Pluripotency/stem cell markers
Generation and characterization of transgene-free human induced pluripotent stem cells and conversion to putative clinical-grade status
... standard stem cell markers (NANOG, OCT4, and SOX2) as indicated by quantitative PCR analysis (Figure ...standard pluripotency markers (Figure ...
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Original Article YAP down-regulated its target CTGF to maintain stem cell pluripotency in human ovarian cancer stem-like cells
... distinct cell origin and may arise from the differentiated cells that have acquired self-renewing capacity but not the transforma- tion of normal stem ...cancer stem-like cells by tumor-bearing mice ...
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Identification and characterization of epithelial cells derived from human ovarian follicular fluid
... Epithelial cell cultures were established from 18 ...germ-line stem cell markers, such as octamer-binding transcription factor 4 (OCT4), NANOG, and DEAD box polypeptide 4 ...epithelial ...
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Hütz, Katharina Antonia (2013): The role of the transcription factor SOX2 in tumorigenesis and development of the stomach. Dissertation, LMU München: Medizinische Fakultät
... for pluripotency in ES cells (see Introduction ...to pluripotency in adult stem cells, evidences have accumulated that SOX2 plays an important role in these cells ...adult stem cells in the ...
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Expression of pluripotency markers in human granulosa cells after embryonic stem cell extract exposure and epigenetic modification
... specific markers and express Oct4 but not the other pluripotent markers ...the pluripotency markers was down-regulated and the number of the cells that expressed these markers was few ...
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Modeling inherited metabolic disorders of the liver using human induced pluripotent stem cells
... iPS cell library from patients with IMDs Dermal fibroblasts were obtained from skin biopsies from 7 individuals with a range of IMDs and from 3 healthy controls (20 human iPS cell lines, 5 diseases; Table 1 ...
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Involvement of gelsolin in TGF-beta 1 induced epithelial to mesenchymal transition in breast cancer cells
... the markers of stem cell pluripotency ...mesenchymal cell markers ...epithelial cell marker ...ling cell proliferation and motility, we conducted GSN overexpression ...
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Transposable elements in the mammalian embryo: pioneers surviving through stealth and service
... drive cell type and developmental stage-specific expres- sion, particularly during early embryogenesis when the genome becomes demethylated ...embryonic stem cells ...in pluripotency markers ...
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ISG15 predicts poor prognosis and promotes cancer stem cell phenotype in nasopharyngeal carcinoma
... SUNE1 cell lines with ISG15 overexpression (Figure 4A) and performed a series of in vitro experiments with these ...CSC markers by real time PCR analysis. Expression levels of pluripotency-associated ...
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Hydractinia, a pioneering model for stem cell biology and reprogramming somatic cells to pluripotency
... These stem cells, now known as interstitial cells (i-cells), are thought to remain pluripotent throughout their ...own stem cells and repopu- lated with allogeneic mutant donor stem cells, it was ...
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Restoring miR122 in human stem-like hepatocarcinoma cells, prompts tumor dormancy through Smad-independent TGF-β pathway
... BCLC9 cell line was established from a well- differentiated human HCC [12] and they show a stem cell phenotype characterized by gene and protein expression of a pool of pluripotency ...
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Original Article Human amniotic fluid stem cells support undifferentiated propagation and pluripotency of human embryonic stem cell without b-FGF in a density dependent manner
... AFS cell layer culture ...AF-type cell colonies are more abundant (> 60%) than F-type cell colonies ...AF-type cell colonies, we selected this type cells for further ...
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The Role of Cytoplasmic Polyadenylation Element Binding Protein-2 (CPEB-2) in Breast Cancer
... MicroRNAs (miRNAs) are small regulatory RNA molecules (19-24 nucleotides) that are transcribed from DNA but not translated into protein. miRNAs act to down-regulate gene expression at the post-transcriptional level by ...
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Brca1 breast tumors contain distinct CD44+/CD24 and CD133+cells with cancer stem cell characteristics
... some cell lines showed surviving floating colonies that formed compact sphe- roids after 3 to 4 weeks of ...each cell line was tested by plating cells in limiting dilution from 500 to 1 cell/well on ...
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Expression of intestinal stem cell and cancer stem cell markers in submucosal invasion and its prognostic significance in gastric cancers
... (Advanced Cell Diagnostics, ...per cell; score 1: 1 to 3 dots per cell (visible at ×20–40 magnification); score 2: 4 to 10 dots per cell and no or very few dot clusters (visible at ×20–40); ...
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Gene methylation of human ovarian carcinoma stromal progenitor cells promotes tumorigenesis
... targeting stem/progenitor cells has emerged as a novel potential approach to battle ovarian cancer ...target stem/progenitor cells [35, 36], and the durable inhibition of tumor growth (lasting for at least ...
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Regulation of germ layer formation by pluripotency factors during embryogenesis
... the pluripotency regulatory network ...of pluripotency in recent years and can act as substitutes of Oct4, Klf4 or Nanog in reprogramming or in maintenance of pluripotency ...‘nonclassical’ ...
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Pluripotency of induced pluripotent stem cells
... first cell fate ...inner cell mass (ICM) and the rest differentiate into trophectoderm that forms the extraem- bryonic tissues and supports embryonic ...tissue-specific stem cells, including ...
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Heterochromatin Protein 1β (HP1β) has distinct functions and distinct nuclear distribution in pluripotent versus differentiated cells
... iPSCs and ESCs, in stark contrast to the characteristic heterochromatic foci found in the nuclei of differentiated MEFs (Fig. 5a, c; see Figure S6a in Additional file 6 for shorter exposure). This phenomenon was also ...
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PRMT5 is essential for the maintenance of chondrogenic progenitor cells in the limb bud
... widespread cell death that includes mesodermal progenitor cells that have begun to precociously differentiate into cartilage ...embryonic stem cells also require PRMT5 for maintaining pluripotency, ...
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