phosphate-buffered (pH 7.4) saline
Fretting Fatigue Properties of Zr Based Bulk Amorphous Alloy in Phosphate Buffered Saline Solution
... Since such implant devices suffer from cyclic loading during walking, it is important to understand the fatigue behaviors of the amorphous alloys in human body fluid. So far, the authors have reported the following ...
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Development and characterization of magnetic iron oxide nanoparticles with a cisplatin-bearing polymer coating for targeted drug delivery
... dissolved in 500 mL of phosphate-buffered saline (with 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , and 2 mM KH 2 PO 4 adjusted to pH 6.5 with 1 N HCl) and then 28.6 mg HAse was ...
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<p>The Protective Effect of Testosterone on the Ovarian Reserve During Cyclophosphamide Treatment</p>
... with phosphate-buffered saline (PBS), cyclophosphamide (CPA) and cyclophosphamide plus testosterone ...and phosphate-buffered saline ...and phosphate-buffered ...
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COMPARATIVE EVALUATION OF AQUEOUS EXTRACT AND PROTEASE FROM ADHATODA VASICA FOR DETERMINING ITS IMMUNOSUPPRESSIVE ACTIVITY Amit Gupta*, Ratnakar Chitte, Sushama R ChaphalkarDOWNLOAD/VIEW
... In order to compared its immunopharmacological activity of protease (against Casein) and aqueous leaves extract of Adhatoda vasica (using phosphate buffered saline, PBS, pH 7.2) against ...
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Lipolysaccharide Induced Neuroinflammation Is Associated with Alzheimer Like Amyloidogenic Axonal Pathology and Dendritic Degeneration in Rats
... of phosphate-buffered saline, PBS), the pattern of BACE1 immunoreactivity is com- parable between the ipsilateral and contralateral cortex and hippocampal formation, which is generally associated ...
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Investigation on the using of linco-spectin solution for in ovo administration in chicken embryo
... 3: phosphate buffered saline-injected group; whose individuals were injected with phosphate buffered ...Groups 4, 5 and 6 whose individuals were injected with linco-spectin ...
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The Effect of Copper Sulfate on Chicken Embryo During the Incubation Period
... Sterile phosphate buffered saline solution was included as a sham control to rule out a possible negative response caused by the stress of ...
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Formalin inactivation of the lactate dehydrogenase-elevating virus reveals a major neutralizing epitope not recognized during natural infection.
... Plasma from 1-day infected mice about 109 ID5Jml, which is devoid of anti-LDV antibodies 4, was diluted 1:100 in phosphate-buffered saline PBS, and 10-,u samples thereof were mixed with [r] ...
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Fluorosugars inhibit biological properties of different enveloped viruses.
... After three successive washes with prewarmed phosphate-buffered saline, the cells were flooded with 100 ul of pyruvate containing Earle medium buffered with HEPES N-2-hydroxymethyl-piper[r] ...
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Pharmacological evaluation of rat dorsal root ganglion neurons as an in vitro model for diabetic neuropathy
... NaBH 4 (Sigma-Aldrich Chemie, Munich, Germany) dissolved in PHEM buffer was added for 10 ...in phosphate-buffered saline/bovine serum albumin ...in phosphate- buffered ...
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Interaction of Glucose/Sucrose Binding Lectin Isolated from Nigeria Wild Bean with E coli and S aureus
... M phosphate-buffered saline (pH ...Mphosphate-buffered saline (pH ...M phosphate buffered saline was added to make the cell 4% thereafter, it ...
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High-dose dexamethasone induced LPS-stimulated rat alveolar macrophages apoptosis
... ice-cold phosphate- buffered saline (PBS; Thermo Fisher Scientific, Waltham, MA, USA) solution by cannulating the trachea with a short- ened 14-gauge needle attached to a 5-mL syringe, which was ...
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Triage of high-risk human papillomavirus-positive women by methylated POU4F3
... sterile phosphate-buffered saline before biopsy using a cervical brush and were stored at 4 °C until DNA extraction for HPV testing using a HCII hrHPV DNA assay (Digene, Silver Spring, MD, ...
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Therapeutic potency of bee pollen against biochemical autistic features induced through acute and sub acute neurotoxicity of orally administered propionic acid
... The experimental assays for this study were performed on 24 young (approximately 21 days old) male western albino rats (45 to 60 g). The animals were fed on standard pellet diet, water ad libitum and were maintained in a ...
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Study on In Vitro Degradation of Bioabsorbable Polymers Poly (hydroxybutyrate co valerate) (PHBV) and Poly (caprolactone) (PCL)
... The increasing use of bioabsorbable polymeric materials in medicine has stimulated researchers in the materials field to search for solutions for the replacement of metallic artifacts by bioabsorbable polymers. ...
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Attenuation of nicotine evoked Ca2+ influx by antibody to the nicotinic acetylcholine receptor α3 subunits in human embryonic kidney cells
... HEK293-α34 and mock-transfected HEK293 cells were fixed in ice-cold 4% paraformaldehyde (PFA) for 20 min, washed in phosphate-buffered saline (PBS), blocked in PBS containing 1% BSA ...
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Immunoglobulin specific radioimmunoprecipitation assays for quantitation of nasal secretory antibodies to hemagglutinin of type A influenza viruses
... The reaction system contained the following reagents: 100 phosphate-buffered saline, pH 7.2, pl of RIP buffer containing 1 mg of bovine serum albunmin per ml, 0.1 mM ethylenediaminetetra[r] ...
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Analysis of Immunoprecipitated Surface Glycoproteins in Measles Virions and in Membranes of Infected Cells
... Vero cells in roller bottles were washed with Dulbecco phosphate-buffered saline, pH 7.2 PBS, and inoculated with measles virus LEC strain in minimal essential medium without serum at 29[r] ...
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Fecal Components Modulate Human Astrovirus Infectivity in Cells and Reconstituted Intestinal Tissues
... for 4 h at 37°C in the presence of PBS, LPS, PGN, or heat-inactivated ...at 4°C to prevent virus entry, the cells were extensively washed and the viral RNA was extracted and quantified or cell-bound viral ...
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Insufficient ER-stress response causes selective mouse cerebellar granule cell degeneration resembling that seen in congenital disorders of glycosylation
... Cells were fixed with 4% polyformaldehyde at room temperature for 20 min and permeabilized with 0.2% Triton X-100 in PBS for 5 min. Cell death was analyzed using the DeadEnd Fluorometric TUNEL System (Promega) or ...
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