phosphate buffer saline
In vitro quantification of lactate in Phosphate Buffer Saline (PBS) samples
... Abstract— Continuous measurement of lactate levels in the blood is a prerequisite in intensive care patients who are susceptible to sepsis due to their suppressed immune system and increased metabolic demand. Currently, ...
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Electrochemical Behavior of Magnesium Alloys as Biodegradable Materials in Phosphate Buffer Saline Solution
... An extruded magnesium aluminum alloys (AZ31E, AZ91E) donated from Department of mining, Metallurgy and Materials Engineering, Laval University, Canada with chemical composition (wt%): 2.8 Al, 0.96 Zn, 0.28 Mn, 0.0017 Cu, ...
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Statistical analysis of human tuberculosis microarray gene expression data in the bioconductor R package
... The control stimulated samples with phosphate buffer saline (PBS) and experimental unstimulated samples of three different clinical forms of human TB microarray gene expression data [r] ...
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In-vitro antioxidant activity of Cycas beddomei
... The chemical supply of NO was sodium nitroprusside [l0mM] in 0.5 M phosphate buffer, pH 7.4 that spontaneously produces nitric oxide in an aqueous solution. During this investigation, Griess Illosvoy ...
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Osteogenesis evaluation of duck’s feet-derived collagen/hydroxyapatite sponges immersed in dexamethasone
... BMSCs were seeded onto each sponge at a concentra- tion of 1 × 10 5 cells per sponge. Before cell seeding, all sponges were sterilized in 70% ethyl alcohol for 30 min followed by rinsing it 3 times in phosphate ...
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Formulation and In-Vitro Evaluation of Metronidazole Mucoadhesive Microspheres.
... release tablets with a predetermined lag time of 4-5 hrs. Different types of enteric coated tablets were prepared and there drug dissolution profile was studied in 0.1 N HCl (0 to 2hr) and PBS 6.8 (2-24 hr) as ...
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Characterising the role of murine gammaherpesvirus-68 host shutoff protein in mediating cellular mRNA degradation
... Peripheral mononuclear blood cells Processing body Phosphate buffer saline Polymerase chain reaction Plasmacytoid dendritic cells Primary efusion lymphoma Protein kinase R Passive lysis [r] ...
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Anti-inflammatory Potentials of Excretory/Secretory (ES) and Somatic Products of Marshallagia marshalli on Allergic Airway Inflammation in BALB/c Mice
... marshalli in ovalbumin (OVA) induced airway inflamma- tion, mice randomly were divided into four groups each containing five mice: a phosphate buffer saline (PBS) treate[r] ...
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Elucidation of membrane stabilizing Potentials of Methanolic Extract of Vigna unguiculata (cowpea) Linn (seed)
... Hypotonic Solution- Induced Hemolysis The experiments were carried out with hypotonic solution. The test sample consisted of stock eryth- rocyte (RBC) suspension (0.50 mL) with 5 ml of hypotonic solution (50 mM NaCl) in ...
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Phytochemical screening, Antioxidant and Antiangiogenic Properties of Oncoba welwitschii (Oliv.) Gilgn. and Tetrorchidium oppositifolium(Pax. and Khoffm.), Medicinal Plants from Gabon.
... of phosphate buffer saline (PBS) used as a control, the target area has a vascularization percentage of 100%, corresponding to a normal vasculature (Figure 1a, ...
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Development and evaluation of novel Fluticasone Propionate Emulgel for topical drug delivery
... The cumulative % drug release was calculated using standard calibration curve.[22,23] Details of dissolution testing: • Dissolution media: Phosphate buffer saline pH 7.4 • Speed: 50 rpm [r] ...
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Formulation and Evaluation of Diclofenac Potassium Ethosomes
... The comparison of invitro skin permeation of ethosomal, liposomal, hydroethanolic drug solution and phosphate buffer saline PH: 7.4 drug solutions revealed that ethosomal formulation s[r] ...
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Integrity of Metallic Medical Implants in Physiological Solutions
... Materials used in this study were an AISI 316L type stainless steel (SS) with composition in wt.% of: 25.5 Cr, 7.6 Ni, 6.1 Mn, 0.5 Mo, 0.02 C and balance Fe; and Ti-6Al-4V titanium alloy (TA) supplied from Johnson and ...
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Influence of phosphate ions on buffer capacity of soil humic acids
... ions. Buffer properties of soils are strong- ly positively correlated with their sorption ...the buffer capacity of these compounds and HA-rich soils is also high (Lavelle and Spain, ...natural ...
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CRISPR/Cas9-Correctable mutation-related molecular and physiological phenotypes in iPSC-derived Alzheimer’s PSEN2 N141I neurons
... Cells were assayed in a 96-well plate format. Oligomer or vehicle solutions were added to media and allowed to incu- bate for a period of 3 days. Media was then collected and assayed using a lactate dehydrogenase ...
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DEVELOPMENT AND VALIDATION OF NEW ANALYTICAL METHOD FOR OLMESATRON BY RP HPLC
... Initially the mobile phase tried was Methanol: Water and Methanol: Phosphate buffer and ACN: Tri ethyl amine buffer with varying proportions. Finally, the mobile phase was optimized to ACN: TEA ...
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Characterization and antimicrobial efficacy of bovine dermcidin, a novel antimicrobial peptide gene
... Abstract: Description of a novel bovine antimicrobial peptide and its antimicrobial spectrum. RNA isolation and RT-PCR were done from various tissues. DCD peptide was synthesized, and antimicrobial activity was analyzed. ...
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Extraction of sennosides from solutions by chitosan and utilization of the extract for oral dosage form
... Physiological saline, deionized water, 5 mM phosphate buffer (pH 7.0) and ethanol were used as the test solutions. One hundred milligrams of CS-Sen or CS-SFP were added to 10 ml of the test solution ...
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DEVELOPMENT AND EVALUATION OF MUCOADHESIVE BENEDIPINE FORMULATION
... performed and in-expensive method that correlates with the performance of dosage form in human subjects. The conditions of in-vitro dissolution test were well defined, standardized and enable comparison among various ...
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CHEMICAL MODIFICATION OF CHITOSAN AND FORMULATION OF ITS NANOPARTICLES FOR PROTEIN DRUG DELIVERY SYSTEM
... The release profiles in phosphate buffer solution pH 6.8 of Insulin loaded chitosan nanoparticles entrapped in ID2-ID4 batches of uncoated alginate beads are shown in Table 7 and Figure 11. As depicted in ...
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