PMA-differentiated THP-1 cell
Induction of IFNα or IL 12 depends on differentiation of THP 1 cells in dengue infections without and with antibody enhancement
... [32], PMA-differentiated THP-1 cells (2 x 10 5 cells/ml) were incubated with DEN- 2 virus with and without DEN immune sera at MOI of 1 at 37°C for 60 ...infected ...
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III. Ion Channel Expression in PMA-differentiated Human THP-1 Macrophages
... mia cell line, THP-1, after differentiation induced by ...related cell lines (Gallin, 1991). One advantage of using the THP-1 cell line over primary human monocytes is a ...
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Ebola Virus Exploits a Monocyte Differentiation Program To Promote Its Entry
... ferentiated THP-1 cells, and differentiated PMA-treated THP-1 ...of 1. FIG 4 THP-1 cells become permissive for EBOV entry after PMA-mediated ...of ...
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Changes on lysosomal compartment during PMA induced differentiation of THP 1 monocytic cells: Influence of type I and type IV collagens
... model, THP-1 cells undergo, si- multaneously, the consequences of both PMA and sub- strate interaction, represented, alternatively, by the two kinds of ...only PMA stimulation (adhesion to ...
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USP18 (UBP43) Abrogates p21-Mediated Inhibition of HIV-1
... in PMA- differentiated THP-1 ...and PMA-differentiated THP-1 cells in the presence or absence of type I IFN, which is a known inducer of p21 (21, 54, ...in ...
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Propolis exerts an anti-inflammatory effect on PMA-differentiated THP-1 cells via inhibition of purine nucleoside phosphorylase
... An increase in the levels of NADH in the P-C-treated samples also supports the proposal that the treatment is promoting the TCA cycle. Increased flux through the TCA cycle could support the increase in levels of ATP and ...
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Myxoma Virus Lacking the Pyrin-Like Protein M013 Is Sensed in Human Myeloid Cells by both NLRP3 and Multiple Toll-Like Receptors, Which Independently Activate the Inflammasome and NF-κB Innate Response Pathways
... vMyxM013-KO. THP-1 cells were transfected with siRNAs for nontargeting control, RIG-I, MDA5, MAVS, or MyD88, differentiated for 12 to 18 h with PMA, and then infected with vMyxM013-KO virus ...
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Adenovirus Type 5 Rupture of Lysosomes Leads to Cathepsin B-Dependent Mitochondrial Stress and Production of Reactive Oxygen Species
... by PMA-differentiated THP-1 ...in PMA- differentiated THP-1 cells ...Ad5 cell entry required to elicit ROS from infected cells, we infected the ...
8
Phagocytosis of hemozoin by RAW 264.7 cells, but not THP-1 cells, promotes infection by Leishmania donovani with a nitric oxide-independent mechanism
... in THP-1 cells might have been due to a reduced internalization of the malarial pigment and/or its degradation, cellular phagocytosis of HZ and BH was assessed overtime in PMA-differentiated ...
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Curcumin inhibits EMMPRIN and MMP 9 expression through AMPK MAPK and PKC signaling in PMA induced macrophages
... in PMA in- duced THP-1 cells, we first measured the protein level of phosphorylated AMPKα in THP-1 differentiated ...of THP-1 cells [37,38], and PMA induced ...
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Effect of melittin on metabolomic profile and cytokine production in PMA-differentiated THP-1 cells
... the cell cytoplasm through exchange with malate, and the citrate is then converted to oxaloacetate and acetyl-CoA, using ...the cell membrane, as confirmed by several previous findings ...(Table 1 ...
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Adenovirus Membrane Penetration Activates the NLRP3 Inflammasome
... inflammatory cell necrosis. (A) PMA-differentiated THP-1cntrl or THP-1nlrp3KD cells were rested in serum-free medium for 2 h, primed with PAM3CSK4 for 2 h or not, and treated with ...
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Metabolomic profiling of the immune stimulatory effect of eicosenoids on PMA-differentiated THP-1 cells
... The TCA cycle intermediates, such as succinate and citrate, are critical in M1 macrophage activation and are positively associated with inflammation [38]. High levels of succinate were observed in the present study in ...
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High resolution analysis of aberrant regions in autosomal chromosomes in human leukemia THP 1 cell line
... in THP-1 cells (Figure 4(B), ...cancer cell lines and ...and cell lines excluding the THP-1, and it was revealed that a majority of the cell lines carried mutations or ...
7
Characterization of Simian Immunodeficiency Virus SIVSM/Human Immunodeficiency Virus Type 2 Vpx Function in Human Myeloid Cells
... is cell type specific, and it is observed not only in cognate viruses but also in primate and nonprimate lentiviruses as well as gammaretroviruses ...with cell-type-specific ...
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Rhinovirus Replication in Human Macrophages Induces NF-κB-Dependent Tumor Necrosis Factor Alpha Production
... expression. THP-1-derived M were lysed directly into sodium dodecyl sulfate (SDS) sample buffer ...a 1/5,000 dilution of rabbit anti-3C RV protease antibody (provided by Svetlana Amineva, University ...
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Posttranslational Regulation of IL-23 Production Distinguishes the Innate Immune Responses to Live Toxigenic versus Heat-Inactivated Vibrio cholerae
... human THP-1 cells with wild-type ...in THP-1 monocytes in response to live ...the cell culture stimulation experiment, and error bars represent standard errors of the ...in ...
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Curcumin induces the apoptosis of human monocytic leukemia THP 1 cells via the activation of JNK/ERK Pathways
... approach. PMA, a PKC activator, is known to promote the differentiation of immature THP-1 mono- cytic cells to mature THP-1 ...in PMA- treated THP-1 ...in ...
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Screening of compounds toxicity against human Monocytic cell line-THP-1 by flow cytometry
... The worldwide rapid increase in bacterial resistance to numerous antibiotics requires on-going development of new drugs to enter the market. As the development of new antibiotics is lengthy and costly, early monitoring ...
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Investigation of the immunostimulatory effects of some New Zealand honeys and characterization of an active component : a thesis submitted in partial fulfilment of the requirements for the degree of Master of Technology in Food Technology at Massey University, Albany, New Zealand
... Beta glucans (β-glucans) are potent biological response modifiers (BRM) that activate the immune response via macrophage cells to yield various therapeutic effects relating to defense reactions against infection (Novak ...
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