Polymerase Chain Reaction For Amplification Of
Optimization of polymerase chain reaction for amplification of microsatellite markers of wildcat Felis silvestris Schreber, 1777
... OF POLYMERASE CHAIN REACTION FOR AMPLIFICATION OF MICROSATELLITE MARKERS OF WILDCAT Felis silvestris Schreber, 1777 Lucija Jaša-Šangulin, 6982/BT Abstract: Genomic purity of European wildcat ...
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Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene
... Detection of methicillin-resistant Staphylococcus aureus (MRSA) using polymerase chain reaction amplification. Staphylo-[r] ...
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Enzymatic amplification of platelet specific messenger RNA using the polymerase chain reaction
... direct amplification, using the polymerase chain reaction, of platelet-derived mRNA in amounts sufficient to permit detailed analysis, such as restriction mapping and nucleotide ...
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Unusual amplification in polymerase chain reaction for a plasmid containing an insert derived from bovine genomic DNA
... Nucleotide Sequence; Palindrome 1. INTRODUCTION The saliva of various animals contains proline-rich proteins which may play important roles in prevention of mineral precipitation, protection of dietary and digestive ...
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Exosomes molecular diagnostics: Direct conversion of exosomes into the cDNA for gene amplification by two-step polymerase chain reaction
... into polymerase chain reaction (PCR) tubes, and then lysed by adding lysing buffer in the same PCR tube, with subsequent direct conversion of isolated RNA into ...PCR amplification were ...
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Enzymatic amplification of human cytomegalovirus sequences by polymerase chain reaction
... (152 bp) observed after PCR with all three HCMV-infected cell cultures (FF/AD169 and two FF cultures infected with clinical isolates) but not with the uninfected FF cells (Fig. 7B) indic[r] ...
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Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction
... sequences conserved throughout the eubacterial kingdom, we amplified bacterial 16S ribosomal DNA.. sequences with the polymerase chain reaction.[r] ...
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Detection of Trypanosoma cruzi by DNA amplification using the polymerase chain reaction
... (I'lZi, since these four isolates were obtained from patients in widely separated areas (southern Brazil, Chile, Texas, and northern Brazil, respectively). In contrast, when DNAs from so[r] ...
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Detection of Babesia bigemina infected carriers by polymerase chain reaction amplification
... Analysis of PCR-amplified DNA products of bovine blood from experimentally infected cattle (group A) by agarose gel electrophoresis (A) and Southern blot hybridization with digoxige- nmn[r] ...
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Detection of Coxiella burnetti by DNA amplification using polymerase chain reaction
... burnetii isolates by PCR in tissue culture material and in direct patient specimens (heart valves) using oligonucleotide primers derived from the superoxide dismutase gene se-.. quence o[r] ...
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Specific Amplification of Ribosomal Sequences Using Comultiplex Polymerase Chain Reaction
... Amplification of DNA sequences from ribosomal DNA (rDNA) was tested as a specific and sensitive method for the detection of small numbers of Toxoplasma gondii tachyzoite cells.. We appli[r] ...
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Detection of enterotoxigenic Escherichia coli after polymerase chain reaction amplification with a thermostable DNA polymerase
... The direct identification of enterotoxigenic Escherichia coli from clinical specimens was examined by using the polymerase chain reaction (PCR) for amplifying the heat-labile toxin (LT) [r] ...
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Reverse transcription and polymerase chain reaction amplification of rRNA for detection of Helicobacter species
... pylon detection by RT-PCR assay, the production of cDNA was performed in a total volume of 30 pl containing PCR buffer with the above concentrations of each deoxynucleotide, 180 ng of HP[r] ...
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Polymerase chain reaction amplification of the constant and variable regions of the Bacteroides nodosus fimbrial gene
... Nucleotide sequence of the pilin gene from Bacteroides nodosus strain 238 (serogroup G). Nucleic Acids Res[r] ...
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Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens
... method was applied to 10 rotavirus reference strains, including all 6 known human serotypes (serotypes 1, 2, 3, 4, 8, and 9), and to 34 stool specimens previously serotyped by an enzyme [r] ...
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Detection of hepatitis C virus ribonucleic acid in the serum by amplification with polymerase chain reaction
... We analyzed 15 amplified cDNA seg- ments from 15 different patients, and found that a portion of viral cDNA nucleotide sequences showed 68-78% homology with those of the prototype HCV re[r] ...
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Sequence analysis and amplification by polymerase chain reaction of a cloned DNA fragment for identification of Mycobacterium tuberculosis
... From this sequence two pairs of primers were synthesized (MtbAB and MtbCD), ranging from 18 to 22 nucleotides, for use in polymerase chain reactions (PCRs) with DNA from six reference st[r] ...
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Detection of Babesia bovis carrier cattle by using polymerase chain reaction amplification of parasite DNA
... bovis carrier cattle were detected 86% of the time (range, 66 to 100%) when they were tested 11 times, while with microscopic examination of thick blood smears, the same carrier cattle w[r] ...
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Detection of Theileria sergenti infection in cattle by polymerase chain reaction amplification of parasite specific DNA
... Analysis of the gene encoding immunodominant piroplasm surface proteins of Theileria sergenti and Theilena buffeli by nucleotide sequencing and polymerase chain reaction. Identification [r] ...
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Detection of hepatitis B virus DNA in serum by polymerase chain reaction amplification and microtiter sandwich hybridization
... We have developed a microtiter sandwich hybridization assay for the detection of polymerase chain reaction (PCR)-amplified hepatitis B virus (HBV) sequences.. This assay utilizes an enzy[r] ...
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