Phytohemagglutinin, PHA
Cystathionine Synthase Activity in Human Lymphocytes: Induction by Phytohemagglutinin
... of phytohemagglutinin (PHA) to short-term lymphocyte cultures results in a significant induction of enzymatic ...This PHA- stimulated activity has characteristics that resemble those previously ...
5
Decreased ex vivo production of interferon gamma is associated with severity and poor prognosis in patients with lupus
... Background: Lupus pathogenesis is closely associated with interferon gamma (IFN- γ ), which plays a central role in innate and adaptive immunity. The aim of this study was to evaluate the ex vivo production of IFN- γ ...
9
MHC restricted fratricide of human lymphocytes expressing survivin specific transgenic T cell receptors
... ulation with either phytohemagglutinin PHA or a combination of CD3- and CD28-specific antibodies, activated HLA-A2– lymphocytes were not recognized by effector cells expressing Tg-TCR, e[r] ...
10
Homocystinuria: Heterozygote Detection using Phytohemagglutinin Stimulated Lymphocytes
... Using phytohemagglutinin (PHA)-stimulated lymphocytes as a readily available source of this enzyme, its activity has been compared in 48 control subjects, seven homozygotes affected with homocystinuria, and ...
5
Identification of a New Quaternary Neutralizing Epitope on Human Immunodeficiency Virus Type 1 Virus Particles
... MAb 2909 was also tested for neutralization against the parental form of virus SF162 in the GHOST cell neutralization assay 5 and the phytohemagglutinin PHA-stimulated PBMC assay 14; 50%[r] ...
6
Glucagon-like peptide-1 receptor (GLP-1R) signaling ameliorates dysfunctional immunity in COPD patients
... Reduced IFN-γ production stimulated by phytohemagglutinin (PHA) and increased programmed cell death protein 1 (PD-1) expression on T cells were observed in COPD patients compared with [r] ...
12
Cytokine Responses to Stimulation of Whole Blood from Patients with Buruli Ulcer Disease in Ghana
... with phytohemagglutinin (PHA) and tuberculin purified protein derivative (PPD) of whole blood from 39 (23 early- and 16 late-stage) BUD patients and 39 healthy control subjects in ...
5
Non-immunogenic dextran-coated superparamagnetic iron oxide nanoparticles: a biocompatible, size-tunable contrast agent for magnetic resonance imaging
... with Pha in the presence or absence of ...control; Pha, phytohemagglutinin; sD, standard deviation; seM, standard error of the mean; sPIONdex, dextran-coated superparamagnetic iron oxide ...
16
Induction of the Platelet Release Reaction by Phytohemagglutinin
... erythroagglutinating phytohemagglutinin (E-PHA) from Phaseolus vulgaris binds to the surface of intact human platelets and that adenylate cyclase activity in the particulate fraction of E-PHA-treated ...
9
Potasssium transport in human blood lymphocytes treated with phytohemagglutinin
... lymphocytes. PHA, however, did not produce an increase in lymphocyte K+ ...after PHA treatment represented concentrative ...from PHA treated lymphocytes ...
13
SLC39A8 gene encoding a metal ion transporter: discovery and bench to bedside
... Osteoarthritis; PHA: Phytohemagglutinin; PLCB1: Phospholipase-C-beta-1 gene; RI: Recombinant inbred; rvZIP8: Stably transfected MEFs containing ZIP8 cDNA via retroviral infection; Sglt2 > Cre > ...
21
Mesenchymal stem cells from umbilical cord matrix, adipose tissue and bone marrow exhibit different capability to suppress peripheral blood B, natural killer and T cells
... E2; PHA: Phytohemagglutinin; PKC: Protein kinase C; SSC: Side scatter; STAT3: Signal transducer and activator of transcription 3; TCR: T cell receptor; TGF- β : Transforming growth factor- β ; TLR: Toll ...
16
Phytohemagglutinin Response in Systemic Lupus Erythematosus: RECONSTITUTION EXPERIMENTS USING HIGHLY PURIFIED LYMPHOCYTE SUBPOPULATIONS AND MONOCYTES
... monocytes. Cell transformation was quantitated using both tritiated thymidine ([ 3 H]-TdR) incorporation into DNA and cytofluorographic determination of cellular DNA content. Dose- response curves using six ...
14
Evaluation of biological safety in vitro and immunogenicity in vivo of recombinant Escherichia coli Shiga toxoids as candidate vaccines in cattle
... Neither incubation of PHA-P (phytohemagglutinin-P) stimulated PBMC with rStx WT nor incubation with rStx mut led to a significant in- crease in the percentage of late apoptotic/necrotic [r] ...
14
Revelation of the ability of Burkholderi a sp. USM (JCM 15050) PHA synthase to polymerize 4-hydroxybutyrate monomer
... the PHA biosynthetic genes from a locally isolated Burkholderia ...The PHA biosynthetic genes of Burkholderia sp. consisted of a PHA synthase (phaC), β-ketothiolase (phaA), acetoacetyl-CoA reduc- ...
9
Production of Medium Chain Length Poly (3 Hydroxyalkanoates) from Saponified Palm Kernel Oil by Pseudomonas putida: Kinetics of Batch and Fed Batch Fermentations
... both the cell concentration and its cellular content; therefore, a minimal supply of these are necessary to achieve a high PHA productivity in a jar fermenter operation. This was based on their results from the ...
9
ISOLATION, PRODUCTION AND EXTRACTION OF BACTERIAL POLYHYDROXYALKANOATE ENZYME
... of PHA are rubbery and flexibility in nature and have low ...1992). PHA polymer of medium length chain is more potential for application due to increase elasticity and decrease crystallinity as compared to ...
10
Discovery of a mcl-PHA with unexpected biotechnical properties: the marine environment of French Polynesia as a source for PHA-producing bacteria
... addition, PHA comprise a large class of ...long-chain-length PHA. The short-chain-length PHA (scl-PHA) are ...medium-chain-length PHA (mcl-PHA) are syn- thetized by only a few ...
9
Cellular Immune Responses to Extracellular Streptococcal Products in Rheumatic Heart Disease
... The mean phytohemagglutinin dose responses of tonsillar and peripheral lymphocytes from RHD patients were essentially indistinguishable from those of controls. In contrast, the responses of tonsillar and ...
8
The in vitro response of bovine peripheral blood lymphocytes to phytohemagglutinin and tuberculin
... On the basis of several lines of evidence, it is likely that the type of ^ vitro response of bovine lymphocytes to tuberculin FPD is distinct from the response to PK^, and represents ant[r] ...
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