PMA-differentiated THP-1 cells
Metabolomic profiling of the immune stimulatory effect of eicosenoids on PMA-differentiated THP-1 cells
... Immune cells utilise five metabolic pathways: glycolysis, the TCA cycle, the pentose phosphate pathway (PPP), fatty acid synthesis and amino acid metabolism ...(PMA)-differentiated ...
26
Effect of melittin on metabolomic profile and cytokine production in PMA-differentiated THP-1 cells
... Abstract: Melittin, the major active peptide of honeybee venom (BV), has potential for use in adjuvant immunotherapy. The immune system response to different stimuli depends on the secretion of different metabolites from ...
21
Propolis exerts an anti-inflammatory effect on PMA-differentiated THP-1 cells via inhibition of purine nucleoside phosphorylase
... macrophage cells by LPS [38] following targeted metabolic profiling of individual pathways, such as amino acids [39], carbohydrates [40] and lipids [41], provided a broad determination of the pathogenic mechanism ...
21
USP18 (UBP43) Abrogates p21-Mediated Inhibition of HIV-1
... in PMA- differentiated THP-1 ...and PMA-differentiated THP-1 cells in the presence or absence of type I IFN, which is a known inducer of p21 (21, 54, ...in ...
19
Phagocytosis of hemozoin by RAW 264.7 cells, but not THP-1 cells, promotes infection by Leishmania donovani with a nitric oxide-independent mechanism
... in THP-1 cells might have been due to a reduced internalization of the malarial pigment and/or its degradation, cellular phagocytosis of HZ and BH was assessed overtime in ...
40
Induction of IFNα or IL 12 depends on differentiation of THP 1 cells in dengue infections without and with antibody enhancement
... [32], PMA-differentiated THP-1 cells (2 x 10 5 cells/ml) were incubated with DEN- 2 virus with and without DEN immune sera at MOI of 1 at 37°C for 60 ...infected ...
9
Adenovirus Type 5 Rupture of Lysosomes Leads to Cathepsin B-Dependent Mitochondrial Stress and Production of Reactive Oxygen Species
... by PMA-differentiated THP-1 ...in PMA- differentiated THP-1 cells ...infected cells, we infected the macrophage-like PMA-differentiated ...
8
Myxoma Virus Lacking the Pyrin-Like Protein M013 Is Sensed in Human Myeloid Cells by both NLRP3 and Multiple Toll-Like Receptors, Which Independently Activate the Inflammasome and NF-κB Innate Response Pathways
... myeloid cells (Fig. 8). Since MYXV replicates as well in many human cancer cells as it does in rabbit cells, but is essentially nonpathogenic to any nonlago- morph host such as mice or humans, it is ...
13
Changes on lysosomal compartment during PMA induced differentiation of THP 1 monocytic cells: Influence of type I and type IV collagens
... of THP-1 monocytic cell line was ...The cells in these three phases of differen- tiation were obtained by using 6, 30 e 60 nM ...of differentiated status expression, in relation to the ...
11
Adenovirus Membrane Penetration Activates the NLRP3 Inflammasome
... (A) PMA-differentiated THP-1cntrl or THP-1nlrp3KD cells were rested in serum-free medium for 2 h, primed with PAM3CSK4 for 2 h or not, and treated with control medium or infected with ...
10
III. Ion Channel Expression in PMA-differentiated Human THP-1 Macrophages
... immune cells have been ...in THP-1 cells were ...of cells expressing I DR from 7% to 92%, but concluded that this 13-fold increase in the number of cells expressing I DR might ...
17
Ebola Virus Exploits a Monocyte Differentiation Program To Promote Its Entry
... line THP-1 was used in experiments to address the role of differentiation in EBOV infection of mono- ...these cells do not spontaneously dif- ferentiate in culture. However, THP-1 ...
14
Curcumin inhibits EMMPRIN and MMP 9 expression through AMPK MAPK and PKC signaling in PMA induced macrophages
... in PMA in- duced THP-1 cells, we first measured the protein level of phosphorylated AMPKα in THP-1 differentiated ...of THP-1 cells [37,38], and ...
10
Inflammasome Antagonism by Human Parainfluenza Virus Type 3 C Protein
... in THP-1 cells during inflammasome activation in the absence and presence of the HPIV3 C ...protein-expressing THP-1 cells were treated with either LPS or LPS plus ...these ...
17
TLR4-NOX2 axis regulates the phagocytosis and killing of Mycobacterium tuberculosis by macrophages
... the cells in the LPS treatment group, different concentrations of LPS (final concentrations of 10, 100, 1000, and 10,000 ng/mL) were added to stimulate the cells for 24 ...the cells into the tubes of ...
9
Interferon-Inducible CD169/Siglec1 Attenuates Anti-HIV-1 Effects of Alpha Interferon
... in THP-1 ...in THP-1 cells. Since VSV-G-pseudotyped HIV-1 has been extensively used in studies for characterizing ISG functions against HIV-1, care should be taken in ...
20
Latent Kaposi's Sarcoma-Associated Herpesvirus Infection of Monocytes Downregulates Expression of Adaptive Immune Response Costimulatory Receptors and Proinflammatory Cytokines
... B cells, monocytes, and epithelial and endothelial ...B cells and endothelial cells (7, 12, 27, ...KSHV- THP-1 cells predominantly expressed a latent viral gene profile with low ...
8
Possible role of microparticles in neuroimmune signaling of microglial cells
... exposing THP-1 cells to MPs (10 μg protein/mL) isolated from THP-1 cells that had been stimulated with IFN- γ alone or in combination with LPS for 48 ...LPS-stimulated ...
11
Treating cancer stem cells and cancer metastasis using glucose-coated gold nanoparticles
... different. Circulation is not the only reason behind metas- tasis. Cancer cells survive and grow by angiogenesis, the process of building a new blood supply. Cancer can spread almost anywhere in the body, but the ...
13
Hepatitis B virus differentially suppresses myelopoiesis and displays tropism for immature hematopoietic cells.
... A greater degree of virus expression was seen in THP-1 cells than in HL-60 cells, and the percentage of THP-1 cells expressing HBsAg or HBcAg was three to four times greater than that fo[r] ...
7