a Preparation of epithelial cells from the rectal
Lactoferrin translocates to the nucleus of bovine rectal epithelial cells in the presence of Escherichia coli O157:H7
... derived from milk translocated to the nucleus of rectal epithelial cells as confirmed by 3D confocal fluorescence analysis and confocal Raman ...the rectal epithelial ...
11
The effects of extracellular sodium chloride on the activity and expression of Na,K ATPase in primary cultures of dogfish (Scyliorhinus canicula) rectal gland epithelial cells
... chloride from the plasma into the external ...dogfish rectal gland. 1.1 SALT SECRETING EPITHELIA Epithelia are sheets of cells that line body cavities such as the lumen of the intestine, the kidney ...
341
A new method for culturing dogfish shark (Scyliorhinus canicula) rectal gland epithelial cells
... improvement as cells typically covered a full well. Complete monolayers were achieved typically within 9-10 days with attachment and growth beginning at approximately two days. This technique could still be ...
205
IN VITRO STUDY OF HUMAN RECTAL EPITHELIAL CELLS I ATYPICAL ZONE OF H3 THYMIDINE INCORPORATION IN MUCOSA OF MULTIPLE POLYPOSIS
... though it was not possible to follow this tissue for several days, one might roughly estimate that the mean generation time of these cells should be at least 3 to 4 times this interval o[r] ...
8
PRIMARY CULTURES OF EPITHELIAL CELLS FROM RAINBOW TROUT GILLS
... However, epithelial cells in suspension lose their polarity and, since the different properties of the apical and the basolateral membranes is a key feature of epithelial cells, this severely ...
14
Development of a lung slice preparation for recording ion channel activity in alveolar epithelial type I cells
... alveolar epithelial cells have been almost exclusively limited either to direct observa- tion from freshly purified and cultured cell populations or by inference from in vivo/ex vivo ...
10
Neutrophils from severe asthmatic patients induce epithelial to mesenchymal transition in healthy bronchial epithelial cells
... Immunofluorescence Some of the isolated neutrophils were used to prepare slides for immunostaining by cytospin. Neutrophils were suspended in RPMI medium at 500,000 cells per milli- litre and 80 μL was used per ...
14
Brushed nasal epithelial cells are a surrogate for bronchial epithelial CFTR studies
... derived from CF patients to test CFTR modulator responsiveness include explant HBEs derived at the time of transplant and intestinal organoids isolated from rectal tissue (11, 22– ...require ...
15
Immortalized epithelial cells from human autosomal dominant polycystic kidney cysts
... RNA isolation and RT-PCR. The cells were grown to con- fluence in 100-mm culture plates or 75-cm 2 flasks, then scraped, washed in PBS, and centrifuged. The cell pellet was snap-frozen in dry-ice ethanol, and ...
16
Microvesicles preparation from mesenchymal stem cells
... stem cells reached to 80% confluence, the DMEM medium was replaced with fresh RPMI de- prived of FBS and added ...dead cells and large cell debris by consecutive centrifugations at increas- ing ...
8
Nipah Virus Entry and Egress from Polarized Epithelial Cells
... polarized epithelial cells is an intrinsic property, we analyzed the M protein distribu- tion in polarized cells in the absence of NiV ...M-expressing cells were seeded onto FIG 4 Kinetics of ...
12
Extracellular vesicles from mast cells induce mesenchymal transition in airway epithelial cells
... However, the role of EV surface-associated DNA in the regulation of EMT as suggested in this study remains to be confirmed in further studies. To evaluate whether the morphological changes in- duced in epithelial ...
13
The in vitro differentiation of prostate epithelial cells and gonadal adipocytes from mouse embryonic stem cells
... Chapter 6: Nile Blue is a cell permeable sensor of intracellular free fatty acids in live cells. 6.1 Introduction In Chapter 5, a flow cytometric method was presented that can analyse adipocytes at the single cell ...
403
Epithelial Sodium Channels in Pulmonary Epithelial Progenitor and Stem Cells
... result from or are associated with the dysfunction or dysregulation of ENaC, and the regeneration of epithelial cells and the restoration of ion transport are two key steps in recovery from ...
5
Vectorial release of poliovirus from polarized human intestinal epithelial cells.
... The latter observation suggests that although monensin- and ammonium chloride-sensitive processes may be involved in the release of a proportion of progeny virus (particularly at the bas[r] ...
9
DNA purification from buccal epithelial cells by ammonium acetate method
... Buccal cells are an excellent source of DNA for diagnosis and large-scale molecular epidemiological ...DNA from buccal cells, but cell collection by mouthwash seems to give higher yields than many ...
40
Directional entry and release of Zika virus from polarized epithelial cells
... Caco-2 cells serve as an excellent model to study the permeability barrier since they readily form tight junc- tions when grown on a semipermeable barrier [25, ...Caco-2 cells have been used to investigate ...
8
Isolation of high density lipoproteins from rat intestinal epithelial cells
... homogenates were subjected to nitrogen cavitation and a cytoplasmic fraction was prepared. Lipoproteins were isolated from both preparations by sequential ultracentrifugation. When the HDL fraction (1.07-1.21 ...
11
Proteomic Analysis of Nasal Epithelial Cells from Cystic Fibrosis Patients
... inferior turbinate were obtained from individuals who underwent turbinectomy for snoring. All the patients did not mention any allergic background and were negatively tested with skin prick tests. All non-CF ...
14
IGFBP-5 enhances epithelial cell adhesion and protects epithelial cells from TGFβ1-induced mesenchymal invasion
... in epithelial cells whereas, in mesenchymal cells, IGFBP-5 decreased adhesion and ...simulating epithelial-mesenchymal boundaries, IGFBP-5 was able to antagonise the disruptive transgressions ...
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