Primers used for RT-PCR
HiPer RT-PCR Teaching Kit
... of RT-PCR, the cDNA is amplified through a standard PCR by using a gene specific primer and a thermostable DNA ...specific primers can ...specific primers are annealed that are ...
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Development of a Microplate Lectin Capture RT PCR (MLC RT PCR) for the Detection of Avian Infectious Bronchitis Virus
... of primers used in the ...MLC- RT-PCR, although the interactions of Con A with mo- lecular components of these viruses can not be ruled ...
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Real-time quantitative RT -PCR (Taqman)
... d) Primer optimisation Reactions to amplify two different segments in the same tube share common reagents. If the two segments have different initial copy numbers, it is possible for the more abundant species to use up ...
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PrimerSeq: Design and visualization of RT-PCR primers for alternative splicing using RNA-seq data.
... is used by both isoforms before the skipped exon of ...design primers for all types of AS events, such as exon skipping, alternative splice sites and mutually exclusive ...
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PrimerSeq: Design and Visualization of RT-PCR Primers for Alternative Splicing Using RNA-seq Data
... is used by both isoforms before the skipped exon of ...design primers for all types of AS events, such as exon skipping, alternative splice sites and mutually exclusive ...
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An Endogenous Murine Leukemia Viral Genome Contaminant in a Commercial RT PCR Kit is Amplified Using Standard Primers for XMRV
... one-step RT-PCR ...one-step RT-PCR kits from Invitrogen, TaKaRa, Promega and QIAGEN in ...widely used in XMRV studies were ...one-step RT-PCR kit from Invitrogen were ...
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Development of a Universal Intimin Antiserum and PCR Primers
... we used a conserved region of intimin (Int 388–667 ) located upstream of the cell binding ...the PCR primers synthesized to generate Int 388–667 gave a specific PCR product with as the ...
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Impact of Enzymes and Primers on the PCR Amplification of Some Goatfishes
... as PCR inhibitors. Here, two instruments were used to measure concentrations and purities of DNA extracted from three species of the family Mullidae (Mullus barbatus, Linnaeus, 1758; Mullus surmuletus, ...
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A database of PCR primers for the chloroplast genomes of higher plants
... Positioning primers in sequenced chloroplast genomes BLASTALL (obtained from NCBI, USA [19]), was used to search for homologies of the primers in 13 chloroplasts (from GenBank, December 2005, except ...
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Universal Primers Used for Detection of Bacterial Meningitis
... Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran ARTICLE INFO ABSTRACT Article type: Original Article Background: Acute bacterial meningitis is among serious ...
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Superiority of Digital Reverse Transcription PCR (RT PCR) over Real Time RT PCR for Quantitation of Highly Divergent Human Rhinoviruses
... qualitative RT-PCR, an accurate quantification method is needed to determine if HRV viral load is associated with viral transmission and pathogenicity and with patient symptoms and outcomes, as has been ...
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PCR Based Genotyping of Mycobacterium tuberculosis with New GC Rich Repeated Sequences and IS6110 Inverted Repeats Used as Primers
... a PCR-based epidemiological tool for the differentiation of Mycobacterium tuberculosis ...designed primers Mtb1 (5 ⴕ -CCG-GCG-GGG-CCG-GCG-G) and Mtb2 (5 ⴕ -CGG-CGG-CAA-CGG-CGG-C) targeting frequently ...
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COVID-19 Mutation RT-PCR Assays
... Mutation RT-PCR Assays targets one viral sequence of the SARS-CoV-2 (Table ...reverse primers surrounding the site of potential variation and an EasyBeacon labelled either with FAM™, HEX™, Cy5™ or ...
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PRISE2: software for designing sequence-selective PCR primers and probes.
... sequence-selective PCR primers and ...the primers are supposed to amplify, as well as non-target sequences that should not be ...of primers, including length, GC content, and ...candidate ...
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Detection of Mycobacterium ulcerans by real-time PCR with improved primers
... results, PCR sensitivity was more signifi- cantly improved by new primers for three Japanese iso- ...conventional primers than other ...new primers improved the sensitivity of M. ulcerans ...
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A systematic guideline for developing the best real-time PCR primers
... the primers must detect every alternative transcript and splicing variant of the queried ...of PCR cycling ...organism used to over- express and purify the ...the primers controls the start ...
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Incorrect identification of recent Asian strains of Coxsackievirus A16 as human enterovirus 71: Improved primers for the specific detection of human enterovirus 71 by RT PCR
... of primers for this purpose were described by Brown and colleagues [8] and one of these pairs (159S/162A) has been extensively used to investigate recent HEV71 ...important primers can occasionally ...
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Cost Effective Real Time Reverse Transcriptase PCR (RT PCR) To Screen for Dengue Virus followed by Rapid Single Tube Multiplex RT PCR for Serotyping of the Virus
... effectively used during the dengue outbreak in Singapore in 2005. A cost-effective RT-PCR assay based on SYBR green I has been developed for general screening of samples from suspected ...of ...
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Design of an improved set of oligonucleotide primers for genotyping MeCP2tm1.1BirdKO mice by PCR
... a PCR product with either one pair of primer or the other (P5-P6 for the KO, P5-P7 for the ...we used was that genotyping of males frequently gave rise to bands in both sets of PCR reactions, with ...
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Specific Primers for Rapid Detection of Microsporum audouinii by PCR in Clinical Samples
... “MA1” primers. The utility of the MA1_F and MA1_R primers as a tool for iden- tifying ...ellite primers M13, (GTG) 5 , and (GACA) 4 . All strains used in this study were tested with these ...
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