phytohemagglutinin-P
Evaluation of biological safety in vitro and immunogenicity in vivo of recombinant Escherichia coli Shiga toxoids as candidate vaccines in cattle
... Neither incubation of PHA-P (phytohemagglutinin-P) stimulated PBMC with rStx WT nor incubation with rStx mut led to a significant in- crease in the percentage of late apoptotic/necrotic [r] ...
14
Depression of the lymphocyte transformation response to microbial antigens and to phytohemagglutinin during pregnancy
... Lymphocyte transformation (LT) responses to Chlamydia trachomatis, to four other microbial antigens, and to phytohemagglutinin (PHA) were studied in 201 women during pregnancy and/or 3-18 wk postpartum. The LT ...
11
Human cardiac stem cells inhibit lymphocyte proliferation through paracrine mechanisms that correlate with indoleamine 2,3-dioxygenase induction and activity
... Carboxyfluorescein succinimidyl ester (CFSE)-labeled and phytohemagglutinin (PHA)-activated human peripheral blood mononuclear cells (hPBMCs) were cultured with mitomycin-treated human c[r] ...
7
Decreased ex vivo production of interferon gamma is associated with severity and poor prognosis in patients with lupus
... Background: Lupus pathogenesis is closely associated with interferon gamma (IFN- γ ), which plays a central role in innate and adaptive immunity. The aim of this study was to evaluate the ex vivo production of IFN- γ ...
9
The effect of epsilon amino caproic acid and other inhibitors of proteolysis upon the response of human peripheral blood lymphocytes to phytohemagglutinin
... Previous work has suggested that intracellular proteolysis may play a role in lymphocyte stimulation. An inhibitor of proteolysis, epsilon amino caproic acid (EACA) was studied for its effect on the lymphocyte response ...
13
Impaired Lymphocyte Transformation in Hodgkin's Disease EVIDENCE FOR DEPLETION OF CIRCULATING T LYMPHOCYTES
... The kinetics of lymphocyte transformation induced by phytohemagglutinin PHA and pokeweed mitogen PWM were studied daily, with blood lymphocytes from normal individuals and from untreated[r] ...
11
Phytohemagglutinin Response in Systemic Lupus Erythematosus: RECONSTITUTION EXPERIMENTS USING HIGHLY PURIFIED LYMPHOCYTE SUBPOPULATIONS AND MONOCYTES
... The phytohemagglutinin PHA response of lymphocytes from untreated patients with systemic lupus erythematosus SLE was studied using highly purified subpopulations of cells involved in the[r] ...
14
Human lymphocyte metabolism Effects of cyclic and noncyclic nucleotides on stimulation by phytohemagglutinin
... The effects of extracellular nucleotides and agents which elevate intracellular cyclic adenosine 3¢,5¢-monophosphate (cyclic AMP) concentrations on human lymphocyte metabolism have been studied. Aminophylline, ...
8
The rapid induction by phytohemagglutinin of increased α aminoisobutyric acid uptake by lymphocytes
... The effect of phytohemagglutinin (PHA) on the ability of human lymphocytes to transport the nonutilizable amino acid, a-aminoisobutyric acid (AIB) has been studied. PHA binds rapidly to plasma membrane receptor ...
10
Regulation of Sodium and Potassium Transport in Phytohemagglutinin Stimulated Human Blood Lymphocytes
... Phytohemagglutinin (PHA) or concanavalin A treatment of lymphocytes causes an increase in membrane permeability so that the leak rates of Na and K increase 1.5- to 2-fold. Active Na and K transport increase ...
9
Cyclic adenosine 3′,5′ monophosphate in human lymphocytes Alterations after phytohemagglutinin stimulation
... We have studied cyclic adenosine 3¢,5¢-monophosphate (cyclic AMP) concentrations in human peripheral blood lymphocytes after stimulation with phytohemagglutinin (PHA), isoproterenol, prostaglandins, and ...
11
Homocystinuria: Heterozygote Detection using Phytohemagglutinin Stimulated Lymphocytes
... Using phytohemagglutinin (PHA)-stimulated lymphocytes as a readily available source of this enzyme, its activity has been compared in 48 control subjects, seven homozygotes affected with homocystinuria, and 17 ...
5
The interaction of human macrophages and lymphocytes in the phytohemagglutinin stimulated production of interferon
... response, phytohemagglutinin was required for the duration of the culture, and both cell types in association were ...from phytohemagglutinin-stimulated macrophages or lymphocytes could not substitute for ...
11
Cystathionine Synthase Activity in Human Lymphocytes: Induction by Phytohemagglutinin
... Uncultured human leukocytes contain no detectable cystathionine synthase activity. A method is described in which the addition of phytohemagglutinin (PHA) to short-term lymphocyte cultures results in a significant ...
5
TREATMENT OF MUCOCUTANEOUS CANDIDIASIS WITH TRANSFER FACTOR
... with CMCD associated with negative can- dida skin tests, impaired response of lymphocytes to candida stimulation in vitro, normal response of lymphocytes to phytohemagglutinin stimulatio[r] ...
10
Adenosine metabolism in phytohemagglutinin stimulated human lymphocytes
... The association of a human genetic deficiency of adenosine deaminase activity with combined immunodeficiency prompted a study of the effects of adenosine and of inhibition of adenosine deaminase activity on human ...
14
Lymphokine stimulation of collagen accumulation
... mitogen phytohemagglutinin, have been shown to cause enhanced collagen accumulation by human embryonic lung fibroblasts (WI-38), as measured by hydroxyproline content of fibroblast monolayers, [14C] proline ...
14
Replication of Poliovirus in Phytohemagglutinin-stimulated Human Lymphocytes
... 40 [ W0 DISCUSSION In our study of poliovirus replication in leukocytes, the original observations of Gresser and Chany could be confirmed 10, namely, that nonstimulated leukocyte cultur[r] ...
7
Potasssium transport in human blood lymphocytes treated with phytohemagglutinin
... GLOSSARY OF SYMBOLS Time constant of K+ diffusion, min-' Time constant of K+ diffusion as measured in a 42K+ efflux experiment, min-' Time constant of K+ diffusion as measured by the rat[r] ...
13
Partial Purification of Osteoclast Activating Factor from Phytohemagglutinin Stimulated Human Leukocytes
... When the medium from activated leukocytes cultured with autologous plasma was fractionated by gel filtration on Sephadex, bone resorbing activity eluted both with plasma proteins and in [r] ...
9