Primary cultures
A collagenolytic system produced by primary cultures of rheumatoid nodule tissue
... A collagenase and a neutral protease have been insolated and characterized from primary cultures obtained from rheumatoid subcutaneous nodules. Release of both active enzymes was maximal between the 3rd and ...
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Infection of primary cultures of human neural cells by human coronaviruses 229E and OC43.
... infected primary cultures, presumably astro- cytes ...cell cultures consisted of 80 to 90% oligodendrocytes and (ii) the immunofluorescence results showing that the only cells exhib- iting ...
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Progesterone reduces cell survival in primary cultures of endometrioid ovarian cancer
... study, primary cultures of ovarian carcinoma obtained from 35 patients diagnosed with epithelial ovarian cancer were evaluated for cell survival after treatment with 10 − 8 M of 17 β -estradiol, ...
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Theiler's Virus Infection of Primary Cultures of Bone Marrow-Derived Monocytes/Macrophages
... Although they offer obvious practical advantages, tissue cul- ture-adapted macrophage cell lines are significantly different from the original cells. Furthermore, as already mentioned, susceptibility to persistence of ...
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PRIMARY CULTURES OF EPITHELIAL CELLS FROM RAINBOW TROUT GILLS
... The growth curve was established from the protein content of the culture dishes. Although we found a strong correlation between the amount of protein and the number of cells (Fig. 1), the critical assumption is that the ...
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Action of the Disintegrin Contortrostatin on Breast Cancer Cell Primary Cultures
... Drug assays, in general, may be qualitative or quanti- tative. In the context of clinical envenoming, a quantita- tive assay is only useful if the degree of pathological damage in vivo can be correlated with an amount of ...
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Replication of human hepatitis delta virus in primary cultures of woodchuck hepatocytes.
... 2891 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We obtained two lines of evidence that monolayer cultures of primary woodchuck hepatocytes support replication of t[r] ...
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Regulation of the metabolism of 25 hydroxyvitamin D3 in primary cultures of chick kidney cells
... 24,25(OH)2D3 produced similar effects, but only in the absence of fetal calf serum. R and S isomers of 1,24,25(OH)3D3 were about fives times less active than 1,25(OH)2D3. Bovine parathyroid hormone stimulated the 1- and ...
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Regulation of lipoprotein lipase in primary cultures of isolated human adipocytes
... To study the regulation of adipose tissue lipoprotein lipase LPL in human adipocytes, omental adipose tissue was obtained from healthy subjects and digested in collagenase.. The isolated[r] ...
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N–nitrosodiethylamine cytochrome P450 induction and cytotoxicity evaluation in primary cultures of rat hepatocytes
... The primary routes of potential human exposure to N-nitrosodiethylamine (NDEA) are ingestion, inhala- tion, and dermal ...rat primary hepatocytes cultures, were used to understand the CYP induction ...
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Prevention of the β-amyloid peptide-induced inflammatory process by inhibition of double-stranded RNA-dependent protein kinase in primary murine mixed co-cultures
... Co-cultures were treated with either C16 (specific inhibi- tor of PKR) at different concentrations (210 nM (IC50) and 1 μ M) or DMSO (vehicle of C16) at less than 1%, in serum-free MEM:Neurobasal (1:1)/1% ...
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The characterization of the flavin-containing monooxygenase gene family of man
... I would like to thank Dr. Colin Dolphin for his help, advice and comments especially with the RNase protection and enzyme activity assays; Dr. Mike O'Hare and the members of his lab at the Department of Surgery, ...
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Establishing and maintaining primary cell cultures derived from the ctenophore Mnemiopsis leidyi
... of primary cell cultures isolated from adult Mnemiopsis leidyi, a lobate ...Our primary cell cultures are derived from tissue explants or enzymatically dissociated cells, and maintained in a ...
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Management and potentialities of primary cancer cultures in preclinical and translational studies
... of primary liposarcoma and compared it with patient histology ...of primary cultures, increasing the amount of tumor material obtained by sample digestion ...scaffold cultures a useful ...
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sustains human glioma cell growth and survival through EGF signaling
... 3D primary cultures derived from representative GBM ...promote primary cultures survival and growth in vitro and the effect is likely to occur though the Prostaglandin E2 receptor ...
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Betanodavirus infection in primary neuron cultures from sole
... the primary culture of neurons from Senegalese sole, which made it possible to further study virus‑host cell ...neuron primary cultures and E‑11 ...sole primary neuronal culture protocol ...
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Stromal upregulation of lateral epithelial adhesions: Gene expression analysis of signalling pathways in prostate epithelium
... This research highlights the difficulties faced by a cell biologist trying to select the most appropriate model system for their research. In our work we prefer to vali- date all our experiments using primary ...
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Cell Survival Assays for Individualised Chemotherapy in Primary Glioma Cultures—Colourmetric or Luminescent?
... and primary cultures from ovarian and gastric cancers have demonstrated that this assay is able to exhibit high sensitivity even at very low cell ...
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Characterization of single non inactivating potassium channels in primary neuronal cultures of Drosophila
... Permeability and gating properties of single, non-inactivating K + channel currents in neurons of eag, a mutant which has defects in the delayed rectifier K + channel, were indistinguish[r] ...
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Primary isolation and serial passage of hepatitis A virus strains in primate cell cultures
... Tube cultures of BS-C-1 cells were infected with 10fold dilutions of a strain PA-21 virus seed passage six, held at 32, 35, or 39°C, and tested at 7-day intervals by in situ radioimmunoa[r] ...
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