polymerase chain reaction assay
The magnetic immuno polymerase chain reaction assay for direct detection of salmonellae in fecal samples
... The Magnetic Immuno Polymerase Chain Reaction Assay for Direct Detection of Salmonellae in Fecal Samples MYRA N.. VERDONK,1,2 AND JAN VERHOEF' Eijkman Winkler Laboratory for Medical Micr[r] ...
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Identification of Pentatrichomonas hominis in feline fecal samples by polymerase chain reaction assay
... Pentatrichomonas hominis is considered to be a commensal protozoan of the vertebrate digestive tract. On the basis of light microscopic examination of feces, some investigators presumptively identified P. hominis as a ...
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Clinical evaluation of a new polymerase chain reaction assay for detection of Chlamydia trachomatis in endocervical specimens
... A clinical evaluation of the Amplicor polymerase chain reaction PCR assay for the detection of Chlamydia trachomatis in endocervical swabs Roche Molecular Systems, Branchburg, N.J.. This[r] ...
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Polymerase chain reaction assay of parvovirus B19 DNA in clinical specimens
... In a control panel of 100 serum samples from individuals with other infections, only 1 serum sample was found positive by PCR, and this was also found positive by dot hybridization.. Thi[r] ...
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Polymerase chain reaction assay for avian polyomavirus
... Lanes 2, 3, 5, 7, and 9 contain the amplified products from purified DNA samples from CEFs infected with BFDV, pooled organ tissue from a nestling budgerigar, purified viral DNA from a l[r] ...
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Diagnosis of Foot-and-Mouth Disease Virus by Real Time Reverse Transcription Polymerase Chain Reaction Assay in Iran
... immunosorbent assay (ELISA) methods have been considered as diagnostic tests for FMD ...transcription polymerase chain reaction (RT- PCR) methods are used to detect and identity FMDV ...
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Polymerase chain reaction assay for detection of human cytomegalovirus
... Urine specimens from six renal transplant recipients which had been previously characterized as positive for the presence of HCMV by either ELISA, hybridization assay with a biotinlabele[r] ...
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Direct detection of Mycobacterium tuberculosis in clinical specimens by DNA amplification
... A polymerase chain reaction assay was developed for the amplification of a 336-bp repetitive fragment in the chromosome of Mycobacterium tuberculosis.. The assay is specific for M.[r] ...
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Kudoa neurophila in striped trumpeter : identification, diagnostic development and histopathology
... Chapter 4 Diagnostic polymerase chain reaction assay to detect Kudoa neurophila Myxozoa: Multivalvulida in a striped trumpeter, Latris lineata hatchery and the epizootiology of the disea[r] ...
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Accuracy of a Polymerase Chain Reaction-based Assay for Detection of Pneumococcal Bacteremia in Children
... The results of the present study suggest that the PCR assay which we developed lacks the character- istics of a good screening test. Possible causes for false positives include background contamination from ...
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Evaluation of a Polymerase Chain Reaction (PCR) Assay for the diagnosis of Trichomonas Vaginalis Infection.
... The first set of primers (TVA5/ TVA6) amplified the Trichomonas vaginalis DNA from all the three samples i.e., undiluted supernatant, undiluted concentrate and 1/10 diluted concentrate. The expected band size for the ...
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Application of PCR to multiple specimen types for diagnosis of cytomegalovirus infection: comparison with cell culture and shell vial assay
... Development and clinical significance of a diagnostic assay based on the polymerase chain reaction for detection of human cytomegalovirus DNA in blood samples from immunocompromised pati[r] ...
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Polymerase chain reaction analyses identify two distinct classes of Borrelia burgdorferi
... A specific and sensitive assay for the Lyme disease spirochete Borrelia burgdorferi using the polymerase chain reaction.. Enzymatic amplification of beta-globin genomic sequences and res[r] ...
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Development of a simple and rapid sipA-directed PCR assay for serovar-independent specific identification of Salmonella enterica bacteria
... reliable polymerase chain reaction (PCR) assay using Salmonella sipA gene-specific primers as a molecular identification method for all Salmonella enterica ...PCR assay showed 100% ...
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Kudoa neurophila in striped trumpeter : identification, diagnostic development and histopathology
... Polymerase chain reaction diagnostic assay to detect Pentacapsula neurophila Multivalvulida, the cause of brain disease in cultured striped trumpeter Latris lineata Forster 1801.. Pathol[r] ...
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Carbapenem and Fluoroquinolone Resistance in Multidrug Resistant Pseudomonas aeruginosa Isolates from Al-Zahra Hospital, Isfahan, Iran
... minimum inhibitory concentration (MICs). The E-test is a relatively new agar diffusion-based method for the quantitative evaluation of bacterial susceptibilities. We compared the sensitivities of the results obtained ...
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Predominant expression of beta 1 adrenergic receptor in the thick ascending limb of rat kidney Absolute mRNA quantitation by reverse transcription and polymerase chain reaction
... Beta 1- and beta 2-adrenergic receptor (beta-ARs) expression in the thick ascending limb of rat kidney was studied at the level of mRNA and receptor coupling to adenylyl cyclase. Absolute quantitation of beta 1- and beta ...
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Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction
... A rapid and sensitive polymerase chain reaction PCR-based assay for detection of Chlamydia trachomatis in cervical specimens is described.. This assay consists of i sample preparation wh[r] ...
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Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis
... as polymerase chain reaction (PCR), which amplify a specific nucleic acid sequence, have been developed over the past ...the assay and the still not-far-enough advanced port- able devices for ...
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Specific detection of Campylobacter jejuni and Campylobacter coli by using polymerase chain reaction
... Development of a routine detection assay for Campylobacter jejuni and Campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction PCR.. An oligonucleot[r] ...
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