Phosphorylation and kinases
Phosphorylation of Notch1 by Pim kinases promotes oncogenic signaling in breast and prostate cancer cells
... Pim kinases by Notch proteins, we analysed the effects of Notch1 on Pim protein levels in MCF-7 and PC-3 ...Pim kinases are constitutively active whenever expressed [33], changes in their expression levels ...
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Conserved Protein Kinases Encoded by Herpesviruses and Cellular Protein Kinase cdc2 Target the Same Phosphorylation Site in Eukaryotic Elongation Factor 1δ
... protein kinases encoded by herpesviruses, including UL13 of herpes simplex virus type 1 (HSV-1), UL97 of human cytomegalovirus, and BGLF4 of Epstein-Barr virus ...of phosphorylation by the recombinant UL13 ...
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Tyrosine Phosphorylation of Tau by the Src Family Kinases Lck and Fyn
... of phosphorylation at several different sites by mutation of one of them: ...Src-family kinases have SH3 domains which can bind to tau [40-42], and Src-SH3 and Fyn-SH3 show some distinct differences in ...
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Analysis of changes in phosphorylation of Receptor Tyrosine Kinases: antibody arrays
... Tyrosine kinases are mainly classifi ed into two groups, as receptor tyrosine kinase (RTK) and non-receptor tyrosine kinase ...analyse phosphorylation and dephosphorylation of ...
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Detection of the Potyviral Genome-Linked Protein VPg in Virions and Its Phosphorylation by Host Kinases
... the phosphorylation patterns correlate with the phenotypic differences in ...that phosphorylation of VPg could have functional significance for the virus-host ...the phosphorylation pat- terns ...
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Inhibition of protein phosphorylation modulates expression of the Jak family protein tyrosine kinases.
... The involvement of Jak proteins in response to other bio- logical response modifiers such as growth hormone, leukemia inhibitory factor, ciliary neurotrophic factor, interleukin-3, and erythropoietin has been ...
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TGF-β1 increases proliferation of airway smooth muscle cells by phosphorylation of map kinases
... The proliferation of several phenotypes of cells is medi- ated by growth factor or cytokine induced mitogen-acti- vated protein kinases (MAPKs), a family of serine- threonine protein. MAPKs consist of ...
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Two new families of the FtsZ-tubulin protein superfamily implicated in membrane remodeling in diverse bacteria and archaea
... Analysis of the gene context of cell division related genes in archaea, including serine/threonine protein kinases (PK) implicated in FtsZ phosphorylation, revealed a locus in Halorhabd[r] ...
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Modulation of the trans-suppression activity of hepatitis C virus core protein by phosphorylation.
... vitro phosphorylation experiment (see Materials and Methods for a detailed explanation of plasmid construction) ...These phosphorylation signals are PKA and PKC dependent because in the absence of these two ...
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Chronic lymphocytic leukemia B cells contain anomalous Lyn tyrosine kinase, a putative contribution to defective apoptosis
... tyrosine phosphorylation, we cultured the leukemic cells obtained from CLL patients in the presence of the following inhibitors selective for different tyrosine kinases: PP2 and SU6656 (Src family) (21); ...
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MAP Kinase Regulation of the Candida albicans Pheromone Pathway
... enhanced phosphorylation in the WT strain; this appears to result from Cek1 because it is missing in all the cek1 deletion strains and enhanced in the cek2 deletion ...the phosphorylation status of GFP ...
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The role of the phosphatidylinositol transfer protein and the small GTP-ASE ARF in regulated exocytosis from RBL-2H3 mast cells
... the phosphorylation of a number of receptor-associated proteins, PLCy1 and y2 (49,50), the 85kDa subunit of phosphatidylinositol 3-kinase (51), Vav, a guanine nucleotide exchange factor for rac (52), the adaptor ...
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LRRK2 phosphorylates pre-synaptic N-ethylmaleimide sensitive fusion (NSF) protein enhancing its ATPase activity and SNARE complex disassembling rate
... threonine kinases: NSF is phosphorylated by Pctaire1 at S569 in the D2 domain, and this phosphorylation re- duces NSF oligomerization [25]; NSF is also phosphory- lated by PKC in vitro at Ser-237 of the ...
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Controlled integration of the Ty5 retrotransposon in Saccharomyces ceverisiae [i e cerevisiae]
... Prompted by the discovery of targeting domain phosphorylation, a total of 109 kinase deletion strains were screened for kinases that affect Ty5 target specificity.. Among eight candidate[r] ...
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Amino acid identity at one position within the alpha1 helix of both the histidine kinase and the response regulator of the WalRK and PhoPR two component systems plays a crucial role in the specificity of phosphotransfer
... sensor kinases or by high-energy intermediates, such as acetyl phosphate, even in the absence of the cognate PhoR ...PhoP phosphorylation; (ii) activation of PhoP was further increased by making 4 (strain ...
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PP4 and PP2A regulate Hedgehog signaling by controlling Smo and Ci phosphorylation
... In response to Hh stimulation, many Hh pathway components are phosphorylated and a number of kinases have been identified. However, the phosphatase(s) involved have remained elusive. To explore whether ...
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Cytoskeletal signaling proteins : complex regulatory mechanisms and roles in innate immunity
... Western blotting (Fig. 4.3A). Pyk2 was detected in Syk immune complexes and Syk was found in Pyk2 immune complexes. Interestingly, the Pyk2 auto-phosphorylation site is not required for this interaction, which ...
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Mechanism and Regulation of Nucleocytoplasmic Trafficking of Smad
... receptor kinases and intracellular Smad proteins as the critical components of the signaling mechanism ...direct phosphorylation by the TGF-b receptor kinases ...
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Multistep activation of the Helicobacter pylori effector CagA
... tyrosine phosphorylation by host cell ...CagA phosphorylation, which indicates that c-Src and c-Abl kinases sequentially phosphorylate ...two phosphorylation events need not occur on the same ...
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Targeting of glioblastoma cell lines and glioma stem cells by combined PIM kinase and PI3K-p110 inhibition
... serine/threonine kinases with important roles in protein synthesis and cancer cell ...PIM kinases or pharmacological inhibition of PIM kinases by SGI-1776 or AZD-1208 results in reduced ...
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