phosphate buffered saline (pH 7.5)
Fretting Fatigue Properties of Zr Based Bulk Amorphous Alloy in Phosphate Buffered Saline Solution
... At high cycles, the fretting fatigue strength of the amorphous alloy in PBS(-) is approximately twice that in air. The fretting fatigue life consists of crack initiation life and crack propagation life. At high-stress ...
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Development and characterization of magnetic iron oxide nanoparticles with a cisplatin-bearing polymer coating for targeted drug delivery
... of phosphate-buffered saline (with 137 mM NaCl, ...for 5 minutes at room temperature to carry out the degradation, followed by vigorous stirring at 95 ° C for 15 minutes in an oil ...
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<p>The Protective Effect of Testosterone on the Ovarian Reserve During Cyclophosphamide Treatment</p>
... 1× phosphate-buffered saline, then incubated with Histo fi ne Simple Stain MAX PO (multi; Nichirei) for 30 min at room ...1×phosphate-buffered saline and visualized by incubat- ...
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Lipolysaccharide Induced Neuroinflammation Is Associated with Alzheimer Like Amyloidogenic Axonal Pathology and Dendritic Degeneration in Rats
... of phosphate-buffered saline, PBS), the pattern of BACE1 immunoreactivity is com- parable between the ipsilateral and contralateral cortex and hippocampal formation, which is generally associated ...
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Therapeutic potency of bee pollen against biochemical autistic features induced through acute and sub acute neurotoxicity of orally administered propionic acid
... The experimental assays for this study were performed on 24 young (approximately 21 days old) male western albino rats (45 to 60 g). The animals were fed on standard pellet diet, water ad libitum and were maintained in a ...
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Original Article Effects and mechanisms of oridonin in the treatment of acute respiratory distress syndrome mice
... sterile phosphate buffered saline (PBS) was instilled into the trachea; an experimental group, in which after the establishment of ARDS mice models, different doses of ORI (30 mg/kg, 90 mg/kg, 150 ...
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The Effect of Copper Sulfate on Chicken Embryo During the Incubation Period
... Fertile partridge eggs (Chukar partridge) from the partridge breeders which are maintained in the standard condition and the adequate nutritional plan, with the average egg-weight of 20 ± 0.8, were collected from a local ...
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Fluorosugars inhibit biological properties of different enveloped viruses.
... After three successive washes with prewarmed phosphate-buffered saline, the cells were flooded with 100 ul of pyruvate containing Earle medium buffered with HEPES N-2-hydroxymethyl-piper[r] ...
5
Pharmacological evaluation of rat dorsal root ganglion neurons as an in vitro model for diabetic neuropathy
... in phosphate-buffered saline/bovine serum albumin ...containing 5% normal goat serum ...in phosphate- buffered saline/bovine serum albumin; Santa Cruz Biotech- nology, ...
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Interaction of Glucose/Sucrose Binding Lectin Isolated from Nigeria Wild Bean with E coli and S aureus
... for 5 minutes at room ...for 5 minutes at temperature with 0.01 M phosphate-buffered saline (pH ...Mphosphate-buffered saline (pH ...M phosphate ...
8
High-dose dexamethasone induced LPS-stimulated rat alveolar macrophages apoptosis
... with 5 mL ice-cold phosphate- buffered saline (PBS; Thermo Fisher Scientific, Waltham, MA, USA) solution by cannulating the trachea with a short- ened 14-gauge needle attached to a 5-mL ...
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COMPARATIVE EVALUATION OF AQUEOUS EXTRACT AND PROTEASE FROM ADHATODA VASICA FOR DETERMINING ITS IMMUNOSUPPRESSIVE ACTIVITY Amit Gupta*, Ratnakar Chitte, Sushama R ChaphalkarDOWNLOAD/VIEW
... In order to compared its immunopharmacological activity of protease (against Casein) and aqueous leaves extract of Adhatoda vasica (using phosphate buffered saline, PBS, pH 7.2) against ...
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Study on In Vitro Degradation of Bioabsorbable Polymers Poly (hydroxybutyrate co valerate) (PHBV) and Poly (caprolactone) (PCL)
... The increasing use of bioabsorbable polymeric materials in medicine has stimulated researchers in the materials field to search for solutions for the replacement of metallic artifacts by bioabsorbable polymers. ...
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Immunoglobulin specific radioimmunoprecipitation assays for quantitation of nasal secretory antibodies to hemagglutinin of type A influenza viruses
... The reaction system contained the following reagents: 100 phosphate-buffered saline, pH 7.2, pl of RIP buffer containing 1 mg of bovine serum albunmin per ml, 0.1 mM ethylenediaminetetra[r] ...
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Investigation on the using of linco-spectin solution for in ovo administration in chicken embryo
... 3: phosphate buffered saline-injected group; whose individuals were injected with phosphate buffered ...4, 5 and 6 whose individuals were injected with linco-spectin solution at ...
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Cigarette smoke exposure facilitates allergic sensitization in mice
... of 5 Kentucky Reference cigarettes (2R4F without filter)(Uni- versity of Kentucky, Lexington, KY, USA) for 7 min, 4 times a ...= 7). During air or smoke exposure, con- current challenges with ...
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Analysis of Immunoprecipitated Surface Glycoproteins in Measles Virions and in Membranes of Infected Cells
... Vero cells in roller bottles were washed with Dulbecco phosphate-buffered saline, pH 7.2 PBS, and inoculated with measles virus LEC strain in minimal essential medium without serum at 29[r] ...
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Novel metastatic models of esophageal adenocarcinoma derived from FLO-1 cells highlight the importance of E-cadherin in cancer metastasis
... site remained tumor-free 6 months post injection. Once subcutaneous tumors reached endpoint volume, necropsy was performed on all animals to search for evidence of macro-metastasis. We found that all 8 cell lines were ...
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CRISPR/Cas9-Correctable mutation-related molecular and physiological phenotypes in iPSC-derived Alzheimer’s PSEN2 N141I neurons
... Previous reports have shown that iPSC lines carrying FAD mutations may display an enhanced susceptibility to noxious stimuli, such as high concentrations of Aβ42 oligomers [2]. We therefore tested whether our BFCNs from ...
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Insufficient ER-stress response causes selective mouse cerebellar granule cell degeneration resembling that seen in congenital disorders of glycosylation
... failure to thrive [1,2]. There are about 70 reported gene defects that affect N-linked and/or O-linked glycosyla- tion pathways, resulting in truncated or completely missing glycans and leading to the pathogenesis of ...
8