pregenomic RNA
A Secondary Structure That Contains the 5′ and 3′ Splice Sites Suppresses Splicing of Duck Hepatitis B Virus Pregenomic RNA
... called pregenomic RNA (pgRNA) (or genomic RNA, in the case of retroviruses), is the template for reverse tran- scription, as well as the mRNA for proteins required for viral ...unspliced RNA ...
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Effects of mutations within and adjacent to the terminal repeats of hepatitis B virus pregenomic RNA on viral DNA synthesis.
... the pregenomic RNA (pgRNA) terminal redundancy, that were specifically recognized by a 65-kDa host factor, ...affecting RNA packaging or minus-strand ...
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Pregenomic RNA Is Required for Efficient Incorporation of Pol Polyprotein into Foamy Virus Capsids
... of RNA for Pol function (9, ...package pregenomic RNA were found to be deficient in cleavage despite coexpression of a functional protease (9, ...an RNA-mediated mechanism for the incorpora- ...
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Core Protein Phosphorylation Modulates Pregenomic RNA Encapsidation to Different Extents in Human and Duck Hepatitis B Viruses
... assay. RNA encapsidation within core protein particles was examined as described previously by Yu and Summers (21) with minor ...min. Pregenomic RNA was detected by hybrid- ization with a 32 ...
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A host factor that binds near the termini of hepatitis B virus pregenomic RNA.
... (HBV) pregenomic RNA (pgRNA) harbors sites governing many essential functions in the viral life cycle, including polyadenylation, translation, RNA encapsidation, and DNA ...redundant RNA. By ...
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Inhibition of Hepatitis B Virus Replication by MyD88 Involves Accelerated Degradation of Pregenomic RNA and Nuclear Retention of Pre-S/S RNAs
... ε RNA signal, which eliminates pregenomic RNA packaging and DNA rep- lication ...the pregenomic RNA is driven by the cytomegalovirus (CMV) promoter and the Tet promoter, ...HBV ...
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RNA-Binding Motif Protein 24 (RBM24) Is Involved in Pregenomic RNA Packaging by Mediating Interaction between Hepatitis B Virus Polymerase and the Epsilon Element
... 3.5-kb pregenomic RNA (pgRNA)/preC RNA and ...(DHBV) RNA mutations have no effect on Pol binding, although they abolish its function in pgRNA packaging and protein priming (10, ...
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Evidence that the 5′-End Cap Structure Is Essential for Encapsidation of Hepatitis B Virus Pregenomic RNA
... cap-free RNA in ...HBV pregenomic RNA can be transcribed under the control of the T7 promoter ...that RNA could be made at low levels from the T7 promoter by host RNA polymerase II ...
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High serum levels of pregenomic RNA reflect frequently failing reverse transcription in hepatitis B virus particles
... HBV RNA in serum was likely of full length, we used digital PCR to analyse the quantitative relationship between the two full-length RNA species, precore RNA and ...precore RNA and one ...
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Hepatitis B Virus Core Protein Dephosphorylation Occurs during Pregenomic RNA Encapsidation
... ABSTRACT Hepatitis B virus (HBV) core protein consists of an N-terminal assembly domain and a C-terminal domain (CTD) with seven conserved serines or threonines that are dynamically phosphorylated/dephosphorylated during ...
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Rice tungro bacilliform virus open reading frames II and III are translated from polycistronic pregenomic RNA by leaky scanning.
... higher RNA levels than the other ...RTBV pregenomic RNA in which the positions of the splice donor (SD) and splice acceptor (SA) sites for generation of the ORF IV mRNA and start codons of the ORFs ...
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The Topology of Hepatitis B Virus Pregenomic RNA Promotes Its Replication
... Previous analysis of hepatitis B virus (HBV) indicated base pairing between two cis-acting sequences, the 5 ⴕ half of the upper stem of and , contributes to the synthesis of minus-strand DNA. Our goal was to identify ...
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Multiple widely spaced elements determine the efficiency with which a distal cistron is expressed from the polycistronic pregenomic RNA of figwort mosaic caulimovirus.
... polycistronic RNA to be translated in the presence of P6, the caulimovirus pregenomic RNA leader re- gion is not essential for polycistronic gene expression but rather fulfills a regulatory ...
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The Interface between Hepatitis B Virus Capsid Proteins Affects Self-Assembly, Pregenomic RNA Packaging, and Reverse Transcription
... Hepatitis B virus (HBV) capsid proteins (Cps) assemble around the pregenomic RNA (pgRNA) and viral reverse transcriptase (P). pgRNA is then reverse transcribed to double-stranded DNA (dsDNA) within the ...
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cis-acting sequences required for encapsidation of duck hepatitis B virus pregenomic RNA.
... b Cells were transfected with WT DHBV DNA, D3-0X118 DNA, or both, and the genomic RNA present in equal portions of preparations of total cellular polyA+ RNA and encapsidated RNA were qua[r] ...
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Pregenomic RNA encapsidation analysis of eleven missense and nonsense polymerase mutants of human hepatitis B virus.
... 2, upon transfection of the tandem dimer of the HBV mutant genomes into Huh7 hepatoma cells, we analyzed the total RNA and the core particle-associated RNA prepared from the transfected [r] ...
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Identification of a strong enhancer element upstream from the pregenomic RNA start site of the duck hepatitis B virus genome.
... The HepG2 cells transfected with the resulting plasmid DH10 contain- gene, ing 192 bp of showed an DHBV genome increase nucleotides 2159 of CAT to 2351 also activity comparable to that o[r] ...
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Interaction of the Cauliflower Mosaic Virus Coat Protein with the Pregenomic RNA Leader
... Hybrid proteins. Hybrid protein 1 (see Fig. 2A), containing the DNA binding domain (LexA) and the RNA binding domain of the MS2 coat protein, is integrated into the genome of the yeast strain L40-coat. To obtain ...
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Hepatocyte Nuclear Factor 3β Inhibits Hepatitis B Virus Replication In Vivo
... host RNA polymerase (5, ...from pregenomic RNA converts the immature nucleocapsids into mature nucleocapsids that bind the envelope antigen polypep- tides in the endoplasmic reticulum membrane and ...
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Differential regulation of the pre-C and pregenomic promoters of human hepatitis B virus by members of the nuclear receptor superfamily.
... and pregenomic RNAs of human hepatitis B virus (HBV) is directed by two overlapping yet separate promoters ...and pregenomic RNAs was affected both in transfected hepatoma cells and in a cell-free ...
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