Polyclonal Antibody
Production, characterization, and cross-reactivity of a polyclonal antibody against Arabidopsis TARGET OF RAPAMYCIN
... purified polyclonal α‑TOR antibody successfully detected endogenous TOR proteins in wild‑type Arabidopsis and TOR orthologous in major crop plants, including tomato, maize, and ...α‑TOR antibody is ...
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Synthesis of hapten and preparation of specific polyclonal antibody with high affinity for lenalidomide, the potent drug for treatment of multiple myeloma
... the antibody to the analyte of interest is the limiting factor in the validity of the ...specific antibody with high affinity for LND was ...a polyclonal antibody that can specifically ...
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Polyclonal Antibody against Recombinant Nucleoprotein of the Influenza A Virus (H1N1); Production and Purification
... as polyclonal antibody production against it, diagnostic and differential tests can be developed which can be used at the time of the epidemic onset as well as basic research and development for prevention ...
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Production, purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virus
... the antibody possesses high specificity and ...anti-tgK polyclonal antibody has the potential to produce subunit vaccines and the functions of neutralizing DEV and anti-DEV infection because of its ...
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Polyclonal antibody against the DPV UL46M protein can be a diagnostic candidate
... Background: The duck plague virus (DPV) UL46 protein (VP11/12) is a 739-amino acid tegument protein encoded by the UL46 gene. We analyzed the amino acid sequence of UL46 using bioinformatics tools and defined the main ...
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Polyclonal antibody directed against human RANTES ameliorates disease in the Lewis rat adjuvant induced arthritis model
... This is a very different pattern from what we observed with the RANTES antibody treatment. In our study anti-RANTES treatment ended on day 7. By day 11 one would predict that all of the rabbit polyclonal ...
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Arabidopsis Thaliana AXR4 Recombinant Protein Expression for Polyclonal Antibody Production
... generate polyclonal antibody. The antibody recognized a protein of approximately 54 kDa in the membrane fractions prepared from Arabidopsis ...AXR4 antibody will contribute towards elucidation ...
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A methodological approach for production and purification of polyclonal antibody against dog IgG
... adjuvant. Polyclonal IgG were purified by ion exchange chromatography and then the purified antibody was labeled with horse radish peroxidase ...This antibody could be a useful tool for future dog ...
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Development and characterization of polyclonal antibody against human kappa light chain in rabbit
... Human immunoglobulin G (IgG) purification. Blood samples were taken from patients referred to the Imam Reza Hospital (Tabriz, Iran) with their informed consent (No. TBZMED.REC.1394.457). Peripheral blood samples were ...
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Evaluation of Human Anti IgG Polyclonal Antibody Production Conjugated with Peroxidase in Egg Yolk
... purified antibody was boiled and 10, 20, and 40 µl of the same were added to columns 2, 3, and 4, respectively, using a Hamilton syringe; the first column was dedicated to the protein ...
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Characterization and Epitope Mapping of the Polyclonal Antibody Repertoire Elicited by Ricin Holotoxin-Based Vaccination
... toward cultured cells. Each representative phage was preincubated with the antibodies at its highest possible concentration before it was mixed with ricin and added to the cell culture. Under these conditions, the ...
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Resistance to Vesicular Stomatitis Virus Infection Requires a Functional Cross Talk between the Eukaryotic Translation Initiation Factor 2α Kinases PERK and PKR
... goat polyclonal antibody to PERK (T-19; Santa Cruz), rabbit polyclonal antibody to phosphothreonine 980 of PERK (Cell Signaling), rabbit polyclonal antibody to PERK (H-300; Santa ...
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Expression and Characterization of a Novel Structural Protein of Human Cytomegalovirus, pUL25
... a polyclonal antibody was raised against a prokaryotic pUL25 fusion protein in order to investigate the biosynthesis and localization of the UL25 product (pUL25) during HCMV replication in human ...
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The Gsp-1 genes encode the wheat arabinogalactan peptide
... a polyclonal antibody to their GSP preparation and western blotting of aqueous extracts of our lines with this antibody showed a clear decrease in binding to a component of mass about 18,000 in the ...
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Application of an indirect immunofluorescence test for detection of Mycoplasma pneumoniae in respiratory exudates
... We prepared polyclonal antibody specific to Mycoplasma pneumoniae and examined the conditions influencing the ability of an indirect immunofluorescence test to detect the specific antige[r] ...
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The epidermolysis bullosa acquisita antigen (type VII collagen) is present in human colon and patients with crohn's disease have autoantibodies to type VII collagen
... a polyclonal antibody to the NC1 domain of type VII collagen diluted in TTBS with 1% bovine serum albumin (BSA; 1:2000) (Chen et al, ...peroxidase-conjugated antibody to rabbit IgG at a dilution of ...
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One-step indirect competitive ELISA development for dexamethasone determination in chicken
... rabbits. Polyclonal antibody was subsequently obtained and ...The antibody exhibited significantly cross-reactivity with some of DEX structural ...
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Comparison of nine commercial immunoassays for the detection of rotavirus in fecal specimens
... Kits evaluated included two monoclonal antibody-based enzyme immunoassays EIAs Rotaclone and Pathfinder Rotavirus, three polyclonal antibody-based EIAs Rotavirus Immunoassay, Rotazyme II[r] ...
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Siglec-15: a potential regulator of osteoporosis, cancer, and infectious diseases
... Using polyclonal antibody, Siglec-15 was found to be expressed in a subset of the cells that express DC-SIGN (a macrophage/dendritic cell marker) in human spleen and lymph nodes ...
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Recombinant neuraminidase pseudotyped baculovirus: a dual vector for delivery of Angiotensin II peptides and DNA vaccine
... Recombinant neuraminidase-AngII pseudotyped bacu- lovirus had been shown to successfully display AngII peptides on its surface for effectively presenting these peptide antigens to the immune system. This rBV-NA- AngII ...
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