Preantral follicles
In vitro culture of bovine preantral follicles: a review
... bovine preantral follicles in vitro (estradiol: [135]; testosterone: ...isolated preantral follicles without affecting the prolif- eration of granulosa cells ...primordial follicles ...
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Onset and Heterogeneity of Responsiveness to FSH in Mouse Preantral Follicles in Culture
... antral follicles is well recognized, but its function in preantral growth is less ...mouse preantral follicles of increasing size, focusing particularly on growth rate and gene ...expression. ...
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The Effects of Different Concentrations of Leukemia Inhibitory Factor on the Development of Isolated Preantral Follicles from Fresh and Vitrified Mouse Ovaries
... The preantral follicles from fresh and vitrified-warmed ovaries with a diameter of 140-170 µm were mechanically isolated using 29-guage needle under ...Isolated follicles containing several layers of ...
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A protocol to isolate and qualify purified human preantral follicles in cases of acute leukemia, for future clinical applications
... 100 preantral follicles isolated with GMP grade collagenase NB6 were carefully ...isolated follicles would be embedded in fibrin ...isolated follicles was then mixed with 15 μl of thrombin ...
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Ultrastructure of ovarian preantral follicles and corpus luteum in Indian flying fox Pteropus giganteus (Brünnich)
... of preantral follicle had been observed in Pteropus ...large preantral follicles (type-4) was characterized by the presence of cortical granules in the cortical ...
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SET protein up-regulated testosterone production in the cultured preantral follicles
... The in vitro culture model of mouse preantral follicles mimicked the native ovary by maintaining similar struc- tural integrity. Hormones and paracrine factors produced by granulosal and/or theca cells can ...
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Granulosa cells regulate oocyte intracellular pH against acidosis in preantral follicles by multiple mechanisms
... result raises questions about the specificity of NEM in these experiments. We therefore also employed bafilomycin and concanamycin, two related macrolide antibiotics that are highly specific for V-ATPases (Bowman et al., ...
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Activin promotes oocyte development in ovine preantral follicles in vitro
... by preantral follicles in the present ...ovine follicles after antrum forma- tion. As sheep follicles have a much longer growth period compared with rodent follicles, there are likely ...
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Pioglitazone suppresses excessive follicular development in murine preantral follicles
... antral follicles pause in their ...murine preantral follicles. Secondary follicles (100-160 μ m in diameter) isolated from 6-week-old ICR mice were individually cultured for 13 ...within ...
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Exposure to cell phone induce oxidative stress in mice preantral follicles during in vitro cultivation: An experimental study
... In the cell phone exposed PFs, the TAC level was significantly lower (p ≤ 0.001) and MDA levels was significantly higher (p ≤ 0.001), compared tothe those of control group.. Conclusion: [r] ...
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Oocyte-granulosa-theca cell interactions during preantral follicular development
... of follicles to develop past the primary stage [43], demonstrating the importance of this growth factor in early follicular ...vulatory follicles [9], suggesting that GDF-9 is an important granulosa cell ...
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Towards a 3D culture of mouse ovarian follicles
... which preantral follicles (< two layers follicle cells) from 12-day-old mice were cultured on Co- star Transwell-COL membrane inserts treated with an equimolar mixture of type I and III collagen from ...
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Androgen potentiates the expression of FSH receptor and supports preantral follicle development in mice
... mouse preantral follicles via increased FSH ...in follicles is an essential factor for an- drogen to potentiate follicle ...mouse follicles seem to acquire FSH-dependency once they reach a ...
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Histomorphological Study of the Ovaries and Follicles Growth in Adult Female Albino Rats
... primary follicles, early primary follicle secondary and preantral follicles and less amount for large follicle which is represent antral follicles filled with the fluid and single cavity were ...
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Expression of the beta-2 adrenergic receptor (ADRB-2) in human and monkey ovarian follicles: a marker of growing follicles?
... monkey follicles (4 animals) were exposed to NE or the ADRB-2 agonist isoproterenol (ISO), and follicular development (size) was ...monkey follicles revealed ADRB-2 in GCs of primordial, primary, secondary ...
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The Invasion and Reproductive Toxicity of QDs-Transferrin Bioconjugates on Preantral Follicle in vitro
... the preantral follicles were cultured individually in 10 µL droplets of culture medium overlaid with mineral oil in 35 mm dishes (10 ...of follicles was observed and measured daily under inverted ...
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Calcium alginate microencapsulation of ovarian follicles impacts FSH delivery and follicle morphology
... Encapsulated preantral follicles cul- tured with FSH, maintain a well developed theca, a base- ment membrane, a normally configured granulosa compartment and a healthy appearing egg with a zona pellucida ...
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Expression and molecular consequences of inhibition of estrogen receptors in granulosa cells of bovine follicles
... To further investigate the roles of ESRs during follicu- lar growth, we performed in vivo intrafollicular adminis- tration of the ESRs antagonist fulvestrant in cows. Fulvestrant is known to disrupt the dimerization and ...
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Age-related morphometrical peculiarities of Lithuanian women’s primordial ovarian follicles
... ovarian follicles are formed, they enter the prim- ordial (“resting”) stage, which persists for a period of time that varies from follicle to ...primary follicles or become atretic ...primordial ...
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Quantitative bioluminescence imaging of transgene expression in intact porcine antral follicles in vitro
... The present study has not addressed effects of gene delivery methods, bioluminescence imaging, and long term in vitro culture conditions on ovarian follicles. We are cur- rently pursuing studies to optimize ...
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