Polyketide synthase (PKS)
Crystal structure of the putative cyclase IdmH from the indanomycin nonribosomal peptide synthase/polyketide synthase
... synthetase/ polyketide synthase (NRPS/PKS; Li et ...predicted PKS modules to yield the linear nonribosomal peptide–polyketide hybrid natural product 2 ...the polyketide is still ...
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The insect pathogen Serratia marcescens Db10 uses a hybrid non ribosomal peptide synthetase polyketide synthase to produce the antibiotic althiomycin
... synthetase-polyketide synthase (NRPS-PKS) assembly line (Alb4/5/6), tailoring enzymes (Alb2/3) and an export/resistance protein (Alb1), and suggested that the machinery assembled althiomycin or a ...
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Unexpected link between polyketide synthase and calcium carbonate biomineralization
... I polyketide synthase (PKS) (Figure ...latipes polyketide synthase ...I PKS has a set of distinct enzymatic domains that individually catalyze a series of reactions, to produce a ...
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Functional Dissection of a Multimodular Polypeptide of the Pikromycin Polyketide Synthase into Monomodules by Using a Matched Pair of Heterologous Docking Domains
... pikromyin polyketide synthase (PKS) in Streptomyces vene- zulae is comprised of a loading module and six extension mod- ules, which generate the corresponding 14-membered macro- lactone ...this ...
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The polyketide backbone of thiolactomycin is assembled by an unusual iterative polyketide synthase
... I PKS by recruiting three successive propionate units and catalyzing three cycles of chain ...thiotetronate polyketide assembly unex- pectedly involving an iterative, rather than modular, ...
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Identification of a Polyketide Synthase Gene Responsible for Ascochitine Biosynthesis in Ascochyta fabae and Its Abrogation in Sister Taxa
... The polyketide-derived secondary metabolite ascochitine is produced by species in the Didymellaceae family, including but not restricted to Ascochyta species pathogens of cool-season food ...a polyketide ...
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Identification of polyketide synthase 13 inhibitor: Pharmacophore-based virtual screening and molecular dynamics simulation
... Polyketide synthase 13 (Pks13), together with the acyl- AMP ligase (FadD32), is involved in the biosynthesis of long- chain (C60-90) α-branched-β-hydroxylated fatty acid, mycolic acid, one of the main lipid ...
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Molecular detection of type II polyketide synthase genes in Cuban soils
... Distribution and diversity of type II polyketide synthase genes in soil populations of actinornycetes colonising Cuban soils.. Portrait of an Organism: The Genetic Analysis oj' Streptomy[r] ...
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Isolation of a polyketide synthase gene from Dothistroma pini : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Molecular Biology at Massey University, Palmerston North, New Zealand
... Aspergillus parasiticus polyketide synthase gene pksA, a homolog of Aspergillus nidulans wA, is required for aflatoxin Bl biosynthesis.. Molecular and General Genetics.[r] ...
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Mining for nonribosomal peptide synthetase and polyketide synthase genes revealed a high level of diversity in the sphagnum bog metagenome
... Müller, Christina A., Oberauner-Wappis, Lisa, Peyman, Armin, Amos, Gregory C. A., Wellington, E. M. H. (Elizabeth M. H.), 1954-, Berg, Gabriele and Müller, V.. (2015) Mining for nonribosomal peptide synthetase and ...
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A Streptomyces coelicolor host for the heterologous expression of Type III polyketide synthase genes
... III PKS genes. Deletion of the resident Type III PKS genes could potentially increase precursor pool levels and prevent undesirable interference with bio- synthesis encoded by heterologous Type III ...
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Chemical chain termination resolves the timing of ketoreduction in a partially reducing iterative type I polyketide synthase
... In the present study, chemical probes were used to elucidate the programming of a PR-PKS which, unlike other related enzymes, is integrated into a multimodular assembly line. Not- withstanding this specific ...
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A crotonyl CoA reductase carboxylase independent pathway for assembly of unusual alkylmalonyl CoA polyketide synthase extender units
... modular PKS via a rational activation of a silent biosynthetic gene cluster in Streptomyces ambofaciens ATCC23877 (refs ...stambomycin PKS chain elongation modules are predicted to employ widely-used ...
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The Polyketide Synthase Gene pks4 of Trichoderma reesei Provides Pigmentation and Stress Resistance
... of polyketide syn- thases (PKSs) in Trichoderma ecophysiology is not well ...the PKS repertoire in ...11 PKS-encoding genes were found in the ...
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Functional Analysis of the Polyketide Synthase Genes in the Filamentous Fungus Gibberella zeae (Anamorph Fusarium graminearum)
... 15 PKS genes result in mutants with increased growth on a rich, sterile me- ...of PKS genes by Kroken et al. (34), the only fungi that did not have PKS genes in the genome were the yeasts ...
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Biochemical and biophysical characterisation of the quartromicin polyketide synthase
... M5 was successfully immobilised (20,000 RU, sodium acetate, pH 6.3) and responses to 20 µM samples of each ACP in its apo form were measured (Figure 5.13). Successful re- generation of M5 to its unbound state was ...
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Designed biosynthesis of 25-methyl and 25-ethyl ivermectin with enhanced insecticidal activity by domain swap of avermectin polyketide synthase
... milbemycin PKS may be more suitable for swapping AveDH2-KR2 than the domains from other PKS reported previously [6, 8, ...in polyketide chain transfer and elon- gation [10, ...
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Dissecting and engineering of the TetR family regulator SACE_7301 for enhanced erythromycin production in Saccharopolyspora erythraea
... Saccharopolyspora erythraea, a Gram-positive industrial actinomycetes, produces a valuable macrolide antibiotic erythromycin A (Er-A). Er-A and its semi-synthetic deriv- atives, such as clarithromycin, azithromycin, ...
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Investigations of carbocyclisation reactions in prodiginine alkaloid biosynthesis
... modular polyketide synthase assembly lines, as the predicted product of the PKS encoded by cluster 15 does not bear a significant resemblance to ECO-02301 (see ...
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Optimizing Dothistroma septosporum infection of Pinus radiata and the development of red band disease : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Genetics at Massey University, Palmerston North, New Zealand
... The dotD gene encodes a 322 amino acid polypeptide that has putative thioesterase enzymatic activity with homology to polyketide synthase pks genes involved in AF/ST biosynthesis Bradsha[r] ...
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