polymerase chain reaction technique.
Nested polymerase chain reaction technique for the detection of Gpc3 and Afp mRNA in liver cancer micrometastases.
... first chain took place using a reaction mixture comprising: 1 µL dithiothreitol, 4 µL 5X buffer, 200 U (1 µL) Moloney murine leukemia virus reverse transcriptase (M-MLV RT), 18-20 ...
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Assessment of tubercle bacilli in various organs by staining and polymerase chain reaction technique
... AFB. Polymerase chain reaction was done in 20 cases of which 18 (90%) were positive rendering this method as very sensitive and specific method for detection of tubercle bacilli than staining ...
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Isolation and Molecular Identification of Salmonella Typhi Using Polymerase Chain Reaction (PCR) Technique
... this reaction, surface protein linked to lip id II, is then incorporated into the cell wall envelope via the transpeptidation and transglycosylation reactions of peptidoglycan ...catalyzed reaction is the ...
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SCIENTIFIC CONCEPTS OF POLYMERASE CHAIN REACTION (PCR)
... of Polymerase Chain Reaction (PCR) technology in forensic science and molecular ...laboratory technique for amplifying a specific sequence of a minute amount of Deoxyribonucleic acid (DNA) ...
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MOLECULAR ANALYSIS OF MLL GENE REARRANGEMENTS IN CHILDHOOD ACUTE LEUKEMIA: A HOSPITAL BASE STUDY
... reverse-transcriptase polymerase chain reaction (PMRT-PCR) technique was performed for molecular ...molecular technique is willing to come in routine molecular ...
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The polymerase chain reaction in the charaterisation and diagnosis of lymphomas
... light chain restric tio n in tissu e sections.^® However th is technique cannot prove a clonal relatio n sh ip betw een neoplastic cells in serial biopsy specim ...
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Polymerase chain reaction on a viral nanoparticle
... the polymerase chain (PCR) ...absorbance technique that is only able to produce a signal if chromophores within a scaffold are aligned with respect to one ...
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Different DNA methylation patterns detected by the Amplified Methylation Polymorphism Polymerase Chain Reaction (AMP PCR) technique among various cell types of bulls
... From this work, we proved that the AMP PCR techni- que could generate methylation-associated fingerprints from different cells and tissues obtained from Holstein bulls. The technique could be used as a screening ...
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Development of real time polymerase chain reaction for detection of Salmonella typhimurium and Salmonella enteritidis in fish
... direct technique. This technique is very useful to cut the time that consumed by overnight enrichment for the PCR method or several days for biochemical testing with conventional ...
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Polymerase chain reaction – restriction fragment length polymorphism analysis for the differentiation of mycobacterial species in bronchial washings
... Decontamination of specimens was done using standard NaOH/Na citrate technique [17]. Decontaminated specimens were inoculated onto Lowenstein-Jensen (LJ) media and Middle brook 7H-10 and cultures were observed for ...
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Detection of Enhanced Green Fluorescent Protein DNA in Pink Bollworm through Polymerase Chain Reaction
... This technique will be a helpful adjunct in efforts to control pink bollworm via the sterile insect technique and will boost genetic manipulation of this insect ...
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INHIBITION OF POLYMERASE CHAIN REACTION BY LITHIUM CHLORIDE
... The polymerase chain reaction (PCR) is a relatively simple technique that amplifies a DNA template to produce specific DNA fragments in in ...a reaction to perform [ 1 ]. A typical ...
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Use of the Reverse Transcription-Polymerase Chain Reaction for differential detection of two lineages of the canine distemper virus in Chile
... DNA ampli ication. The PCR technique contemplates a stage of DNA denaturation, followed by a stage of alignment of the starters and a inal stage of elongation to inish a cycle. The DNA denaturation phase was ...
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Association between polymorphisms in selected inflammatory response genes and the risk of prostate cancer
... using polymerase chain reaction-restriction fragment length polymorphism technique on 439 prostate cancer patients and 524 controls, and the association of each polymorphic genotype with ...
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Polymerase chain reaction for detection of Mycobacterium tuberculosis
... The aims of this study were to develop a simple, safe method for extraction of mycobacterial DNAs present in clinical samples and to develop a specific, sensitive PCR by using primers ba[r] ...
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Detection of leptospires in urine by polymerase chain reaction
... Clone pLBEc23s showed a strong hybridization with its homologous serovar hardjo, type hardjobovis, and with serovars balcanica, sejroe, polonica, dikkeni, nyanza, and saxkoebing.. Weaker[r] ...
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Polymerase chain reaction assay for avian polyomavirus
... Lanes 2, 3, 5, 7, and 9 contain the amplified products from purified DNA samples from CEFs infected with BFDV, pooled organ tissue from a nestling budgerigar, purified viral DNA from a l[r] ...
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Identification of meningococcal serosubtypes by polymerase chain reaction
... Southern hybridization experiments demonstrated that it was possible to identify genes encoding different serological variants of the meningococcal class 1 outer membrane protein by prob[r] ...
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Detection of Babesia microti by polymerase chain reaction
... In order to determine the sensitivity of PCR detection, we performed 1:10 serial dilutions on one of these specimens using uninfected human blood as the diluent.. The number of organisms[r] ...
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Detection of Chlamydia pneumoniae by polymerase chain reaction
... pneumoniae isolates tested were amplified by the HL-1-HR-1 primer pair or the HM-1-HR-1 primer pair, producing the expected 437- and 229-bp amplification products, respectively.. Reactio[r] ...
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