polo-like kinase 2
Polo-like kinase 2 acting as a promoter in human tumor cells with an abundance of TAp73
... Materials and methods: Head and neck squamous cell carcinoma cell lines and osteosarcoma cell lines were used as natural models of the different expression levels of TAp73. Phosphoryla- tion predictor software Scansite ...
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Characterization of the Sak kinase interaction with cyclin B1 and Gadd45a
... Polo-like Kinases Function and Members 1 The Structure of the Polo-like Kinases 2 Plks Expression and Kinase Activity 4.. Plks Localization 5.[r] ...
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Putting a bit into the polo-box domain of polo-like kinase 1
... Plk1: a cancer cell-selective anticancer drug target Consistent with the important role of Plk1 in regulating various mitotic events, Plk1 overexpression is thought to promote neoplastic transformation of human cells ...
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Polo-Like Kinase 1 as a Target for Human Cytomegalovirus pp65 Lower Matrix Protein
... Several recent studies have shown that the kidnapping of cellular proteins is a widespread viral strategy. Besides the com- mon case of cell membrane proteins trapped in viral envelopes, a number of cell proteins of ...
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Pooled shRNA screen for sensitizers to inhibition of the mitotic regulator polo-like kinase (PLK1)
... GSK461364A treatment was for three days followed by a washout of the compound and recovery in growth medium for three to seven days. GSK461364A was removed from plates by three washes with 20% FBS- supplemented complete ...
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Co-inhibition of polo-like kinase 1 and aurora kinases promotes mitotic catastrophe
... completely inhibited using relatively high concentrations of the drugs. As described above, inhibition of PLK1 induced a metaphase arrest (Figure 2; Supplementary Video 3). Inhibition of AURKA causes defects in ...
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Polo-Like Kinase 1 Is Involved in Hepatitis C Virus Replication by Hyperphosphorylating NS5A
... (human kinase and phosphatase genes) subset of VSV-G pseudotyped lentiviruses expressing distinct shRNA was employed for ...investigation. Polo-like kinase 1 (Plk1), a serine/ threonine ...
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Polo like kinase confers MPF autoamplification competence to growing Xenopus oocytes
... Plx1 kinase activity (Karaiskou et ...Cdc2 kinase activity and analyzing Cdc25 phosphatase and Myt1 kinase phosphorylation states, or directly the Tyr15 phosphorylation of Cdc2 ...H1 kinase ...
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Targeting polo-like kinase 1, a regulator of p53, in the treatment of adrenocortical carcinoma
... membranes in 5 % BSA (1× TBST + 5 % BSA) overnight at 4 °C. Primary antibodies to MDM2 (AbCam, Cam- bridge, MA, USA) and p53 (AbCam, Cambridge, MA) at a dilution of 1:500 were added to the membranes in 1 % blocking ...
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Polo-Like Kinase Guides Cytokinesis in Trypanosoma brucei through an Indirect Means
... G 2 /M transition (1, 37), metaphase/anaphase transition (2), anaphase release (20), mitotic exit (18), and initiation of cytokinesis (6, 14, 45) in yeasts and ...two Polo boxes, to specific targets ...
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Exploration of Mutation and DNA Methylation of Polo Like Kinase 1 (PLK1) in Colorectal Cancer
... for the in vitro studies [19] [20]. Among the cell lines, SW48 is known to be ra- diosensitive T84 is radioresistant while radiosensitivity of HCT116 and Colo- 320DM remain intermediate [21] [22] [23] [24]. These allow ...
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MICROWAVE ASSISTED SYNTHESIS AND MOLECULAR MODELING STUDIES OF SOME NOVEL POLO-LIKE KINASE 1 INHIBITORS AS ANTICANCER AND ANTI-INFLAMMATORY AGENTS
... A data set of 19 compounds (5a – 5s) of substituted N-(4-oxo-2- phenyl-1, 3-thiazolidin-3-yl) benzamide for in vitro anti- inflammatory activity was used for the present QSAR study. There is sufficient range of ...
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Impact of Polo-like kinase 1 inhibitors on human adipose tissue- derived mesenchymal stem cells
... The Polo-like kinase (Plk) family is a group of highly conserved serine/threonine ...N-terminal kinase domain and the C-terminal regulatory Polo-box domain (PBD), which offer multiple ...
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Hyper-activation of Aurora kinase a-polo-like kinase 1-FOXM1 axis promotes chronic myeloid leukemia resistance to tyrosine kinase inhibitors
... CML progression is driven by multiple genetic and epigen- etic events, including amplification of BCR-ABL1, increased activity of BCR promoter, impaired activity of PP2A, inhib- ition of SRC homology region 2 ...
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Retusenol Potentially Inhibits Putative Drug Targets for Tuberculosis, Cardiovascular Diseases, Cancer and HIV: A Reverse Docking Study
... Table 2 and illustrated in ...b) polo-like kinase 1, and c) ...and polo-like kinase 1 was almost exclusively van der Waals in ...with polo-like kinase ...
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A TAT-conjugated peptide inhibitor of polo-like kinase 1 for in vivo tumor imaging
... imaging, 2 was injected into HeLa tumor-bearing mice xenograft to determine the tumor-tar- geting and diagnosis ...cm 2 /sr at 3 h, 2.62 ± 0.26 × 10 8 p/s/cm 2 /sr at 6 h, and ...p/s/cm 2 /sr ...
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Polo-like kinase 4 at the nexus of epigenetic modifications and the DNA damage signaling network
... Low-dose decitabine (5-aza-2'-deoxycytidine) has been approved for the treatment of myelodysplastic and leukemic patients. A deoxycytosine analogue, decitabine (DAC), is able to reverse epigenetic mutations by ...
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The activity regulation of the mitotic centromere-associated kinesin by Polo-like kinase 1
... Immunofluorescence staining was performed as previously described [19]. Briefly, cells transfected with MCAK constructs were fixed for 8-10 min with methanol at -20°C or 4% paraformaldehyde containing 0.1% Triton X-100 ...
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Polo-like kinase 1 (Plk1): an Unexpected Player in DNA Replication
... binds directly to ACS to initiate DNA replication. How- ever, even in a simple system such as this, the ACS is not sufficient in itself to predict the origin; the exact location of ACS on the chromosome is also a ...
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The balance of Polo-like kinase 1 in tumorigenesis
... Polo-like kinases (Plks) are serine/threonine kinases with an N-terminal kinase domain and a C-terminal polo-box domain. The domain structure of this family is evolution- ary conserved from ...
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