Pluripotent stem Cell-derived cardiomyocytes
Metabolomics Identifies Metabolic Markers of Maturation in Human Pluripotent Stem Cell-Derived Cardiomyocytes
... Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) hold immense clinical potential and recent studies have enabled generation of virtually pure hPSC-CMs with high efficiency ...
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GLUT4 expression and glucose transport in human induced pluripotent stem cell-derived cardiomyocytes
... Induced pluripotent stem cell derived cardiomyocytes (iPSC-CM) have the potential to trans- form regenerative cardiac medicine and the modelling of cardiac ...of cardiomyocytes ...
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Susceptibility of murine induced pluripotent stem cell-derived cardiomyocytes to hypoxia and nutrient deprivation
... of cardiomyocytes (CMs) into injured myocardium has been shown to improve contractile func- tion in animal models of heart disease ...induced pluripotent stem cells (iPSCs) and improvements in ...
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Qualitative transcriptional signatures for evaluating the maturity degree of pluripotent stem cell-derived cardiomyocytes
... Background: Pluripotent stem cell-derived cardiomyocytes (PSC-CMs) are widely used models for regenerative medicine and disease ...embryonic stem cell (ESC) samples, we ...
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Friedreich's ataxia induced pluripotent stem cell derived cardiomyocytes display electrophysiological abnormalities and calcium handling deficiency
... Intracellular Ca 2+ was measured by microfluorimetry (Ionoptix MA, USA). Cells were loaded with the Ca 2+ fluorescent dye FURA-2/AM (5 ȝM, 10 min incubation at room temperature, Molecular Probes). They were placed in a ...
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Considerations for pre-clinical models and clinical trials of pluripotent stem cell-derived cardiomyocytes
... diomyopathy, cell homing of administered CD34+ cells was investigated by labeling a fraction of the cells with 99mTc-hexamethylpropylenamine and performing single- photon emission computed tomography imaging 2 and ...
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Induced pluripotent stem cell-derived cardiomyocytes for cardiovascular disease modeling and drug screening
... iPSC-derived cardiomyocytes for cell replacement therapy As the human heart is largely unable to replenish lost cardiomyocytes, studies have been conducted to examine ways to restore cardiac ...
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Cardiac repair in a murine model of myocardial infarction with human induced pluripotent stem cell-derived cardiomyocytes
... of cell therapy is the low survival rate and engraftment after transplantation ...direct cell replacement, the release of exosomes from iPSC-CMs represents a new type of iPSC-based cardio- myogenesis ...
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Uninterrupted monitoring of drug effects in human induced pluripotent stem cell derived cardiomyocytes with bioluminescence Ca2+ microscopy
... induced pluripotent stem cells (hiPSC) [1, 2], which can now be differentiated into virtually all cell types in ...Patient derived disease-specific hiPSCs recapitulate many disease phenotypes ...
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An integrated characterization of contractile, electrophysiological, and structural cardiotoxicity of Sophora tonkinensis Gapnep. in human pluripotent stem cell-derived cardiomyocytes
... Cardiotoxicity is still one of the primary factors restricting the development of drugs. Drug development is a time-consuming and expensive process, and past statistics show that up to 90% of compounds that passed ...
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Perspectives on the Use of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Biomedical Research
... to cardiomyocytes, and assessed for hy- pertrophy (Carvajal-Vergara et ...hiPSC- cardiomyocytes displayed a higher degree of sarcomeric organization and nuclear NFATC4 localization and were larger than ...
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Tissue-engineered cardiac patch seeded with human induced pluripotent stem cell derived cardiomyocytes promoted the regeneration of host cardiomyocytes in a rat model
... Explanted cardiac patches were fixed in 4% para- formaldehyde, embedded in paraffin, sliced (5 μm thick sections), and stained with Hematoxylin and Eosin (H&E). H&E staining was used for cell counting. One ...
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Induced pluripotent stem cell–derived cardiomyocytes in studies of inherited arrhythmias
... iPS- derived cardiomyocytes using microelectrode recordings from spontaneously beating cells at 56–70 days after the formation of the ...iPS- derived myocytes is the impressive heterogeneity in the ...
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Physical developmental cues for the maturation of human pluripotent stem cell-derived cardiomyocytes
... single cell studies as well as most two-dimensional and three- dimensional culture systems usually require enzymatic ...substrate, cell density and timing of experimentation are critical ...whole ...
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Developmental cues for the maturation of metabolic, electrophysiological and calcium handling properties of human pluripotent stem cell-derived cardiomyocytes
... in cell communication and signal transduction as well as host defense responses in adult and fetal CMs but not in hESC-CMs ...of cell develop- ment, highlighting the developing processes that hESCs are ...
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Cardiotoxicity evaluation using human embryonic stem cells and induced pluripotent stem cell-derived cardiomyocytes
... at 24 h post administration. On the other hand, high concentration (10 μmol/L) of haloperidol abolished the spontaneous beating of hESC-CMs and no self-recovery was observed during the investigation period. Calculation ...
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Combining patient proteomics and in vitro cardiomyocyte phenotype testing to identify potential mediators of heart failure with preserved ejection fraction
... Background: Heart failure with ejection fraction (HFpEF) is a syndrome resulting from several co‑morbidities in which specific mediators are unknown. The platelet proteome responds to disease processes. We hypothesize ...
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Biomechanical regulation of in vitro cardiogenesis for tissue-engineered heart repair
... on pluripotent stem cell-derived cardiomyocytes, may benefit from staggered tonic loading protocols to simulate viscoelastic properties of the prenatal and postnatal myocardial ...
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Selection of human induced pluripotent stem cells lines optimization of cardiomyocytes differentiation in an integrated suspension microcarrier bioreactor
... 55]. In our studies, a high throughput platform (Optio- QUANT™) developed by Ternion Bioscience, Singapore, was used to image and quantify subtle changes of mem- brane potentials in hPSC-/hiPSC-derived CMs, based ...
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Disruption of histamine/H1R signaling pathway represses cardiac differentiation and maturation of human induced pluripotent stem cells
... into cardiomyocytes according to a classical protocol ...beating cardiomyocytes initially appeared on days 8–10 (GiWi; ...beating cardiomyocytes to appear (Fig. 2d). Cell clone morph- ology ...
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