Polymerase chain reaction (PCR)-based methods
Comparison of three nonradioisotopic polymerase chain reaction based methods for detection of human immunodeficiency virus type 1
... Three nonradioisotopic polymerase chain reaction (PCR)-based detection techniques were evaluated for sensitivity and specificity in detecting human immunodeficiency virus type 1 (HIV-1) [r] ...
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THE advent of the polymerase chain reaction in the
... chloroform based extractions) and PCR, often via ‘‘primer- ...the methods were in generating sequenc- es that were ultimately useful for tree inference particularly in studies that did not include a ...
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Polymerase chain reaction–based assays for the diagnosis of human brucellosis
... necessary. Polymerase chain reaction (PCR) technique offers a sensitive and specific way of detecting Brucella ...various methods based on PCR for labora- tory diagnosis of human ...
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Improved methods using the reverse transcriptase polymerase chain reaction to detect tumour cells
... Although RT-PCR detection of circulating tumour cells has been shown to increase the sensitivity of small-volume disease detec- tion, the clinical significance of detecting low levels of disease remains unclear, and ...
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RAPID DETECTION OF Salmonella IN SHRIMP BY POLYMERASE CHAIN REACTION [Deteksi Cepat Salmonella pada Udang dengan Polymerase Chain Reaction]
... conventional methods which take more than 5 ...detection methods have been developed. Detection method of Salmonella spp. based on PCR has been reported by Hyeon et ...
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Polymerase chain reaction-based active surveillance of MRSA in emergency department patients
... With regard to the BD GeneOhm MRSA assay, previous studies have confirmed that this assay is both specific and sensitive. 6,16,17 The sensitivity of the BD GeneOhm MRSA assay in the present study was relatively low, ...
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Accuracy of a Polymerase Chain Reaction-based Assay for Detection of Pneumococcal Bacteremia in Children
... Methods. Children evaluated at the Children’s Hos- pital of Pittsburgh who were having blood drawn for culture had an additional 2 to 3 mL of blood (from the same sampling) obtained and placed in a sodium citrate ...
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Polymerase chain reaction based detection of Trypanosoma cruzi DNA in serum
... disease detected in babies I and II, parasitological methods yielded negative results on the very day of birth (Fig. 4A, lanes 1 and 5, respectively), yet the babies were positive by the[r] ...
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Detection of human herpesvirus 8 by quantitative polymerase chain reaction: development and standardisation of methods
... these methods were ideal for our assays, nor are HHV-8 quantitative controls available within Australia, an attempt was made to import plasmids from international ...
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Antiadenoviral effects of ganciclovir in types inducing keratoconjunctivitis by quantitative polymerase chain reaction methods
... Discussion In this study, ganciclovir had a significant inhibitory effect in a dose-dependent manner against all selected types of HAdV – HAdV3, 4, 8, 19a, and 37 – which induce epidemic keratoconjunctivitis. Ganciclovir ...
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Polymerase chain reaction ribotyping of Clostridium difficileisolates in Qatar: a hospital based study
... Methods Patients From October 2011 to August 2012, consecutive pa- tients with suspected CDI admitted to Hamad General Hospital and Al-Khor Hospital, Qatar, were included in the study. Both of these hospitals are ...
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Development of a polymerase chain reaction based diagnosis of Trichomonas vaginalis
... primer pair TVA5-TVA6 uniformly detected the 102-bp target sequence in 15 clinical isolates of T.. vaginalis, includ- ing some isolates from diverse geographic sources (Fig.[r] ...
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Polymerase chain reaction: trenches to benches
... The application of molecular diagnostic techniques to the diagnosis of infectious disease is based on the identification of unique "signature sequences" in the DNA or RNA of a pa[r] ...
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Polymerase chain reaction on a viral nanoparticle
... 3 An alternative approach to the construction of materials from biological components has been to exploit an existing fibrous chassis that has been evolved within nature, which concentrates on functionalization of a ...
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Polymerase Chain Reaction Ppt Presentation
... or polymerase chain reaction is slidesfinder account? They are hybridized and polymerase ppt presentation on all of these can only available for optimizing pcr shares the purpose of ...
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Detection of Clostridium tyrobutyricum using cultivation and biochemical methods and polymerase chain reaction
... In this work, two cultivation methods for detection of butyric acid producing clostridia in raw and pas- teurized milk and in cheese samples were compared. The results show that tube method is suitable for route ...
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Deletion of DNA sequences of using a polymerase chain reaction based approach
... the polymerase and presence of restriction ...of polymerase, 5 U of Dpn I, 5 U of T4 ligase, and cells for transformation are required per reaction, what can be found in most molecular biology ...
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Detection of leptospires in urine by polymerase chain reaction
... Under experimental conditions an amplification was obtained with DNA of Leptospira interrogans of some serovars belonging to serogroup sejroe.. However, very little or no amplification w[r] ...
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Polymerase chain reaction for detection of Mycobacterium tuberculosis
... The sensitivity of the amplification performed with target DNAs extracted by using the lysis procedure described above was estimated by using serial dilutions of a culture containing app[r] ...
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