Polymerase chain reaction amplification
Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene
... Detection of methicillin-resistant Staphylococcus aureus (MRSA) using polymerase chain reaction amplification. Staphylo-[r] ...
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Detection of Babesia bigemina infected carriers by polymerase chain reaction amplification
... Analysis of PCR-amplified DNA products of bovine blood from experimentally infected cattle (group A) by agarose gel electrophoresis (A) and Southern blot hybridization with digoxige- nmn[r] ...
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Detection of enterotoxigenic Escherichia coli after polymerase chain reaction amplification with a thermostable DNA polymerase
... The direct identification of enterotoxigenic Escherichia coli from clinical specimens was examined by using the polymerase chain reaction (PCR) for amplifying the heat-labile toxin (LT) [r] ...
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Reverse transcription and polymerase chain reaction amplification of rRNA for detection of Helicobacter species
... pylon detection by RT-PCR assay, the production of cDNA was performed in a total volume of 30 pl containing PCR buffer with the above concentrations of each deoxynucleotide, 180 ng of HP[r] ...
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Polymerase chain reaction amplification of the constant and variable regions of the Bacteroides nodosus fimbrial gene
... Nucleotide sequence of the pilin gene from Bacteroides nodosus strain 238 (serogroup G). Nucleic Acids Res[r] ...
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Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens
... method was applied to 10 rotavirus reference strains, including all 6 known human serotypes (serotypes 1, 2, 3, 4, 8, and 9), and to 34 stool specimens previously serotyped by an enzyme [r] ...
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Detection of Babesia bovis carrier cattle by using polymerase chain reaction amplification of parasite DNA
... bovis carrier cattle were detected 86% of the time (range, 66 to 100%) when they were tested 11 times, while with microscopic examination of thick blood smears, the same carrier cattle w[r] ...
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Detection of Theileria sergenti infection in cattle by polymerase chain reaction amplification of parasite specific DNA
... Analysis of the gene encoding immunodominant piroplasm surface proteins of Theileria sergenti and Theilena buffeli by nucleotide sequencing and polymerase chain reaction. Identification [r] ...
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Detection of hepatitis B virus DNA in serum by polymerase chain reaction amplification and microtiter sandwich hybridization
... We have developed a microtiter sandwich hybridization assay for the detection of polymerase chain reaction (PCR)-amplified hepatitis B virus (HBV) sequences.. This assay utilizes an enzy[r] ...
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Rapid epidemiologic analysis of cytomegalovirus by using polymerase chain reaction amplification of the L S junction region
... The gel electrophoretic patterns of DNA bands, or PCR profiles, produced by amplification with the L-S primers were unique for epidemiologically unrelated strains and laboratory referenc[r] ...
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Discrimination among thermophilic Campylobacter species by polymerase chain reaction amplification of 23S rRNA gene fragments
... Specific detection of Campy- lobacter jejuni and Campylobacter coli by using polymerase chain reaction. The genus Campylobacter[r] ...
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Detection by polymerase chain reaction amplification of human herpesvirus 6 DNA in peripheral blood of patients with exanthem subitum
... The polymerase chain reaction was used to detect human herpesvirus 6 (HHV-6) DNA in peripheral blood mononuclear cells from patients with exanthem subitum.. Amplified products were detec[r] ...
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DNA sequence variation within maize and melon: observations from polymerase chain reaction amplification and direct sequencing.
... lower. The reason for this observed difference in genetic variation is not well understood. There ap- pears to be no correlation between this disparity and genome size [r] ...
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Enzymatic amplification of human cytomegalovirus sequences by polymerase chain reaction
... (152 bp) observed after PCR with all three HCMV-infected cell cultures (FF/AD169 and two FF cultures infected with clinical isolates) but not with the uninfected FF cells (Fig. 7B) indic[r] ...
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Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction
... sequences conserved throughout the eubacterial kingdom, we amplified bacterial 16S ribosomal DNA.. sequences with the polymerase chain reaction.[r] ...
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Detection of Trypanosoma cruzi by DNA amplification using the polymerase chain reaction
... (I'lZi, since these four isolates were obtained from patients in widely separated areas (southern Brazil, Chile, Texas, and northern Brazil, respectively). In contrast, when DNAs from so[r] ...
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Detection of Coxiella burnetti by DNA amplification using polymerase chain reaction
... burnetii isolates by PCR in tissue culture material and in direct patient specimens (heart valves) using oligonucleotide primers derived from the superoxide dismutase gene se-.. quence o[r] ...
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Specific Amplification of Ribosomal Sequences Using Comultiplex Polymerase Chain Reaction
... Amplification of DNA sequences from ribosomal DNA (rDNA) was tested as a specific and sensitive method for the detection of small numbers of Toxoplasma gondii tachyzoite cells.. We appli[r] ...
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Enzymatic amplification of platelet specific messenger RNA using the polymerase chain reaction
... direct amplification, using the polymerase chain reaction, of platelet-derived mRNA in amounts sufficient to permit detailed analysis, such as restriction mapping and nucleotide ...
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Detection of hepatitis C virus ribonucleic acid in the serum by amplification with polymerase chain reaction
... We analyzed 15 amplified cDNA seg- ments from 15 different patients, and found that a portion of viral cDNA nucleotide sequences showed 68-78% homology with those of the prototype HCV re[r] ...
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