Polyketide synthase
Identification of polyketide synthase 13 inhibitor: Pharmacophore-based virtual screening and molecular dynamics simulation
... The obtained ligands from the virtual screening using ZINCPharmer were then subjected to molecular docking on polyketide synthase 13 (Pks13) active site employing iDock software (Li et al., 2012). The ...
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Mining for nonribosomal peptide synthetase and polyketide synthase genes revealed a high level of diversity in the sphagnum bog metagenome
... Müller, Christina A., Oberauner-Wappis, Lisa, Peyman, Armin, Amos, Gregory C. A., Wellington, E. M. H. (Elizabeth M. H.), 1954-, Berg, Gabriele and Müller, V.. (2015) Mining for nonribosomal peptide synthetase and ...
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Crystal structure of the putative cyclase IdmH from the indanomycin nonribosomal peptide synthase/polyketide synthase
... peptide synthase/ polyketide synthase (NRPS/PKS) followed by a number of ‘tailoring’ steps to form the two ring systems that are present in the mature ...in polyketide-tailoring ...
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Functional Dissection of a Multimodular Polypeptide of the Pikromycin Polyketide Synthase into Monomodules by Using a Matched Pair of Heterologous Docking Domains
... pikromyin polyketide synthase (PKS) in Streptomyces vene- zulae is comprised of a loading module and six extension mod- ules, which generate the corresponding 14-membered macro- lactone ...
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Isolation of a polyketide synthase gene from Dothistroma pini : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Molecular Biology at Massey University, Palmerston North, New Zealand
... Aspergillus parasiticus polyketide synthase gene pksA, a homolog of Aspergillus nidulans wA, is required for aflatoxin Bl biosynthesis.. Molecular and General Genetics.[r] ...
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The Polyketide Synthase Gene pks4 of Trichoderma reesei Provides Pigmentation and Stress Resistance
... In this study, we functionally characterized the role of the polyketide synthase PKS4 in T. reesei. pks4 belongs to the nonre- ducing clade I of fungal pks-encoding genes, which includes genes associated ...
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Unexpected link between polyketide synthase and calcium carbonate biomineralization
... I polyketide synthase (PKS) (Figure ...latipes polyketide synthase ...ketoacyl synthase (KS), acyl transferase (AT), dehydratase (DH), ketoreductase (KR), and acyl carrier protein ...
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The polyketide backbone of thiolactomycin is assembled by an unusual iterative polyketide synthase
... thiotetronate polyketide assembly unex- pectedly involving an iterative, rather than modular, ...to polyketide synthase multi- enzymes throughout chain assembly, a recently developed chemical ...
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Functional Analysis of the Polyketide Synthase Genes in the Filamentous Fungus Gibberella zeae (Anamorph Fusarium graminearum)
... Identification of PKS genes. Fifteen putative PKS genes were identified by analyzing the raw genomic sequence. PKS genes were designated using the previously assigned designa- tions (34) except where function has been ...
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The insect pathogen Serratia marcescens Db10 uses a hybrid non ribosomal peptide synthetase polyketide synthase to produce the antibiotic althiomycin
... synthetase-polyketide synthase (NRPS-PKS) assembly line (Alb4/5/6), tailoring enzymes (Alb2/3) and an export/resistance protein (Alb1), and suggested that the machinery assembled althiomycin or a related ...
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Identification of a Polyketide Synthase Gene Responsible for Ascochitine Biosynthesis in Ascochyta fabae and Its Abrogation in Sister Taxa
... The polyketide-derived secondary metabolite ascochitine is produced by species in the Didymellaceae family, including but not restricted to Ascochyta species pathogens of cool-season food ...a polyketide ...
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Molecular detection of type II polyketide synthase genes in Cuban soils
... Distribution and diversity of type II polyketide synthase genes in soil populations of actinornycetes colonising Cuban soils.. Portrait of an Organism: The Genetic Analysis oj' Streptomy[r] ...
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A crotonyl CoA reductase carboxylase independent pathway for assembly of unusual alkylmalonyl CoA polyketide synthase extender units
... YCCs assemble other unusual PKS extender units. BLAST searches identified two other polyketide biosynthetic gene clusters that contain genes encoding an YCC b-subunit with the same specificity-conferring residues as ...
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A Streptomyces coelicolor host for the heterologous expression of Type III polyketide synthase genes
... The well-characterized germicidin synthase gene of S. coelicolor was chosen initially to validate the utility of M1317 as a host for the expression of Type III PKS genes. gcs (sco7221), which is capable of ...
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Chemical chain termination resolves the timing of ketoreduction in a partially reducing iterative type I polyketide synthase
... In the present study, chemical probes were used to elucidate the programming of a PR-PKS which, unlike other related enzymes, is integrated into a multimodular assembly line. Not- withstanding this specific feature, our ...
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Isolation of a polyketide synthase gene from Dothistroma pini : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Molecular Biology at Massey University, Palmerston North, New Zealand
... 1 .6 AIMS AND OBJECTIVES The availability of cloned at1atoxin genes from Asperg illus parasiticus, and other fungal species which contain genes for polyketide production, provided an opp[r] ...
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Designed biosynthesis of 25-methyl and 25-ethyl ivermectin with enhanced insecticidal activity by domain swap of avermectin polyketide synthase
... could not lead to the drastic decrease in the total amount of avermectins and ivermectin. Indeed, the use of alter- native domains and modules from PKSs of structurally related polyketides is considered as an efficient ...
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Biochemical and biophysical characterisation of the quartromicin polyketide synthase
... Type I PKS systems may either be categorised as iterative or modular. 80 Iterative PKS systems contain a module with the ability to catalyse repetitive elongations and modi- fications. In each catalytic cycle, different ...
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Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production
... by polyketide synthase (PKS) have been well interpreted despite that some highly controlled synthetic processes still remained ...the polyketide chain has finished, a thioesterase (TE) hydrolyses the ...
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Elucidating molecular mechanisms of actinobacterial polyketide alkaloid biosynthesis
... III polyketide synthase (PKS) enzymology and atypical non- ribosomal peptide synthetase (NRPS) biochemistry in germicidin 15 and coelichelin 16 biosynthesis, ...
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