Platelet lysate
Pathogen free, plasma poor platelet lysate and expansion of human mesenchymal stem cells
... origin. Platelet lysate (PL) contains a wide series of growth factors, thanks to which platelets (PLT) are capable to mediate tissue repair at in- jured sites in physio-pathological conditions; for these ...
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Expanding CAR T cells in human platelet lysate renders T cells with in vivo longevity
... 1928z: Second generation CAR targeting CD19 with CD28 costimulatory domain; ABS: Human AB serum; B-ALL: B lymphoblastic leukemia/lymphoma; CAR: Chimeric antigen receptor; Cas9: CRISPR associated protein 9; CRISPR: ...
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Differentiation of Rat Bone Marrow Mesenchymal Stem Cells into Adipocytes and Cardiomyocytes after Treatment with Platelet Lysate
... Platelet-rich plasma (PRP) was used for preparing platelet lysate. 16,18 Following deep anesthesia and cervical dislocation as described above, blood samples of 10 male rats were collected via ...
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The use of lumbar epidural injection of platelet lysate for treatment of radicular pain
... Platelet lysate (PL) is created by lysing platelets and re- moving the cell debris, resulting in a GF-rich injectate, devoid of other platelet material (Doucet et ...
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Mechanical fibrinogen depletion supports heparin free mesenchymal stem cell propagation in human platelet lysate
... human platelet lysate (pHPL) is an efficient alternative to xenogenic supplements for ex vivo expansion of mesenchymal stem cells (MSCs) in clinical ...
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Use of platelet lysate for bone regeneration - are we ready for clinical translation?
... human platelet lysate (PL) offers an effective ...material, platelet concentration, leukocyte depletion, and the method of platelet ...
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Autologous platelet lysate local injections for the treatment of refractory lateral epicondylitis
... Background: The purpose of this study was to investigate the efficacy and safety of autologous platelet lysate (APL) local injections in reducing pain and improving function in patients with refractory ...
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Platelet lysate enhances synovial fluid multipotential stromal cells functions: Implications for therapeutic use
... Yu, Human platelet lysate supports ex vivo expansion and enhances osteogenic differentiation of human bone marrow-derived mesenchymal stem cells, Cell biology international 356 2011 639-[r] ...
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The neuroprotective activity of heat-treated human platelet lysate biomaterials manufactured from outdated pathogen-reduced (amotosalen/UVA) platelet concentrates
... A mixture of cortical and hippocampal neurons cultures was performed as described previously [25, 26]. Briefly, primary cultures were prepared from 18.5 days’ mouse embryos (C57BL/6 J) by collecting the forebrains in ...
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Platelet lysate as a substitute for animal serum for the ex-vivo expansion of mesenchymal stem/stromal cells: present and future
... human platelet-poor plasma; IDO, indoleamine 2,3-dioxygenase; IGF-1, insulin-like growth factor-1; IgG, Human immunoglobulin G; MSC, mesenchymal stem/stromal cell; PB, peripheral blood; PDGF, ...
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Platelet lysate induces chondrogenic differentiation of umbilical cord-derived mesenchymal stem cells
... Purpose: Articular cartilage has a poor capacity for self-repair, and thus still presents a major challenge in orthopedics. Mesenchymal stem cells (MSCs) are multipotent stem cells with the potential to differentiate ...
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Potency testing of mesenchymal stromal cell growth expanded in human platelet lysate from different human tissues
... Background: Mesenchymal stromal cells (MSCs) have been largely investigated, in the past decade, as potential therapeutic strategies for various acute and chronic pathological conditions. MSCs isolated from different ...
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Human platelet lysate in mesenchymal stromal cell expansion according to a GMP grade protocol: a cell factory experience
... Experiments where performed at passage 3 or 4 because these correspond to the maximum number of expansions allowed by our clinical protocol and Investigational Medi- cinal Product Dossier (IMPD). Our IMPD is based on ...
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Application of platelet-rich plasma and platelet lysate in the treatment of experimental lymphocutaneous sporotrichosi
... plasma lysate preparation Expired platelet product bags were used to prepare a PRP sample and platelet ...of platelet was centrifuged at 1,000 g for 10 ...the platelet-poor plasma ...
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From 3D to 3D: isolation of mesenchymal stem/stromal cells into a three-dimensional human platelet lysate matrix
... Adipose tissue represents an easily accessible and eth- ically not questionable source of MSCs. The cells are usually isolated by mechanical dissociation and subse- quent enzymatic digestion of the tissue with ...
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Adipocyte Derived Mesenchymal Stromal Cell and Platelet Lysate: Ideal Cell and Supplement for the Treatment of Immune Inflammatory Diseases?
... (2012) Platelet Lysate from Whole Blood-Derived Pooled Platelet Concentrates and Apheresis-Derived Platelet Concentrates for the Isolation and Expansion of Human Bone Marrow Mesenchymal ...
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Human Platelet lysate as a Xeno Free Alternative of Fetal bovine serum for the In Vitro Expansion of Human Mesenchymal Stromal Cells
... are platelet derived growth factor (PDGF) and transforming growth factor β ...the platelet granules are fibroblast growth factors (FGF-1 and FGF-2), 17 insulin-like growth factor-1 (IGF-1), 18,19 epidermal ...
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Platelet lysate as a novel serum-free media supplement for the culture of equine bone marrow-derived mesenchymal stem cells
... of platelet-derived products, such as platelet lysate (PL), obtained following the lysis of platelets from platelet concentrates or platelet-rich plasma (PRP) as a media supplement for ...
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A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement
... risk. Platelet units may contain virus (that is, HIV, cytomega- lovirus, and so forth) or parasites (that is, Tripanozoma cruzii in our country), which may be transmitted to a po- tential recipient if cells are ...
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Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches
... Fetal bovine serum (FBS) has been traditionally used in vitro as a source of growth factors (GFs) and other elements essential for cell adhesion and proliferation [13]. However, its use has raised several concerns in re- ...
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