polymerase activity
G Protein Pathway Suppressor 1 Promotes Influenza Virus Polymerase Activity by Activating the NF κB Signaling Pathway
... ABSTRACT Influenza virus relies heavily on cellular machinery to replicate in host cells. Therefore, to better understand the influenza virus life cycle, it is important to identify which host proteins are involved and how ...
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Host and Strain Specific Regulation of Influenza Virus Polymerase Activity by Interacting Cellular Proteins
... virus polymerase activity in human ...virus polymerase, NP, and/or vRNP were analyzed by network ...virus polymerase activity. For assays for viral polymerase phenotypes, siRNAs ...
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Investigation of Influenza Virus Polymerase Activity in Pig Cells
... vitro polymerase assay reflected a difference of replication in the context of infectious virus, we gen- erated a set of recombinant viruses by reverse ...Bavaria/1/77 polymerase and NP genes or encoded the ...
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RNA-directed DNA polymerase activity of reticuloendotheliosis virus: characterization of the endogenous and exogenous reactions.
... Antiserum to the DNA polymerase of an REV group virus spleen necrosis virus inhibited both endogenous and exogenous DNA polymerase activity of REV, whereas antiserum to the Rous sarcoma [r] ...
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Deoxyribonucleic Acid Polymerase Activity Associated with a Plasma Particulate Fraction from Patients with Chronic Lymphocytic Leukemia
... Evidence that the enzyme activity was contained in a particle came from the observations that detergent was able, in both cases, to increase DNA polymerase activity and that the enzyme f[r] ...
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A Small-RNA Enhancer of Viral Polymerase Activity
... trimeric polymerase, truncated HA cRNA, and synthetic svRNAs ...RdRp activity, as well as synthesis of full-length ...baseline activity of the RdRp can result in small RNA ...modulate ...
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Ribonuclease-Sensitive Deoxyribonucleic Acid Polymerase Activity in Uninfected Rat Cells and Rat Cells Infected with Rous Sarcoma Virus
... To determine whether the template for the endogenous DNA polymerase activity in extracts of RSV-infected rat cells was similar to RNA from virions of RSV, a standard polymerase reaction [r] ...
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Adaptation of Borna Disease Virus to New Host Species Attributed to Altered Regulation of Viral Polymerase Activity
... the activity of the viral polymerase complex? Important clues came from studies aimed at measuring the strength of the interactions between the various viral polymerase ...that polymerase ...
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RNA Polymerase Activity Associated with Bacteriophage φ6
... Experiments to determine if the radioactivity incorporated in the assay mixture was associated with the phage particle could not be conducted since the short heat treatment used to stimu[r] ...
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Poly(A) Polymerase Activity in Reovirus
... Properties of oligoA producta Percent TreatmentPecn acid soluble Pancreatic DNase 0 Ti + pancreatic RNase 0.3 M NaCl 0.01 M NaCl U2 RNase KOH' 0 51 97 >99 a Virions were incubated for 4 [r] ...
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Polymerase Activity of Pichinde Virus
... Approximately 600 ml of extracellular fluid containing 7 x 107 to 10' PFU of Pichinde virus per ml was harvested, purified, and tested for RNA-dependent DNA polymerase and RNAdependent R[r] ...
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Identification of poliovirus polypeptide P63 as a soluble RNA-dependent RNA polymerase.
... To determine whether both p63 and NCVP2 are required for polymerase activity, we further purified the soluble polymerase and analyzed the viral proteins associated with the purified enzy[r] ...
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Conserved Features of the PB2 627 Domain Impact Influenza Virus Polymerase Function and Replication
... in polymerase activity ...abolished polymerase activity in human and avian ...in polymerase activity resulted from changes in the levels of these ...displayed activity ...
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Induction of Poxvirus Ribonucleic Acid Polymerases
... Polymerase activity of infected cell extracts treated with mercaptoethanol 1% final concentration but no detergent a; activity of extracts made to 1% with both NP40 and mercaptoethanol 0[r] ...
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Hepatitis C virus in vitro replication is efficiently inhibited by acridone Fac4
... Figure 3. Fac4 possible mechanisms of action: A) Intercalation assay, evaluating Fac4 dsRNA intercalation property. Doxorrubicin (Doxo) was used as positive control. B) Evaluation of Fac4 effect over T7 RNA ...
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Macromolecular Content of Inclusions Produced by a Canine Adenovirus
... The level of DNA polymerase activity in exponentially growing infected and uninfected whole cells was similar, but in cells in stationary phase the enzyme activity of infected cells was [r] ...
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Specific Serological Relationships Among Partially Purified DNA Polymerases of Avian Leukosis-Sarcoma Viruses, Reticuloendotheliosis Viruses, and Avian Cells
... For example, IgGRSV-RAV-0 did not neutralize TDSNV DNA polymerase activity, and IgG-TDSNV only slightly neutralized RSV-RAV-0 DNA polymerase activity, whereas RSV-RAV-0 DNA polymerase wa[r] ...
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Virus of Pekin ducks with structural and biological relatedness to human hepatitis B virus.
... Endogenous polymerase activity was monitored by mixing a portion of each sucrose gradient fraction with an equal volume of 2x polymerase assay mixture, containing 0.15 M NaCl, and then p[r] ...
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Adenovirus DNA synthesis in vitro in an isolated complex.
... They did not report inhibition studies on DNA synthesis in intact complexes or on the DNA polymerase activity in extracts of the complexes, and it may be that the a-polymerase of the inf[r] ...
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Distinguishing cytomegalovirus, mycoplasma, and cellular DNA polymerases.
... The time course of appearance of salt- NH42SO4, the virus-induced DNA polymerase stimulated DNA polymerase activity in total- activity in total-cell extracts can be readily discell extra[r] ...
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