor mediated regulation of superoxide production in human neutrophils stimulated by phorbol myristate acetate
... 'Abbreviations used in this paper: DMSO, dimethylsulfoxide; BS-H, a balanced salt solution; KD, dissociation constant; PMA, phorbol myristate acetate; PMN, neutrophil; Rt, receptor sites[r] ...
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NAD(P)H oxidase activity in human neutrophils stimulated by phorbol myristate acetate
... seen. Phorbol myristate acetate-induced O27 production was noted to be modulated by pH in intact neutrophils, suggesting that NAD(P)H oxidase is localized in the plasma membrane where its activity ...
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Inhibition of vasopressin stimulated water flow in toad bladder by phorbol myristate acetate, dioctanoylglycerol, and RHC 80267 Evidence for modulation of action of vasopressin by protein kinase C
... The action of vasopressin (AVP) in transporting epithelia is mediated by cyclic AMP(cAMP), whereas its effects in hepatocytes are mediated by calcium and phosphoinositides. Based on our recent observation that AVP ...
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Effects of phorbol myristate acetate and sivelestat on the lung injury caused by fat embolism in isolated lungs
... with phorbol myristate acetate (a neutrophil elastase activator) exacerbated, while sivelestat (a neutrophil elastase inhibitor) attenu- ated the FE-induced ...
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Niacinamide mitigated the acute lung injury induced by phorbol myristate acetate in isolated rat's lungs
... effectively abrogated the lung injury caused by phorbol myristate acetate in isolated lungs. Niacinamide dimin- ished the pulmonary hypertension and increase in microvascular permeability following ...
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Phorbol myristate acetate, dioctanoylglycerol, and phosphatidic acid inhibit the hydroosmotic effect of vasopressin on rabbit cortical collecting tubule
... stimulated hydraulic conductivity (Lp) in rabbit cortical collecting tubule (CCT) perfused in vitro at 37 degrees C. In control studies, 10 microU/ml AVP increased Lp (mean +/- SE, X 10(-7) centimeters/atmosphere per ...
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Protein Phosphatase 2A Enhances Activation of Human Immunodeficiency Virus Type 1 by Phorbol Myristate Acetate
... In this report, we demonstrate that, surprisingly, the catalytic subunit of PP2A (PP2Ac) is able to increase the basal activity of the HIV-1 promoter and markedly enhances the promoter’s response to PMA. Additionally, we ...
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Phorbol myristate acetate stimulates ATP dependent calcium transport by the plasma membrane of neutrophils
... calcium concentration [Ca++]i, as assessed by the intracellularly trapped fluorescent calcium indicator Quin 2, but instead slightly lowered [Ca++]i and prevented the rise in [Ca++]i normally induced by the chemotactic ...
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Smoking decreases the response of human lung macrophages to double-stranded RNA by reducing TLR3 expression
... following phorbol myristate acetate/ vitamin D3 differentiation and exposure to cigarette smoke extract, and determined TLR3 protein expression using flow cytometry and ...
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Role of hydrogen peroxide in the neutrophil mediated release of prostacyclin from cultured endothelial cells
... significantly reduced human and bovine endothelial 6-keto-PGF1 alpha release by phorbol myristate acetate-activated neutrophils. Catalase-inhibitable endothelial 6-keto-PGF1 alpha release was also ...
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Non-typeable Haemophilus influenzae decreases cilia beating via protein kinase C epsilon
... including phorbol myristate acetate (PMA) [7], chronic cigarette smoke ex- posure [8], pneumolysin [9], acetaldehyde [10], peroxide [11], and neuropeptide ...
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High-Mobility-Group Protein I Can Modulate Binding of Transcription Factors to the U5 Region of the Human Immunodeficiency Virus Type 1 Proviral Promoter
... upon phorbol myristate acetate stimulation and this induced HMG I appears to both selectively inhibit the binding of basal DNA-binding proteins and enhance the binding of an inducible AP-1 ...
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Vγ9Vδ2 T Cells in Human Legionellosis
... expressing tumor necrosis factor alpha or gamma interferon in response to phorbol myristate acetate were assayed in vitro. At 14 to 16 days after the onset of disease, the expression of both ...
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Cleavage and inactivation of alpha 1 antitrypsin by metalloproteinases released from neutrophils
... with phorbol myristate acetate or fMet-Leu-Phe in the presence or absence of cytochalasin B, released metalloproteinases that catalytically inactivated the plasma serine proteinase inhibitor, alpha ...
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Potential Mediator of Inflammation: PHAGOCYTE DERIVED OXIDANTS SUPPRESS THE ELASTASE INHIBITORY CAPACITY OF ALPHA1 PROTEINASE INHIBITOR IN VITRO
... Human polymorphonuclear leukocytes, monocytes, or pulmonary alveolar macrophages, stimulated in vitro by phorbol myristate acetate PMA, released reactive oxygen species able to suppress [r] ...
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Induction of chronic human immunodeficiency virus infection is blocked in vitro by a methylphosphonate oligodeoxynucleoside targeted to a U3 enhancer element.
... One such oligomer, targeted to an NF-KB enhancer element, inhibited phorbol myristate acetate induction of viral replication and tat-mediated trans activation of the human immunodeficien[r] ...
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Reduced glutathione as a physiological co activator in the activation of peptidylarginine deiminase
... Fig. 4 Release of catalytically active peptidylarginine deiminase ( PAD ) from PMA-stimulated granulocytes. a Microtitre plates coated with human fibrinogen were incubated for 3 h at RT with blood leucocytes resuspended ...
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Soluble CD40L is associated with increased oxidative burst and neutrophil extracellular trap release in Behçet’s disease
... Hydrogen peroxide and superoxide anion quantification Neutrophils and PBMCs were separated by density gra- dient separation using dextran and Ficoll-Hypaque 1077, respectively. Cells were stimulated with (1) 30 ng/ml ...
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