phosphate-buffered saline (0.1 M potassium phosphate, pH 7.2, and 0.14 M NaCl)
Fretting Fatigue Properties of Zr Based Bulk Amorphous Alloy in Phosphate Buffered Saline Solution
... 3 For Co-Cr alloys, there is little difference between the fatigue strength and the fretting fatigue strength in air and PBS-.9–12 There are few papers, however, regarding the fatigue pro[r] ...
6
Covalent Linkage of HIV-1 Trimers to Synthetic Liposomes Elicits Improved B Cell and Antibody Responses
... To determine the in vivo capacities of various liposomes to elicit GC B cells, we immunized mice with phosphate-buffered saline PBS naive, plain liposomes DSPC plus cholesterol, 10 g of[r] ...
15
Chitosan-coated poly(lactic-co-glycolic) acid nanoparticles as an efficient delivery system for Newcastle disease virus DNA vaccine
... with phosphate-buffered saline (PBS), blocked with 5% fat-free milk overnight, and then incubated with an NDV-positive serum at a 1:500 dilution for 1 ...
11
Pharmacological evaluation of rat dorsal root ganglion neurons as an in vitro model for diabetic neuropathy
... in phosphate-buffered saline/bovine serum albumin ...in phosphate- buffered saline/bovine serum albumin; Santa Cruz Biotech- nology, Santa Cruz, CA) as a neuronal stress marker ...
11
Investigation on the using of linco-spectin solution for in ovo administration in chicken embryo
... Nowadays antibiotics are used on a large scale in veterinary and human medicine to cure or prevent diseases. Some antibiotics injected into hatching eggs to eliminate pathogens and prevention of egg transmission of ...
7
Interaction of Glucose/Sucrose Binding Lectin Isolated from Nigeria Wild Bean with E coli and S aureus
... M phosphate-buffered saline (pH ...Mphosphate-buffered saline (pH ...M phosphate buffered saline was added to make the cell 4% thereafter, it was stored in the ...
8
Efficiency of the Injection of Trimethoprim/Sulfamethoxazole Solution on Game Bird Embryonated-Egg During the Late Stage of Development
... Infectious agents cause disease in virtually any susceptible hosts. In the poultry production and medicine, pathogens were eliminated from a line of a breeder by injection of antibiotics into hatching eggs. There is ...
9
Fecal Components Modulate Human Astrovirus Infectivity in Cells and Reconstituted Intestinal Tissues
... HAstV-1 or HAstV-8 was incubated with phosphate-buffered saline (PBS; used as a control for viral inactivation), filtered human stools from healthy volunteers (assessed to be negative for[r] ...
13
Cytokine Responses in Porcine Respiratory Coronavirus-Infected Pigs Treated with Corticosteroids as a Model for Severe Acute Respiratory Syndrome
... The effectiveness and potential immunosuppressive effects of anti-inflammatory glucocorticoids in the lungs of severe acute respiratory syndrome (SARS) patients are undefined. We treated porcine respiratory corona- virus ...
9
Respiratory syncytial virus infection in anti-mu-treated mice.
... in 0.05 ml of phosphate-buffered saline PBS intraperitoneally delivered through a 30-gauge needle and was maintained with 50 ,ug intraperitoneally every other day until a schedule Downlo[r] ...
7
Prolonged Scar-in-a-Jar: an in vitro screening tool for anti-fibrotic therapies using biomarkers of extracellular matrix synthesis
... PBS: Phosphate-buffered saline; PDGF: Platelet-derived growth factor; PI3K: Phosphatidylinositol 3- kinase; PRO-C1: ELISA assessing type I collagen formation; PRO-C3: ELISA assessing type III ...
14
Gastric T lymphocyte responses to Helicobacter pylori in patients with H pylori colonisation
... The four times with bovine serum albuminwashed effect of different amounts of H pyloni on 3H-thymidine phosphate buffered saline followed by two incorporation cpm into lamina propria lym[r] ...
7
Development and characterization of magnetic iron oxide nanoparticles with a cisplatin-bearing polymer coating for targeted drug delivery
... dissolved in 500 mL of phosphate-buffered saline (with 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , and 2 mM KH 2 PO 4 adjusted to pH 6.5 with 1 N HCl) and then 28.6 mg HAse was added. This mixture ...
18
Study on In Vitro Degradation of Bioabsorbable Polymers Poly (hydroxybutyrate co valerate) (PHBV) and Poly (caprolactone) (PCL)
... The increasing use of bioabsorbable polymeric materials in medicine has stimulated researchers in the materials field to search for solutions for the replacement of metallic artifacts by bioabsorbable polymers. ...
9
Folate-modified Annonaceous acetogenins nanosuspensions and their improved antitumor efficacy
... To determine the dissolution kinetics, 4 mL of FA-ACGs- NSps containing 4.0 mg of ACGs were placed in Float-A- Lyzer dialysis cassettes (molecular weight cutoff 12–14 kDa; Spectrum Labs) and immersed in 2 L of 0.1 mol/L ...
15
Annexin A2 binds to vimentin and contributes to porcine reproductive and respiratory syndrome virus multiplication
... PAMs were harvested from 4-week old PRRSV-negative piglets in sterilized phosphate-buffered saline (PBS) by alveolar lavage and then maintained in RPMI-1640 medium. The animal experiments in this ...
12
Expression and secretion of fungal endoglucanase II and chimeric cellobiohydrolase I in the oleaginous yeast Lipomyces starkeyi
... CBP: consolidated bioprocessing; E: amplification efficiency; EG II: endo- glucanase II; gal1 : galactokinase gene; GH: glycoside hydrolase; PBS: phosphate-buffered saline; pyk : pyru[r] ...
16
Transplantation of cryopreserved human umbilical cord blood-derived endothelial progenitor cells induces recovery of carotid artery injury in nude rats
... with phosphate-buffered saline (PBS, Sigma) and suspended at 5 × 10 7 cells/mL with a final concentration of 10% dimethyl sulfoxide (DMSO) (Sigma) and 20% fetal bovine serum (FBS) ...
12
Evaluation of cellular antioxidant and antiproliferative activities of five main phyllanthus emblica L. cultivars in China
... in phosphate buffered saline wash protocol whereas, Boligan (3735±217 µmol of quercetin equivalents/100 g) had the highest cellular antioxidant activity and Tianyougan (2025±171 µmol of quercetin ...
9
Novel metastatic models of esophageal adenocarcinoma derived from FLO-1 cells highlight the importance of E-cadherin in cancer metastasis
... Tumor xenografts To assess tumorigenicity and metastatic potential of esophageal cancer cell lines, 5 million cells suspended in 100 µl of 1:1 phosphate buffered saline PBS and matrigel [r] ...
17