Plasmodium species
Detection and identification of human Plasmodium species with real time quantitative nucleic acid sequence based amplification
... of Plasmodium parasites in Giemsa-stained slides with standard microscopy according to WHO recommen- dations [11] or PCR methods as established in the con- tributing medical ...four Plasmodium ...
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Seroepidemiology of Plasmodium species infections in Zimbabwean population
... the species of Plasmodium causing ...in species differentiation owing to subtle differences in the morphology of blood stage parasites ...the species of Plasmo- dium causing disease being ...
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Plasmodium species differentiation by non expert on line volunteers for remote malaria field diagnosis
... some Plasmodium spe- cies [9], the examination of Giemsa-stained thick and thin films of blood by light microscopy from malaria patients, remains the gold standard for malaria diagno- sis in most parts of the ...
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Limitations of microscopy to differentiate Plasmodium species in a region co endemic for Plasmodium falciparum, Plasmodium vivax and Plasmodium knowlesi
... by Plasmodium falciparum , the relative contribu- tion of the non-falciparum Plasmodium species to the global malaria burden is increasing as incidence of P ...these species is ...
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Molecular Identification of Plasmodium Species in Malaria in Zimbabwe by 18S Ribosequencing
... genus Plasmodium and are transmitted to humans by the Anopheles species of mosquitoes ...known species of Plasmodium that cause human malaria and these are Plasmodium falciparum, ...
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Giemsa stained thick blood films as a source of DNA for Plasmodium species specific real time PCR
... four Plasmodium species with varying parasite densities or only gametocytes, mixed infections, and negative samples and was stored for up to 12 ...four Plasmodium species with four ...
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Simple Real Time PCR and Amplicon Sequencing Method for Identification of Plasmodium Species in Human Whole Blood
... study, Plasmodium infections accounted for 21% of 6,957 patients with fever in a cohort of 24,920 ill returned travelers ...infecting species is essential for effective treat- ment of ...These ...
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Adaptive Evolution of RH5 in Ape Plasmodium species of the Laverania Subgenus
... We have obtained a much more comprehensive data set, including Rh5 gene sequences from all six known species of ape Laverania parasites related to P. falciparum. We find that the two residues in RH5 identified by ...
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Performance of three multi species rapid diagnostic tests for diagnosis of Plasmodium falciparum and Plasmodium vivax malaria in Oromia Regional State, Ethiopia
... major Plasmodium species causing malaria in Ethiopia are Plasmodium falciparum and Plasmodium vivax, for which the national guidelines prescribe treat- ment with the artemisinin combination ...
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The epidemiology of subclinical malaria infections in South East Asia: findings from cross sectional surveys in Thailand–Myanmar border areas, Cambodia, and Vietnam
... of PCR grade water resulting in a concentration factor defined by the original blood volumes (100–2000 μ L) divided by the resuspension volume (10–50 µL). Two microlitres of resuspended DNA was used as template in the ...
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Differential induction of malaria liver pathology in mice infected with Plasmodium chabaudi AS or Plasmodium berghei NK65
... Differently from P. chabaudi AS-infected mice, livers from P. berghei NK65-infected mice also showed a dark- brown coloration in agreement with the significantly higher hepatic Hz deposition (Fig. 2a). In parallel with ...
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Identification and characterization of areas of high and low risk for asymptomatic malaria infections at sub village level in Ratanakiri, Cambodia
... The association of individuals’ characteristics or poten- tial risk behavior with the malaria prevalence was ana- lysed as previously described [28] by fitting generalized linear mixed models (GLMM) with a binomial error ...
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Applied Genomics: Data Mining Reveals Species Specific Malaria Diagnostic Targets More Sensitive than 18S rRNA
... implementing species-appropriate treat- ment and saving ...detecting Plasmodium, differentiating between Plasmodium species, and detecting subclinical ...for Plasmodium falciparum and ...
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Repetitive sequences in malaria parasite proteins
... al., 2005) and ankyrin (Weng et al., 2014) and deletion of the gene causes a loss of knob structures, decreases adhesion under flow conditions (Crabb et al., 1997), and reduces erythrocyte rigidity (Glenister et al., ...
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Avian malaria on Madagascar: bird hosts and putative vector mosquitoes of different Plasmodium lineages
... isolated Plasmodium lineages were compared to previously published sequences in GenBank and thus ...lineage Plasmodium minuoviride haplotype CCA0640 ...Because species of the genus Uranotaenia are ...
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Non invasive surveillance for Plasmodium in reservoir macaque species
... targeted. Plasmodium species have a small mitochondrial genome (mtDNA), spanning only 6 kb but with copy numbers ranging from 20 to 100 per parasite [35, 36], whereas other common molecular targets ...
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A comparison of two PCR protocols for the differentiation of Plasmodium ovale species and implications for clinical management in travellers returning to Germany: a 10 year cross sectional study
... Human Plasmodium ovale spp. malaria is caused by two different sympatric Plasmodium species, namely P. ovale curtisi, which was considered the “classic” human patho- gen, and P. ovale wallikeri, ...
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Misclassification of Plasmodium infections by conventional microscopy and the impact of remedial training on the proficiency of laboratory technicians in species identification
... preserving species specific diag- nostic details such as red blood cell shape and size, para- site morphology, pigment color and presence of cell inclusions which would otherwise be lost during thick film ...in ...
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Plasmodium berghei bio burden correlates with parasite lactate dehydrogenase: application to murine Plasmodium diagnostics
... human Plasmodium species [33, ...all species [43, ...rodent species and strains of Plasmodium was reported by Talman et ...different species of murine Plasmodium: 86 % ...
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Malaria in Mauritania: retrospective and prospective overview
... and Plasmodium species distribution are critical for guiding national efforts to improve laboratory diagnosis, imple- ment rapid and effective treatment, and coordinate and plan malaria elimination from the ...
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